Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF76 and GFF77 overlap by 7 nucleotides GFF77 and GFF78 are separated by 1 nucleotides GFF78 and GFF79 are separated by 72 nucleotides GFF79 and GFF80 are separated by 42 nucleotides
GFF76 - Alkanesulfonates transport system permease protein, at 93,161 to 94,228
GFF76
GFF77 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 94,222 to 95,025
GFF77
GFF78 - Cytochrome c family protein, at 95,027 to 96,190
GFF78
GFF79 - Dodecin, a flavin storage/sequestration protein, at 96,263 to 96,469
GFF79
GFF80 - no description, at 96,512 to 98,155
GFF80
Position (kb) 95
96
97 Strain fitness (log2 ratio) -2
-1
0
1 at 94.080 kb on - strand, within GFF76 at 94.080 kb on - strand, within GFF76 at 94.109 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.122 kb on - strand at 94.122 kb on - strand at 94.122 kb on - strand at 94.122 kb on - strand at 94.147 kb on - strand at 94.534 kb on + strand, within GFF77 at 94.534 kb on + strand, within GFF77 at 94.535 kb on - strand, within GFF77 at 94.535 kb on - strand, within GFF77 at 95.439 kb on - strand, within GFF78 at 96.206 kb on + strand at 96.206 kb on + strand at 96.278 kb on + strand at 96.278 kb on + strand at 96.279 kb on - strand at 96.279 kb on - strand at 96.279 kb on - strand at 96.458 kb on - strand at 96.540 kb on + strand at 96.570 kb on + strand at 96.570 kb on + strand at 96.571 kb on - strand at 96.624 kb on - strand at 96.672 kb on + strand at 96.673 kb on - strand at 96.701 kb on + strand, within GFF80 at 96.702 kb on - strand, within GFF80 at 96.846 kb on + strand, within GFF80 at 96.847 kb on - strand, within GFF80 at 96.847 kb on - strand, within GFF80 at 96.847 kb on - strand, within GFF80 at 96.909 kb on + strand, within GFF80 at 96.910 kb on - strand, within GFF80 at 96.910 kb on - strand, within GFF80 at 97.039 kb on - strand, within GFF80
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 94,080 - GFF76 0.86 -0.3 94,080 - GFF76 0.86 -0.2 94,109 + GFF76 0.89 -0.0 94,121 + GFF76 0.90 -0.4 94,121 + GFF76 0.90 +1.4 94,121 + GFF76 0.90 -0.1 94,121 + GFF76 0.90 -0.3 94,121 + GFF76 0.90 +0.6 94,122 - +0.1 94,122 - -1.0 94,122 - -0.3 94,122 - +0.7 94,147 - +1.2 94,534 + GFF77 0.39 -1.8 94,534 + GFF77 0.39 +0.0 94,535 - GFF77 0.39 -0.8 94,535 - GFF77 0.39 -0.1 95,439 - GFF78 0.35 -0.7 96,206 + +0.4 96,206 + -0.2 96,278 + -0.7 96,278 + -0.7 96,279 - -1.0 96,279 - -0.2 96,279 - -0.7 96,458 - +0.2 96,540 + +1.2 96,570 + -2.0 96,570 + +0.6 96,571 - -0.6 96,624 - -0.0 96,672 + -0.2 96,673 - -0.6 96,701 + GFF80 0.11 -1.0 96,702 - GFF80 0.12 +0.3 96,846 + GFF80 0.20 -0.3 96,847 - GFF80 0.20 -0.5 96,847 - GFF80 0.20 -0.3 96,847 - GFF80 0.20 -1.2 96,909 + GFF80 0.24 -0.2 96,910 - GFF80 0.24 +0.2 96,910 - GFF80 0.24 -0.4 97,039 - GFF80 0.32 -2.2
Or see this region's nucleotide sequence