Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF903 and GFF904 are separated by 13 nucleotides GFF904 and GFF905 are separated by 8 nucleotides GFF905 and GFF906 are separated by 11 nucleotides GFF906 and GFF907 are separated by 109 nucleotides
GFF903 - Acetolactate synthase large subunit (EC 2.2.1.6), at 213,935 to 215,713
GFF903
GFF904 - hypothetical protein, at 215,727 to 216,158
GFF904
GFF905 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 216,167 to 216,943
GFF905
GFF906 - Transcriptional regulator, at 216,955 to 217,422
GFF906
GFF907 - Transcriptional regulator, LysR family, at 217,532 to 218,425
GFF907
Position (kb) 216
217 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 215.202 kb on - strand, within GFF903 at 215.261 kb on + strand, within GFF903 at 215.262 kb on - strand, within GFF903 at 215.417 kb on + strand, within GFF903 at 215.417 kb on + strand, within GFF903 at 215.417 kb on + strand, within GFF903 at 215.418 kb on - strand, within GFF903 at 215.473 kb on + strand, within GFF903 at 215.473 kb on + strand, within GFF903 at 215.474 kb on - strand, within GFF903 at 215.474 kb on - strand, within GFF903 at 215.699 kb on + strand at 215.700 kb on - strand at 215.700 kb on - strand at 215.923 kb on + strand, within GFF904 at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.430 kb on - strand, within GFF905 at 216.551 kb on + strand, within GFF905 at 216.551 kb on + strand, within GFF905 at 216.552 kb on - strand, within GFF905 at 216.568 kb on - strand, within GFF905 at 216.587 kb on + strand, within GFF905 at 216.587 kb on + strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.618 kb on + strand, within GFF905 at 216.690 kb on + strand, within GFF905 at 216.690 kb on + strand, within GFF905 at 216.782 kb on + strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.811 kb on - strand, within GFF905 at 216.811 kb on - strand, within GFF905 at 216.965 kb on + strand at 216.966 kb on - strand at 217.229 kb on + strand, within GFF906 at 217.229 kb on + strand, within GFF906 at 217.229 kb on + strand, within GFF906 at 217.230 kb on - strand, within GFF906 at 217.230 kb on - strand, within GFF906 at 217.230 kb on - strand, within GFF906 at 217.434 kb on + strand at 217.434 kb on + strand at 217.434 kb on + strand at 217.434 kb on + strand at 217.435 kb on - strand at 217.435 kb on - strand at 217.436 kb on + strand at 217.437 kb on - strand at 217.437 kb on - strand at 217.437 kb on - strand at 217.458 kb on - strand at 217.599 kb on + strand at 217.599 kb on + strand at 217.599 kb on + strand at 217.815 kb on + strand, within GFF907 at 217.815 kb on + strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 215,202 - GFF903 0.71 -1.1 215,261 + GFF903 0.75 -0.3 215,262 - GFF903 0.75 +1.9 215,417 + GFF903 0.83 +0.2 215,417 + GFF903 0.83 -0.5 215,417 + GFF903 0.83 -0.4 215,418 - GFF903 0.83 -2.2 215,473 + GFF903 0.86 -0.7 215,473 + GFF903 0.86 -0.7 215,474 - GFF903 0.87 -0.7 215,474 - GFF903 0.87 +1.4 215,699 + -2.6 215,700 - +0.6 215,700 - -0.9 215,923 + GFF904 0.45 -0.2 216,237 + +0.3 216,237 + -0.7 216,237 + +0.1 216,237 + +1.1 216,237 + +0.2 216,237 + +0.6 216,237 + -0.4 216,237 + -0.0 216,238 - -0.6 216,238 - +0.6 216,238 - -0.4 216,238 - +0.3 216,238 - -2.2 216,430 - GFF905 0.34 +0.3 216,551 + GFF905 0.49 -0.7 216,551 + GFF905 0.49 +0.6 216,552 - GFF905 0.50 -0.8 216,568 - GFF905 0.52 +1.6 216,587 + GFF905 0.54 +0.2 216,587 + GFF905 0.54 -1.2 216,588 - GFF905 0.54 -3.0 216,588 - GFF905 0.54 -1.8 216,588 - GFF905 0.54 -1.0 216,618 + GFF905 0.58 -0.0 216,690 + GFF905 0.67 -2.3 216,690 + GFF905 0.67 -0.4 216,782 + GFF905 0.79 -0.8 216,783 - GFF905 0.79 -0.8 216,783 - GFF905 0.79 +0.0 216,783 - GFF905 0.79 -0.5 216,783 - GFF905 0.79 +1.2 216,811 - GFF905 0.83 -0.1 216,811 - GFF905 0.83 -1.3 216,965 + -0.2 216,966 - -0.6 217,229 + GFF906 0.59 -1.4 217,229 + GFF906 0.59 +0.3 217,229 + GFF906 0.59 -0.4 217,230 - GFF906 0.59 -0.8 217,230 - GFF906 0.59 -0.8 217,230 - GFF906 0.59 -2.1 217,434 + -1.0 217,434 + +2.8 217,434 + +0.2 217,434 + +1.6 217,435 - -0.9 217,435 - -0.5 217,436 + -1.6 217,437 - +0.4 217,437 - -0.2 217,437 - -1.2 217,458 - -0.2 217,599 + +0.1 217,599 + -0.0 217,599 + +0.6 217,815 + GFF907 0.32 -0.5 217,815 + GFF907 0.32 -0.3 217,816 - GFF907 0.32 -0.1 217,816 - GFF907 0.32 -0.2 217,816 - GFF907 0.32 -0.6 217,816 - GFF907 0.32 +0.6 217,816 - GFF907 0.32 -2.1
Or see this region's nucleotide sequence