Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7338 and GFF7339 overlap by 4 nucleotides GFF7339 and GFF7340 are separated by 4 nucleotides GFF7340 and GFF7341 are separated by 207 nucleotides GFF7341 and GFF7342 overlap by 4 nucleotides
GFF7338 - ABC transporter, permease protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,130 to 7,957
GFF7338
GFF7339 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,954 to 8,781
GFF7339
GFF7340 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 8,786 to 9,877
GFF7340
GFF7341 - no description, at 10,085 to 10,207
GFF7341
GFF7342 - Oxidoreductase, at 10,204 to 11,664
GFF7342
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-1
0
1 at 7.947 kb on - strand at 8.174 kb on + strand, within GFF7339 at 8.175 kb on - strand, within GFF7339 at 8.742 kb on - strand at 8.847 kb on + strand at 8.864 kb on + strand at 8.864 kb on + strand at 8.910 kb on + strand, within GFF7340 at 8.911 kb on - strand, within GFF7340 at 9.322 kb on - strand, within GFF7340 at 9.322 kb on - strand, within GFF7340 at 9.427 kb on - strand, within GFF7340 at 9.427 kb on - strand, within GFF7340 at 9.594 kb on + strand, within GFF7340 at 9.625 kb on - strand, within GFF7340 at 9.625 kb on - strand, within GFF7340 at 9.842 kb on - strand at 9.975 kb on + strand at 9.976 kb on - strand at 9.976 kb on - strand at 10.042 kb on + strand at 10.043 kb on - strand at 10.525 kb on + strand, within GFF7342 at 10.552 kb on + strand, within GFF7342 at 10.553 kb on - strand, within GFF7342
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 7,947 - -0.9 8,174 + GFF7339 0.27 +0.1 8,175 - GFF7339 0.27 -0.1 8,742 - -0.5 8,847 + -0.1 8,864 + -0.3 8,864 + +1.7 8,910 + GFF7340 0.11 -1.2 8,911 - GFF7340 0.11 -0.5 9,322 - GFF7340 0.49 -0.8 9,322 - GFF7340 0.49 -0.1 9,427 - GFF7340 0.59 +0.7 9,427 - GFF7340 0.59 -0.9 9,594 + GFF7340 0.74 -1.0 9,625 - GFF7340 0.77 -0.5 9,625 - GFF7340 0.77 +0.3 9,842 - -0.7 9,975 + -0.7 9,976 - +1.6 9,976 - -1.1 10,042 + -0.3 10,043 - +0.6 10,525 + GFF7342 0.22 +1.1 10,552 + GFF7342 0.24 -0.2 10,553 - GFF7342 0.24 +1.7
Or see this region's nucleotide sequence