Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6981 and GFF6982 are separated by 7 nucleotides GFF6982 and GFF6983 are separated by 26 nucleotides GFF6983 and GFF6984 are separated by 34 nucleotides GFF6984 and GFF6985 are separated by 177 nucleotides
GFF6981 - hypothetical protein, at 381 to 956
GFF6981
GFF6982 - hypothetical protein, at 964 to 1,443
GFF6982
GFF6983 - FIG140336: TPR domain protein, at 1,470 to 2,747
GFF6983
GFF6984 - RNA polymerase sigma factor for flagellar operon, at 2,782 to 3,468
GFF6984
GFF6985 - Flagellar biosynthesis protein FliP, at 3,646 to 4,419
GFF6985
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.621 kb on + strand, within GFF6981 at 0.674 kb on + strand, within GFF6981 at 0.781 kb on - strand, within GFF6981 at 0.909 kb on - strand at 0.930 kb on + strand at 1.040 kb on + strand, within GFF6982 at 1.040 kb on + strand, within GFF6982 at 1.257 kb on - strand, within GFF6982 at 1.257 kb on - strand, within GFF6982 at 1.509 kb on + strand at 1.510 kb on - strand at 1.510 kb on - strand at 1.724 kb on - strand, within GFF6983 at 1.724 kb on - strand, within GFF6983 at 1.724 kb on - strand, within GFF6983 at 2.386 kb on + strand, within GFF6983 at 2.386 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.561 kb on - strand, within GFF6983 at 2.739 kb on - strand at 2.762 kb on + strand at 2.921 kb on + strand, within GFF6984 at 2.922 kb on - strand, within GFF6984 at 2.980 kb on + strand, within GFF6984 at 3.022 kb on + strand, within GFF6984 at 3.022 kb on + strand, within GFF6984 at 3.023 kb on - strand, within GFF6984 at 3.023 kb on - strand, within GFF6984 at 3.131 kb on + strand, within GFF6984 at 3.382 kb on + strand, within GFF6984 at 3.410 kb on + strand at 3.557 kb on - strand at 3.557 kb on - strand at 3.557 kb on - strand at 3.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 621 + GFF6981 0.42 +1.3 674 + GFF6981 0.51 -2.3 781 - GFF6981 0.69 -1.7 909 - +0.6 930 + +0.2 1,040 + GFF6982 0.16 +1.2 1,040 + GFF6982 0.16 +0.8 1,257 - GFF6982 0.61 -0.8 1,257 - GFF6982 0.61 -0.6 1,509 + +0.3 1,510 - -0.8 1,510 - -1.7 1,724 - GFF6983 0.20 +0.5 1,724 - GFF6983 0.20 -0.6 1,724 - GFF6983 0.20 +0.9 2,386 + GFF6983 0.72 +0.3 2,386 + GFF6983 0.72 -0.1 2,560 + GFF6983 0.85 +0.2 2,560 + GFF6983 0.85 -0.3 2,560 + GFF6983 0.85 +0.4 2,561 - GFF6983 0.85 +1.1 2,739 - -2.5 2,762 + -1.3 2,921 + GFF6984 0.20 -0.2 2,922 - GFF6984 0.20 -0.1 2,980 + GFF6984 0.29 -0.1 3,022 + GFF6984 0.35 -2.4 3,022 + GFF6984 0.35 +1.5 3,023 - GFF6984 0.35 +0.2 3,023 - GFF6984 0.35 -0.7 3,131 + GFF6984 0.51 -1.0 3,382 + GFF6984 0.87 -1.6 3,410 + -0.1 3,557 - -0.2 3,557 - -0.3 3,557 - -0.6 3,634 - -0.5
Or see this region's nucleotide sequence