Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6460 and GFF6461 are separated by 14 nucleotides GFF6461 and GFF6462 overlap by 4 nucleotides GFF6462 and GFF6463 are separated by 11 nucleotides GFF6463 and GFF6464 are separated by 141 nucleotides
GFF6460 - Sensor protein BasS/PmrB (activates BasR/PmrA), at 37,377 to 38,732
GFF6460
GFF6461 - Thioesterase superfamily, at 38,747 to 39,169
GFF6461
GFF6462 - Integral membrane protein, at 39,166 to 40,065
GFF6462
GFF6463 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 40,077 to 40,859
GFF6463
GFF6464 - Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1), at 41,001 to 42,698
GFF6464
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 38.916 kb on + strand, within GFF6461 at 38.916 kb on + strand, within GFF6461 at 38.916 kb on + strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.917 kb on - strand, within GFF6461 at 38.943 kb on + strand, within GFF6461 at 39.066 kb on + strand, within GFF6461 at 39.066 kb on + strand, within GFF6461 at 39.066 kb on + strand, within GFF6461 at 39.067 kb on - strand, within GFF6461 at 39.067 kb on - strand, within GFF6461 at 39.067 kb on - strand, within GFF6461 at 39.132 kb on + strand at 39.133 kb on - strand at 39.133 kb on - strand at 39.266 kb on + strand, within GFF6462 at 39.266 kb on + strand, within GFF6462 at 39.266 kb on + strand, within GFF6462 at 39.266 kb on + strand, within GFF6462 at 39.266 kb on + strand, within GFF6462 at 39.267 kb on - strand, within GFF6462 at 39.267 kb on - strand, within GFF6462 at 39.563 kb on + strand, within GFF6462 at 39.564 kb on - strand, within GFF6462 at 39.564 kb on - strand, within GFF6462 at 39.564 kb on - strand, within GFF6462 at 39.564 kb on - strand, within GFF6462 at 39.833 kb on + strand, within GFF6462 at 39.833 kb on + strand, within GFF6462 at 39.833 kb on + strand, within GFF6462 at 39.834 kb on - strand, within GFF6462 at 39.834 kb on - strand, within GFF6462 at 39.834 kb on - strand, within GFF6462 at 39.836 kb on + strand, within GFF6462 at 39.837 kb on - strand, within GFF6462 at 40.264 kb on + strand, within GFF6463 at 40.264 kb on + strand, within GFF6463 at 40.360 kb on + strand, within GFF6463 at 40.360 kb on + strand, within GFF6463 at 40.361 kb on - strand, within GFF6463 at 40.361 kb on - strand, within GFF6463 at 40.361 kb on - strand, within GFF6463 at 40.361 kb on - strand, within GFF6463 at 40.361 kb on - strand, within GFF6463 at 40.361 kb on - strand, within GFF6463 at 40.407 kb on - strand, within GFF6463 at 40.407 kb on - strand, within GFF6463 at 40.429 kb on + strand, within GFF6463 at 40.429 kb on + strand, within GFF6463 at 40.429 kb on + strand, within GFF6463 at 40.429 kb on + strand, within GFF6463 at 40.429 kb on + strand, within GFF6463 at 40.429 kb on + strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.430 kb on - strand, within GFF6463 at 40.525 kb on + strand, within GFF6463 at 40.526 kb on - strand, within GFF6463 at 40.526 kb on - strand, within GFF6463 at 40.526 kb on - strand, within GFF6463 at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.940 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 38,916 + GFF6461 0.40 -2.4 38,916 + GFF6461 0.40 -0.0 38,916 + GFF6461 0.40 -1.1 38,917 - GFF6461 0.40 -1.3 38,917 - GFF6461 0.40 -0.3 38,917 - GFF6461 0.40 +0.4 38,917 - GFF6461 0.40 -0.5 38,917 - GFF6461 0.40 -0.6 38,917 - GFF6461 0.40 -1.6 38,917 - GFF6461 0.40 -0.2 38,917 - GFF6461 0.40 +0.1 38,917 - GFF6461 0.40 +0.0 38,917 - GFF6461 0.40 -0.0 38,917 - GFF6461 0.40 +1.6 38,917 - GFF6461 0.40 +0.5 38,917 - GFF6461 0.40 -0.9 38,917 - GFF6461 0.40 +1.1 38,917 - GFF6461 0.40 +0.0 38,917 - GFF6461 0.40 -0.2 38,943 + GFF6461 0.46 +0.9 39,066 + GFF6461 0.75 -0.7 39,066 + GFF6461 0.75 -0.9 39,066 + GFF6461 0.75 -1.1 39,067 - GFF6461 0.76 -0.5 39,067 - GFF6461 0.76 +0.1 39,067 - GFF6461 0.76 +1.2 39,132 + +0.6 39,133 - +0.4 39,133 - -0.0 39,266 + GFF6462 0.11 -1.0 39,266 + GFF6462 0.11 -0.1 39,266 + GFF6462 0.11 -0.7 39,266 + GFF6462 0.11 -1.2 39,266 + GFF6462 0.11 -0.5 39,267 - GFF6462 0.11 -0.4 39,267 - GFF6462 0.11 -0.3 39,563 + GFF6462 0.44 -0.4 39,564 - GFF6462 0.44 -1.2 39,564 - GFF6462 0.44 -1.4 39,564 - GFF6462 0.44 -0.3 39,564 - GFF6462 0.44 +0.5 39,833 + GFF6462 0.74 -1.6 39,833 + GFF6462 0.74 -1.2 39,833 + GFF6462 0.74 +0.1 39,834 - GFF6462 0.74 -0.8 39,834 - GFF6462 0.74 -0.4 39,834 - GFF6462 0.74 -1.4 39,836 + GFF6462 0.74 -0.5 39,837 - GFF6462 0.75 -1.9 40,264 + GFF6463 0.24 -0.3 40,264 + GFF6463 0.24 -1.4 40,360 + GFF6463 0.36 +0.5 40,360 + GFF6463 0.36 +0.0 40,361 - GFF6463 0.36 +1.0 40,361 - GFF6463 0.36 +0.4 40,361 - GFF6463 0.36 +0.4 40,361 - GFF6463 0.36 +0.9 40,361 - GFF6463 0.36 +0.3 40,361 - GFF6463 0.36 -1.6 40,407 - GFF6463 0.42 -0.6 40,407 - GFF6463 0.42 +0.8 40,429 + GFF6463 0.45 +0.6 40,429 + GFF6463 0.45 -0.9 40,429 + GFF6463 0.45 +0.2 40,429 + GFF6463 0.45 -0.7 40,429 + GFF6463 0.45 -0.6 40,429 + GFF6463 0.45 +1.3 40,430 - GFF6463 0.45 -0.5 40,430 - GFF6463 0.45 +0.2 40,430 - GFF6463 0.45 +0.3 40,430 - GFF6463 0.45 -0.1 40,430 - GFF6463 0.45 -0.1 40,430 - GFF6463 0.45 -1.2 40,430 - GFF6463 0.45 -0.6 40,430 - GFF6463 0.45 -2.1 40,525 + GFF6463 0.57 +0.6 40,526 - GFF6463 0.57 +0.8 40,526 - GFF6463 0.57 +0.1 40,526 - GFF6463 0.57 +0.8 40,852 + +0.4 40,852 + -0.5 40,852 + -0.0 40,852 + -0.7 40,852 + -1.7 40,852 + -0.0 40,852 + -0.5 40,852 + -1.6 40,852 + +0.3 40,852 + +0.1 40,852 + +0.3 40,853 - -0.1 40,853 - +0.5 40,853 - +3.9 40,853 - -1.9 40,853 - +0.4 40,940 + -0.8
Or see this region's nucleotide sequence