Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7005 and GFF7006 overlap by 1 nucleotides GFF7006 and GFF7007 are separated by 101 nucleotides GFF7007 and GFF7008 are separated by 114 nucleotides
GFF7005 - Flagellar biosynthesis protein FlhB, at 21,691 to 22,785
GFF7005
GFF7006 - Flagellar biosynthesis protein FliR, at 22,785 to 23,501
GFF7006
GFF7007 - Flagellar biosynthesis protein FliQ, at 23,603 to 23,869
GFF7007
GFF7008 - no description, at 23,984 to 24,133
GFF7008
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 21.950 kb on + strand, within GFF7005 at 22.072 kb on - strand, within GFF7005 at 22.158 kb on - strand, within GFF7005 at 22.158 kb on - strand, within GFF7005 at 22.244 kb on + strand, within GFF7005 at 22.274 kb on + strand, within GFF7005 at 22.275 kb on - strand, within GFF7005 at 22.275 kb on - strand, within GFF7005 at 22.275 kb on - strand, within GFF7005 at 22.597 kb on + strand, within GFF7005 at 22.849 kb on + strand at 23.126 kb on - strand, within GFF7006 at 23.126 kb on - strand, within GFF7006 at 23.126 kb on - strand, within GFF7006 at 23.188 kb on + strand, within GFF7006 at 23.188 kb on + strand, within GFF7006 at 23.189 kb on - strand, within GFF7006 at 23.204 kb on + strand, within GFF7006 at 23.205 kb on - strand, within GFF7006 at 23.205 kb on - strand, within GFF7006 at 23.316 kb on + strand, within GFF7006 at 23.316 kb on + strand, within GFF7006 at 23.317 kb on - strand, within GFF7006 at 23.317 kb on - strand, within GFF7006 at 23.482 kb on + strand at 23.483 kb on - strand at 23.541 kb on + strand at 23.541 kb on + strand at 23.542 kb on - strand at 23.542 kb on - strand at 23.542 kb on - strand at 23.542 kb on - strand at 23.870 kb on + strand at 23.871 kb on - strand at 23.909 kb on + strand at 23.940 kb on + strand at 23.941 kb on - strand at 23.941 kb on - strand at 24.048 kb on + strand, within GFF7008 at 24.073 kb on - strand, within GFF7008 at 24.169 kb on + strand at 24.182 kb on - strand at 24.251 kb on - strand at 24.265 kb on + strand at 24.265 kb on + strand at 24.265 kb on + strand at 24.266 kb on - strand at 24.266 kb on - strand at 24.350 kb on + strand at 24.351 kb on - strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.384 kb on - strand at 24.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 21,950 + GFF7005 0.24 +0.5 22,072 - GFF7005 0.35 +0.0 22,158 - GFF7005 0.43 +0.0 22,158 - GFF7005 0.43 +0.5 22,244 + GFF7005 0.51 +0.0 22,274 + GFF7005 0.53 -0.8 22,275 - GFF7005 0.53 +0.9 22,275 - GFF7005 0.53 +0.5 22,275 - GFF7005 0.53 -0.0 22,597 + GFF7005 0.83 +0.2 22,849 + -0.1 23,126 - GFF7006 0.48 -0.3 23,126 - GFF7006 0.48 -1.1 23,126 - GFF7006 0.48 +0.3 23,188 + GFF7006 0.56 -0.6 23,188 + GFF7006 0.56 -1.2 23,189 - GFF7006 0.56 -0.5 23,204 + GFF7006 0.58 +1.2 23,205 - GFF7006 0.59 -0.3 23,205 - GFF7006 0.59 +0.4 23,316 + GFF7006 0.74 -2.3 23,316 + GFF7006 0.74 -1.4 23,317 - GFF7006 0.74 +0.2 23,317 - GFF7006 0.74 +1.0 23,482 + -2.6 23,483 - -1.4 23,541 + +0.2 23,541 + +0.5 23,542 - -0.4 23,542 - +0.3 23,542 - +1.5 23,542 - -0.5 23,870 + -0.5 23,871 - +0.0 23,909 + -0.3 23,940 + -0.7 23,941 - -0.3 23,941 - +0.9 24,048 + GFF7008 0.43 +0.2 24,073 - GFF7008 0.59 +1.0 24,169 + +0.6 24,182 - -0.2 24,251 - -0.1 24,265 + -0.6 24,265 + -0.7 24,265 + -0.6 24,266 - -0.7 24,266 - -2.6 24,350 + -0.4 24,351 - -2.1 24,383 + +2.3 24,383 + -0.3 24,383 + -0.7 24,383 + -0.4 24,383 + -0.8 24,384 - +0.2 24,384 - +1.1
Or see this region's nucleotide sequence