Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6716 and GFF6717 are separated by 150 nucleotides GFF6717 and GFF6718 are separated by 106 nucleotides GFF6718 and GFF6719 are separated by 7 nucleotides
GFF6716 - N-acyl-D-amino-acid deacylase (EC 3.5.1.81), at 24,520 to 26,001
GFF6716
GFF6717 - hypothetical protein, at 26,152 to 28,392
GFF6717
GFF6718 - no description, at 28,499 to 28,918
GFF6718
GFF6719 - hypothetical protein, at 28,926 to 29,489
GFF6719
Position (kb)
26
27
28
29 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 25.192 kb on + strand, within GFF6716 at 25.390 kb on + strand, within GFF6716 at 25.390 kb on + strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.396 kb on + strand, within GFF6716 at 25.574 kb on - strand, within GFF6716 at 25.870 kb on + strand at 25.870 kb on + strand at 25.870 kb on + strand at 25.870 kb on + strand at 26.043 kb on + strand at 26.069 kb on + strand at 26.163 kb on + strand at 26.253 kb on + strand at 26.253 kb on + strand at 26.254 kb on - strand at 26.254 kb on - strand at 26.357 kb on - strand at 26.479 kb on + strand, within GFF6717 at 26.725 kb on + strand, within GFF6717 at 26.725 kb on + strand, within GFF6717 at 26.726 kb on - strand, within GFF6717 at 26.752 kb on + strand, within GFF6717 at 26.752 kb on + strand, within GFF6717 at 26.753 kb on - strand, within GFF6717 at 26.828 kb on + strand, within GFF6717 at 26.829 kb on - strand, within GFF6717 at 26.829 kb on - strand, within GFF6717 at 26.852 kb on - strand, within GFF6717 at 26.914 kb on + strand, within GFF6717 at 26.914 kb on + strand, within GFF6717 at 27.358 kb on + strand, within GFF6717 at 27.359 kb on - strand, within GFF6717 at 27.359 kb on - strand, within GFF6717 at 27.359 kb on - strand, within GFF6717 at 27.362 kb on - strand, within GFF6717 at 27.362 kb on - strand, within GFF6717 at 27.362 kb on - strand, within GFF6717 at 27.403 kb on - strand, within GFF6717 at 27.448 kb on - strand, within GFF6717 at 27.448 kb on - strand, within GFF6717 at 27.451 kb on + strand, within GFF6717 at 27.452 kb on - strand, within GFF6717 at 27.538 kb on + strand, within GFF6717 at 27.539 kb on - strand, within GFF6717 at 27.541 kb on + strand, within GFF6717 at 27.541 kb on + strand, within GFF6717 at 27.541 kb on + strand, within GFF6717 at 27.542 kb on - strand, within GFF6717 at 27.542 kb on - strand, within GFF6717 at 27.684 kb on + strand, within GFF6717 at 27.685 kb on - strand, within GFF6717 at 27.685 kb on - strand, within GFF6717 at 27.685 kb on - strand, within GFF6717 at 27.685 kb on - strand, within GFF6717 at 27.760 kb on + strand, within GFF6717 at 27.761 kb on - strand, within GFF6717 at 27.852 kb on + strand, within GFF6717 at 27.852 kb on + strand, within GFF6717 at 27.853 kb on - strand, within GFF6717 at 27.853 kb on - strand, within GFF6717 at 28.015 kb on + strand, within GFF6717 at 28.015 kb on + strand, within GFF6717 at 28.016 kb on - strand, within GFF6717 at 28.047 kb on + strand, within GFF6717 at 28.047 kb on + strand, within GFF6717 at 28.102 kb on + strand, within GFF6717 at 28.103 kb on - strand, within GFF6717 at 28.118 kb on + strand, within GFF6717 at 28.153 kb on + strand, within GFF6717 at 28.177 kb on + strand at 28.177 kb on + strand at 28.177 kb on + strand at 28.177 kb on + strand at 28.177 kb on + strand at 28.177 kb on + strand at 28.177 kb on + strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.178 kb on - strand at 28.179 kb on + strand at 28.179 kb on + strand at 28.179 kb on + strand at 28.180 kb on - strand at 28.288 kb on + strand at 28.315 kb on + strand at 28.315 kb on + strand at 28.316 kb on - strand at 28.316 kb on - strand at 28.575 kb on - strand, within GFF6718 at 28.609 kb on + strand, within GFF6718 at 28.839 kb on - strand, within GFF6718 at 29.070 kb on - strand, within GFF6719 at 29.079 kb on + strand, within GFF6719 at 29.169 kb on + strand, within GFF6719 at 29.169 kb on + strand, within GFF6719 at 29.169 kb on + strand, within GFF6719 at 29.169 kb on + strand, within GFF6719 at 29.169 kb on + strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.170 kb on - strand, within GFF6719 at 29.220 kb on + strand, within GFF6719 at 29.221 kb on - strand, within GFF6719 at 29.221 kb on - strand, within GFF6719 at 29.221 kb on - strand, within GFF6719 at 29.221 kb on - strand, within GFF6719 at 29.221 kb on - strand, within GFF6719 at 29.334 kb on + strand, within GFF6719 at 29.335 kb on - strand, within GFF6719 at 29.335 kb on - strand, within GFF6719 at 29.369 kb on + strand, within GFF6719 at 29.370 kb on - strand, within GFF6719
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 25,192 + GFF6716 0.45 -0.8 25,390 + GFF6716 0.59 +1.0 25,390 + GFF6716 0.59 +0.1 25,391 - GFF6716 0.59 +0.6 25,391 - GFF6716 0.59 +0.4 25,391 - GFF6716 0.59 -1.9 25,391 - GFF6716 0.59 -0.4 25,396 + GFF6716 0.59 +1.8 25,574 - GFF6716 0.71 -0.4 25,870 + -1.3 25,870 + -0.1 25,870 + +0.5 25,870 + -0.2 26,043 + -0.6 26,069 + +1.0 26,163 + -0.8 26,253 + -0.1 26,253 + -0.5 26,254 - -0.7 26,254 - +0.9 26,357 - +0.4 26,479 + GFF6717 0.15 -0.4 26,725 + GFF6717 0.26 -0.5 26,725 + GFF6717 0.26 +1.6 26,726 - GFF6717 0.26 -0.6 26,752 + GFF6717 0.27 -0.7 26,752 + GFF6717 0.27 -0.2 26,753 - GFF6717 0.27 -1.9 26,828 + GFF6717 0.30 -0.6 26,829 - GFF6717 0.30 -1.1 26,829 - GFF6717 0.30 -0.8 26,852 - GFF6717 0.31 -0.5 26,914 + GFF6717 0.34 -0.4 26,914 + GFF6717 0.34 +0.9 27,358 + GFF6717 0.54 -1.0 27,359 - GFF6717 0.54 -1.6 27,359 - GFF6717 0.54 -0.5 27,359 - GFF6717 0.54 -0.1 27,362 - GFF6717 0.54 -0.7 27,362 - GFF6717 0.54 -1.2 27,362 - GFF6717 0.54 -0.1 27,403 - GFF6717 0.56 -0.0 27,448 - GFF6717 0.58 +0.2 27,448 - GFF6717 0.58 -0.6 27,451 + GFF6717 0.58 +0.4 27,452 - GFF6717 0.58 +0.6 27,538 + GFF6717 0.62 +0.9 27,539 - GFF6717 0.62 +0.3 27,541 + GFF6717 0.62 +0.3 27,541 + GFF6717 0.62 -0.0 27,541 + GFF6717 0.62 -0.0 27,542 - GFF6717 0.62 -0.2 27,542 - GFF6717 0.62 +0.2 27,684 + GFF6717 0.68 -0.6 27,685 - GFF6717 0.68 +0.9 27,685 - GFF6717 0.68 -1.0 27,685 - GFF6717 0.68 -0.2 27,685 - GFF6717 0.68 -0.4 27,760 + GFF6717 0.72 +0.4 27,761 - GFF6717 0.72 +0.3 27,852 + GFF6717 0.76 -0.5 27,852 + GFF6717 0.76 -0.4 27,853 - GFF6717 0.76 -0.1 27,853 - GFF6717 0.76 +0.1 28,015 + GFF6717 0.83 -0.2 28,015 + GFF6717 0.83 -2.0 28,016 - GFF6717 0.83 -0.3 28,047 + GFF6717 0.85 +0.9 28,047 + GFF6717 0.85 -1.1 28,102 + GFF6717 0.87 -0.1 28,103 - GFF6717 0.87 +0.1 28,118 + GFF6717 0.88 -0.0 28,153 + GFF6717 0.89 -0.9 28,177 + -0.2 28,177 + -0.3 28,177 + -1.4 28,177 + +1.3 28,177 + +1.2 28,177 + -0.2 28,177 + +0.1 28,178 - -0.5 28,178 - +1.1 28,178 - -0.6 28,178 - -0.0 28,178 - -0.5 28,178 - -0.1 28,178 - +0.4 28,178 - -0.1 28,178 - -0.3 28,178 - -0.6 28,179 + -0.7 28,179 + +0.3 28,179 + +0.3 28,180 - +0.2 28,288 + -0.8 28,315 + -0.1 28,315 + +0.3 28,316 - -0.3 28,316 - -1.2 28,575 - GFF6718 0.18 +1.3 28,609 + GFF6718 0.26 -0.3 28,839 - GFF6718 0.81 +1.0 29,070 - GFF6719 0.26 +0.2 29,079 + GFF6719 0.27 +0.5 29,169 + GFF6719 0.43 -1.2 29,169 + GFF6719 0.43 +0.4 29,169 + GFF6719 0.43 -0.3 29,169 + GFF6719 0.43 +0.9 29,169 + GFF6719 0.43 +0.8 29,170 - GFF6719 0.43 +0.2 29,170 - GFF6719 0.43 -1.5 29,170 - GFF6719 0.43 +0.6 29,170 - GFF6719 0.43 +1.2 29,170 - GFF6719 0.43 -0.5 29,170 - GFF6719 0.43 -0.2 29,170 - GFF6719 0.43 -0.9 29,170 - GFF6719 0.43 +0.4 29,220 + GFF6719 0.52 -0.4 29,221 - GFF6719 0.52 +0.6 29,221 - GFF6719 0.52 -0.1 29,221 - GFF6719 0.52 -0.3 29,221 - GFF6719 0.52 -1.0 29,221 - GFF6719 0.52 +1.0 29,334 + GFF6719 0.72 -1.7 29,335 - GFF6719 0.73 -2.5 29,335 - GFF6719 0.73 -0.9 29,369 + GFF6719 0.79 -0.7 29,370 - GFF6719 0.79 -1.6
Or see this region's nucleotide sequence