Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6803 and GFF6804 are separated by 30 nucleotides GFF6804 and GFF6805 overlap by 4 nucleotides GFF6805 and GFF6806 are separated by 142 nucleotides GFF6806 and GFF6807 are separated by 16 nucleotides
GFF6803 - N-acyl homoserine lactone hydrolase, at 50,144 to 51,217
GFF6803
GFF6804 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 51,248 to 52,021
GFF6804
GFF6805 - FIG00821908: hypothetical protein, at 52,018 to 52,524
GFF6805
GFF6806 - transcriptional regulator, Crp/Fnr family, at 52,667 to 53,410
GFF6806
GFF6807 - BUG/TctC family periplasmic protein, at 53,427 to 54,392
GFF6807
Position (kb) 52
53 Strain fitness (log2 ratio) -2
-1
0
1 at 51.252 kb on + strand at 51.543 kb on + strand, within GFF6804 at 51.700 kb on + strand, within GFF6804 at 51.700 kb on + strand, within GFF6804 at 51.700 kb on + strand, within GFF6804 at 51.701 kb on - strand, within GFF6804 at 51.701 kb on - strand, within GFF6804 at 52.145 kb on + strand, within GFF6805 at 52.352 kb on + strand, within GFF6805 at 52.352 kb on + strand, within GFF6805 at 52.353 kb on - strand, within GFF6805 at 52.353 kb on - strand, within GFF6805 at 52.353 kb on - strand, within GFF6805 at 52.355 kb on + strand, within GFF6805 at 52.409 kb on + strand, within GFF6805 at 52.410 kb on - strand, within GFF6805 at 52.600 kb on + strand at 52.600 kb on + strand at 52.601 kb on - strand at 53.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 51,252 + -0.4 51,543 + GFF6804 0.38 -0.2 51,700 + GFF6804 0.58 -0.3 51,700 + GFF6804 0.58 -0.6 51,700 + GFF6804 0.58 +0.3 51,701 - GFF6804 0.59 -0.9 51,701 - GFF6804 0.59 +1.6 52,145 + GFF6805 0.25 -1.5 52,352 + GFF6805 0.66 -0.4 52,352 + GFF6805 0.66 +0.6 52,353 - GFF6805 0.66 -1.4 52,353 - GFF6805 0.66 -2.0 52,353 - GFF6805 0.66 -1.5 52,355 + GFF6805 0.66 +0.0 52,409 + GFF6805 0.77 -0.1 52,410 - GFF6805 0.77 +1.3 52,600 + +0.8 52,600 + -0.4 52,601 - -0.9 53,518 + -0.6
Or see this region's nucleotide sequence