Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7370 and GFF7371 are separated by 94 nucleotides GFF7371 and GFF7372 are separated by 1 nucleotides GFF7372 and GFF7373 are separated by 72 nucleotides
GFF7370 - PAS domain with LuxR-type DNA-binding domain, at 73 to 639
GFF7370
GFF7371 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 734 to 1,546
GFF7371
GFF7372 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 1,548 to 2,186
GFF7372
GFF7373 - CaiB/BaiF family protein, at 2,259 to 3,650
GFF7373
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1
2 at 0.122 kb on + strand at 0.122 kb on + strand at 0.123 kb on - strand at 0.123 kb on - strand at 0.123 kb on - strand at 0.417 kb on - strand, within GFF7370 at 0.417 kb on - strand, within GFF7370 at 0.485 kb on + strand, within GFF7370 at 0.485 kb on + strand, within GFF7370 at 0.660 kb on + strand at 0.661 kb on - strand at 0.663 kb on + strand at 0.664 kb on - strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.668 kb on - strand at 0.675 kb on + strand at 0.676 kb on - strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.747 kb on - strand at 0.965 kb on + strand, within GFF7371 at 0.965 kb on + strand, within GFF7371 at 0.965 kb on + strand, within GFF7371 at 0.966 kb on - strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.999 kb on - strand, within GFF7371 at 0.999 kb on - strand, within GFF7371 at 0.999 kb on - strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.361 kb on + strand, within GFF7371 at 1.361 kb on + strand, within GFF7371 at 1.361 kb on + strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.609 kb on - strand at 1.609 kb on - strand at 1.609 kb on - strand at 1.609 kb on - strand at 1.634 kb on + strand, within GFF7372 at 1.634 kb on + strand, within GFF7372 at 1.634 kb on + strand, within GFF7372 at 1.635 kb on - strand, within GFF7372 at 1.635 kb on - strand, within GFF7372 at 2.184 kb on + strand at 2.185 kb on - strand at 2.524 kb on + strand, within GFF7373 at 2.525 kb on - strand, within GFF7373
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 122 + -1.1 122 + -0.7 123 - -1.7 123 - -0.4 123 - -0.4 417 - GFF7370 0.61 +0.7 417 - GFF7370 0.61 -0.3 485 + GFF7370 0.73 +0.3 485 + GFF7370 0.73 +0.4 660 + +0.4 661 - -0.0 663 + -0.5 664 - +2.0 667 + +0.5 667 + -0.5 667 + -0.5 667 + -0.8 667 + +0.5 668 - +1.3 675 + +0.2 676 - -0.2 679 + -0.2 679 + -0.5 679 + -0.8 679 + -2.2 679 + -0.5 680 - -0.6 680 - -0.7 680 - +0.7 680 - -0.8 680 - +0.2 680 - +1.7 747 - +0.0 965 + GFF7371 0.28 -0.7 965 + GFF7371 0.28 -1.8 965 + GFF7371 0.28 -0.3 966 - GFF7371 0.29 +1.2 998 + GFF7371 0.32 -2.0 998 + GFF7371 0.32 -0.1 998 + GFF7371 0.32 -0.5 998 + GFF7371 0.32 +0.7 999 - GFF7371 0.33 -0.5 999 - GFF7371 0.33 -0.7 999 - GFF7371 0.33 -0.7 1,157 + GFF7371 0.52 +0.3 1,157 + GFF7371 0.52 +0.1 1,157 + GFF7371 0.52 +0.1 1,157 + GFF7371 0.52 -0.5 1,158 - GFF7371 0.52 -0.1 1,158 - GFF7371 0.52 +1.1 1,158 - GFF7371 0.52 -0.3 1,158 - GFF7371 0.52 -1.1 1,158 - GFF7371 0.52 +0.4 1,158 - GFF7371 0.52 -0.4 1,158 - GFF7371 0.52 +0.2 1,361 + GFF7371 0.77 -0.7 1,361 + GFF7371 0.77 -0.3 1,361 + GFF7371 0.77 -1.1 1,362 - GFF7371 0.77 -0.0 1,362 - GFF7371 0.77 +0.1 1,362 - GFF7371 0.77 -1.8 1,362 - GFF7371 0.77 -2.0 1,362 - GFF7371 0.77 +0.1 1,362 - GFF7371 0.77 +0.4 1,362 - GFF7371 0.77 -0.1 1,362 - GFF7371 0.77 -1.7 1,608 + -0.1 1,608 + +0.7 1,608 + +0.3 1,608 + -0.7 1,608 + -1.0 1,608 + -0.1 1,608 + +0.8 1,608 + -0.8 1,609 - -0.6 1,609 - +1.7 1,609 - -1.3 1,609 - +0.1 1,634 + GFF7372 0.13 +0.8 1,634 + GFF7372 0.13 +0.7 1,634 + GFF7372 0.13 -1.0 1,635 - GFF7372 0.14 +0.7 1,635 - GFF7372 0.14 -0.2 2,184 + -0.8 2,185 - -0.5 2,524 + GFF7373 0.19 -0.2 2,525 - GFF7373 0.19 -0.3
Or see this region's nucleotide sequence