Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7344 and GFF7345 are separated by 137 nucleotides GFF7345 and GFF7346 overlap by 4 nucleotides GFF7346 and GFF7347 are separated by 3 nucleotides
GFF7344 - Transcriptional regulator, LysR family, at 12,232 to 13,221
GFF7344
GFF7345 - Periplasmic aromatic aldehyde oxidoreductase, iron-sulfur subunit YagT, at 13,359 to 14,015
GFF7345
GFF7346 - Periplasmic aromatic aldehyde oxidoreductase, FAD binding subunit YagS, at 14,012 to 14,962
GFF7346
GFF7347 - Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR, at 14,966 to 17,161
GFF7347
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.556 kb on - strand, within GFF7344 at 12.605 kb on + strand, within GFF7344 at 12.605 kb on + strand, within GFF7344 at 12.606 kb on - strand, within GFF7344 at 12.721 kb on + strand, within GFF7344 at 12.722 kb on - strand, within GFF7344 at 13.354 kb on + strand at 13.354 kb on + strand at 13.354 kb on + strand at 13.355 kb on - strand at 13.876 kb on - strand, within GFF7345 at 13.953 kb on + strand at 13.953 kb on + strand at 14.373 kb on - strand, within GFF7346 at 14.693 kb on + strand, within GFF7346 at 14.703 kb on + strand, within GFF7346 at 14.703 kb on + strand, within GFF7346 at 14.704 kb on - strand, within GFF7346 at 14.704 kb on - strand, within GFF7346
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 12,556 - GFF7344 0.33 -0.7 12,605 + GFF7344 0.38 +2.2 12,605 + GFF7344 0.38 +0.3 12,606 - GFF7344 0.38 -0.4 12,721 + GFF7344 0.49 -1.1 12,722 - GFF7344 0.49 +0.5 13,354 + +0.5 13,354 + +0.3 13,354 + -0.3 13,355 - -0.3 13,876 - GFF7345 0.79 -0.8 13,953 + +0.2 13,953 + -1.8 14,373 - GFF7346 0.38 -0.7 14,693 + GFF7346 0.72 -0.6 14,703 + GFF7346 0.73 +0.5 14,703 + GFF7346 0.73 +0.9 14,704 - GFF7346 0.73 -1.7 14,704 - GFF7346 0.73 -0.9
Or see this region's nucleotide sequence