Strain Fitness in Variovorax sp. SCN45 around GFF6983

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6981 and GFF6982 are separated by 7 nucleotidesGFF6982 and GFF6983 are separated by 26 nucleotidesGFF6983 and GFF6984 are separated by 34 nucleotidesGFF6984 and GFF6985 are separated by 177 nucleotides GFF6981 - hypothetical protein, at 381 to 956 GFF6981 GFF6982 - hypothetical protein, at 964 to 1,443 GFF6982 GFF6983 - FIG140336: TPR domain protein, at 1,470 to 2,747 GFF6983 GFF6984 - RNA polymerase sigma factor for flagellar operon, at 2,782 to 3,468 GFF6984 GFF6985 - Flagellar biosynthesis protein FliP, at 3,646 to 4,419 GFF6985 Position (kb) 1 2 3Strain fitness (log2 ratio) -2 -1 0 1at 0.621 kb on + strand, within GFF6981at 0.674 kb on + strand, within GFF6981at 0.781 kb on - strand, within GFF6981at 0.909 kb on - strandat 0.930 kb on + strandat 1.040 kb on + strand, within GFF6982at 1.040 kb on + strand, within GFF6982at 1.257 kb on - strand, within GFF6982at 1.257 kb on - strand, within GFF6982at 1.509 kb on + strandat 1.510 kb on - strandat 1.510 kb on - strandat 1.724 kb on - strand, within GFF6983at 1.724 kb on - strand, within GFF6983at 1.724 kb on - strand, within GFF6983at 2.386 kb on + strand, within GFF6983at 2.386 kb on + strand, within GFF6983at 2.560 kb on + strand, within GFF6983at 2.560 kb on + strand, within GFF6983at 2.560 kb on + strand, within GFF6983at 2.561 kb on - strand, within GFF6983at 2.739 kb on - strandat 2.762 kb on + strandat 2.921 kb on + strand, within GFF6984at 2.922 kb on - strand, within GFF6984at 2.980 kb on + strand, within GFF6984at 3.022 kb on + strand, within GFF6984at 3.022 kb on + strand, within GFF6984at 3.023 kb on - strand, within GFF6984at 3.023 kb on - strand, within GFF6984at 3.131 kb on + strand, within GFF6984at 3.382 kb on + strand, within GFF6984at 3.410 kb on + strandat 3.557 kb on - strandat 3.557 kb on - strandat 3.557 kb on - strandat 3.634 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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621 + GFF6981 0.42 +1.3
674 + GFF6981 0.51 -2.3
781 - GFF6981 0.69 -1.7
909 - +0.6
930 + +0.2
1,040 + GFF6982 0.16 +1.2
1,040 + GFF6982 0.16 +0.8
1,257 - GFF6982 0.61 -0.8
1,257 - GFF6982 0.61 -0.6
1,509 + +0.3
1,510 - -0.8
1,510 - -1.7
1,724 - GFF6983 0.20 +0.5
1,724 - GFF6983 0.20 -0.6
1,724 - GFF6983 0.20 +0.9
2,386 + GFF6983 0.72 +0.3
2,386 + GFF6983 0.72 -0.1
2,560 + GFF6983 0.85 +0.2
2,560 + GFF6983 0.85 -0.3
2,560 + GFF6983 0.85 +0.4
2,561 - GFF6983 0.85 +1.1
2,739 - -2.5
2,762 + -1.3
2,921 + GFF6984 0.20 -0.2
2,922 - GFF6984 0.20 -0.1
2,980 + GFF6984 0.29 -0.1
3,022 + GFF6984 0.35 -2.4
3,022 + GFF6984 0.35 +1.5
3,023 - GFF6984 0.35 +0.2
3,023 - GFF6984 0.35 -0.7
3,131 + GFF6984 0.51 -1.0
3,382 + GFF6984 0.87 -1.6
3,410 + -0.1
3,557 - -0.2
3,557 - -0.3
3,557 - -0.6
3,634 - -0.5

Or see this region's nucleotide sequence