Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF838 and GFF839 are separated by 317 nucleotides GFF839 and GFF840 overlap by 4 nucleotides GFF840 and GFF841 are separated by 11 nucleotides
GFF838 - hypothetical protein, at 143,452 to 144,552
GFF838
GFF839 - Retron-type RNA-directed DNA polymerase (EC 2.7.7.49), at 144,870 to 146,816
GFF839
GFF840 - no description, at 146,813 to 147,082
GFF840
GFF841 - zinc finger, SWIM-type, at 147,094 to 148,800
GFF841
Position (kb) 144
145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 144.269 kb on + strand, within GFF838 at 144.531 kb on - strand at 144.599 kb on + strand at 144.763 kb on + strand at 144.763 kb on + strand at 144.763 kb on + strand at 144.764 kb on - strand at 144.783 kb on + strand at 144.784 kb on - strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.037 kb on - strand at 145.037 kb on - strand at 145.037 kb on - strand at 145.037 kb on - strand at 145.427 kb on - strand, within GFF839 at 145.427 kb on - strand, within GFF839 at 145.778 kb on - strand, within GFF839 at 145.903 kb on + strand, within GFF839 at 145.904 kb on - strand, within GFF839 at 146.176 kb on + strand, within GFF839 at 146.435 kb on - strand, within GFF839 at 146.435 kb on - strand, within GFF839 at 146.919 kb on - strand, within GFF840 at 146.981 kb on + strand, within GFF840 at 147.092 kb on + strand at 147.092 kb on + strand at 147.092 kb on + strand at 147.503 kb on + strand, within GFF841 at 147.504 kb on - strand, within GFF841 at 147.504 kb on - strand, within GFF841 at 147.527 kb on + strand, within GFF841
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 144,269 + GFF838 0.74 -1.1 144,531 - +0.4 144,599 + +0.2 144,763 + -0.7 144,763 + -0.2 144,763 + +0.6 144,764 - +1.2 144,783 + -2.0 144,784 - -1.2 145,036 + -0.0 145,036 + +0.6 145,036 + -1.4 145,036 + -0.1 145,036 + +0.2 145,037 - +2.9 145,037 - -0.2 145,037 - +0.1 145,037 - -0.8 145,427 - GFF839 0.29 -1.0 145,427 - GFF839 0.29 +1.2 145,778 - GFF839 0.47 -0.0 145,903 + GFF839 0.53 -0.0 145,904 - GFF839 0.53 -1.0 146,176 + GFF839 0.67 -0.6 146,435 - GFF839 0.80 -0.7 146,435 - GFF839 0.80 -2.5 146,919 - GFF840 0.39 -2.3 146,981 + GFF840 0.62 -0.2 147,092 + +0.2 147,092 + -0.1 147,092 + +0.4 147,503 + GFF841 0.24 +0.4 147,504 - GFF841 0.24 -0.2 147,504 - GFF841 0.24 -0.3 147,527 + GFF841 0.25 -0.4
Or see this region's nucleotide sequence