Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6957 and GFF6958 overlap by 4 nucleotides GFF6958 and GFF6959 overlap by 4 nucleotides GFF6959 and GFF6960 are separated by 34 nucleotides GFF6960 and GFF6961 are separated by 15 nucleotides GFF6961 and GFF6962 are separated by 13 nucleotides
GFF6957 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 31,823 to 33,229
GFF6957
GFF6958 - no description, at 33,226 to 33,492
GFF6958
GFF6959 - Putative analog of CcoH, COG3198, at 33,489 to 33,770
GFF6959
GFF6960 - Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation, at 33,805 to 35,232
GFF6960
GFF6961 - Cytochrome c oxidase (cbb3-type) subunit CcoP (EC 1.9.3.1), at 35,248 to 36,165
GFF6961
GFF6962 - Cytochrome c oxidase (cbb3-type) subunit CcoQ (EC 1.9.3.1), at 36,179 to 36,319
GFF6962
Position (kb)
33
34
35
36 Strain fitness (log2 ratio)
-1
0
1
2 at 32.931 kb on + strand, within GFF6957 at 32.931 kb on + strand, within GFF6957 at 32.931 kb on + strand, within GFF6957 at 32.991 kb on + strand, within GFF6957 at 32.991 kb on + strand, within GFF6957 at 32.991 kb on + strand, within GFF6957 at 32.991 kb on + strand, within GFF6957 at 32.994 kb on + strand, within GFF6957 at 33.027 kb on + strand, within GFF6957 at 33.027 kb on + strand, within GFF6957 at 33.028 kb on - strand, within GFF6957 at 33.028 kb on - strand, within GFF6957 at 33.028 kb on - strand, within GFF6957 at 33.132 kb on + strand at 33.132 kb on + strand at 33.141 kb on + strand at 33.347 kb on + strand, within GFF6958 at 33.347 kb on + strand, within GFF6958 at 33.347 kb on + strand, within GFF6958 at 33.347 kb on + strand, within GFF6958 at 33.348 kb on - strand, within GFF6958 at 33.348 kb on - strand, within GFF6958 at 33.348 kb on - strand, within GFF6958 at 33.619 kb on + strand, within GFF6959 at 33.620 kb on - strand, within GFF6959 at 34.017 kb on - strand, within GFF6960 at 34.017 kb on - strand, within GFF6960 at 34.059 kb on - strand, within GFF6960 at 34.296 kb on - strand, within GFF6960 at 34.359 kb on - strand, within GFF6960 at 34.499 kb on + strand, within GFF6960 at 34.500 kb on - strand, within GFF6960 at 34.551 kb on - strand, within GFF6960 at 34.562 kb on + strand, within GFF6960 at 34.563 kb on - strand, within GFF6960 at 34.563 kb on - strand, within GFF6960 at 34.862 kb on + strand, within GFF6960 at 34.883 kb on + strand, within GFF6960 at 34.884 kb on - strand, within GFF6960 at 34.961 kb on + strand, within GFF6960 at 34.962 kb on - strand, within GFF6960 at 34.962 kb on - strand, within GFF6960 at 35.000 kb on + strand, within GFF6960 at 35.000 kb on + strand, within GFF6960 at 35.000 kb on + strand, within GFF6960 at 35.553 kb on - strand, within GFF6961 at 35.553 kb on - strand, within GFF6961 at 35.667 kb on - strand, within GFF6961 at 35.816 kb on + strand, within GFF6961 at 35.871 kb on - strand, within GFF6961 at 36.131 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 32,931 + GFF6957 0.79 -0.8 32,931 + GFF6957 0.79 -1.2 32,931 + GFF6957 0.79 -0.4 32,991 + GFF6957 0.83 -0.3 32,991 + GFF6957 0.83 -1.0 32,991 + GFF6957 0.83 -0.6 32,991 + GFF6957 0.83 +0.2 32,994 + GFF6957 0.83 -0.7 33,027 + GFF6957 0.86 -0.1 33,027 + GFF6957 0.86 -0.9 33,028 - GFF6957 0.86 -0.2 33,028 - GFF6957 0.86 -0.1 33,028 - GFF6957 0.86 +0.1 33,132 + +0.1 33,132 + +1.5 33,141 + -0.3 33,347 + GFF6958 0.45 +0.1 33,347 + GFF6958 0.45 -0.1 33,347 + GFF6958 0.45 -0.4 33,347 + GFF6958 0.45 +0.6 33,348 - GFF6958 0.46 +0.9 33,348 - GFF6958 0.46 -0.6 33,348 - GFF6958 0.46 +0.0 33,619 + GFF6959 0.46 +1.4 33,620 - GFF6959 0.46 -0.8 34,017 - GFF6960 0.15 -0.1 34,017 - GFF6960 0.15 +1.7 34,059 - GFF6960 0.18 -0.8 34,296 - GFF6960 0.34 -0.5 34,359 - GFF6960 0.39 +1.8 34,499 + GFF6960 0.49 +0.1 34,500 - GFF6960 0.49 -0.5 34,551 - GFF6960 0.52 +0.6 34,562 + GFF6960 0.53 -1.0 34,563 - GFF6960 0.53 -1.3 34,563 - GFF6960 0.53 -0.6 34,862 + GFF6960 0.74 +1.1 34,883 + GFF6960 0.75 -0.0 34,884 - GFF6960 0.76 +0.4 34,961 + GFF6960 0.81 +0.8 34,962 - GFF6960 0.81 -0.9 34,962 - GFF6960 0.81 +0.1 35,000 + GFF6960 0.84 +0.5 35,000 + GFF6960 0.84 +0.1 35,000 + GFF6960 0.84 -0.2 35,553 - GFF6961 0.33 +0.6 35,553 - GFF6961 0.33 +1.2 35,667 - GFF6961 0.46 +1.1 35,816 + GFF6961 0.62 -1.0 35,871 - GFF6961 0.68 -0.6 36,131 + +0.4
Or see this region's nucleotide sequence