Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6347 and GFF6348 are separated by 107 nucleotides GFF6348 and GFF6349 are separated by 36 nucleotides GFF6349 and GFF6350 are separated by 78 nucleotides
GFF6347 - AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination, at 9,530 to 10,933
GFF6347
GFF6348 - no description, at 11,041 to 11,322
GFF6348
GFF6349 - no description, at 11,359 to 11,658
GFF6349
GFF6350 - T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA, at 11,737 to 13,650
GFF6350
Position (kb)
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 10.700 kb on + strand, within GFF6347 at 10.701 kb on - strand, within GFF6347 at 10.904 kb on + strand at 10.972 kb on + strand at 10.972 kb on + strand at 10.973 kb on - strand at 10.973 kb on - strand at 10.973 kb on - strand at 10.973 kb on - strand at 10.973 kb on - strand at 10.973 kb on - strand at 11.113 kb on + strand, within GFF6348 at 11.113 kb on + strand, within GFF6348 at 11.113 kb on + strand, within GFF6348 at 11.113 kb on + strand, within GFF6348 at 11.113 kb on + strand, within GFF6348 at 11.113 kb on + strand, within GFF6348 at 11.114 kb on - strand, within GFF6348 at 11.114 kb on - strand, within GFF6348 at 11.114 kb on - strand, within GFF6348 at 11.114 kb on - strand, within GFF6348 at 11.114 kb on - strand, within GFF6348 at 11.141 kb on + strand, within GFF6348 at 11.142 kb on - strand, within GFF6348 at 11.142 kb on - strand, within GFF6348 at 11.172 kb on - strand, within GFF6348 at 11.251 kb on + strand, within GFF6348 at 11.251 kb on + strand, within GFF6348 at 11.251 kb on + strand, within GFF6348 at 11.251 kb on + strand, within GFF6348 at 11.252 kb on - strand, within GFF6348 at 11.252 kb on - strand, within GFF6348 at 11.252 kb on - strand, within GFF6348 at 11.252 kb on - strand, within GFF6348 at 11.252 kb on - strand, within GFF6348 at 11.299 kb on + strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.320 kb on + strand at 11.321 kb on - strand at 11.405 kb on - strand, within GFF6349 at 11.515 kb on + strand, within GFF6349 at 11.515 kb on + strand, within GFF6349 at 11.516 kb on - strand, within GFF6349 at 11.559 kb on - strand, within GFF6349 at 11.559 kb on - strand, within GFF6349 at 11.671 kb on - strand at 11.671 kb on - strand at 11.738 kb on + strand at 11.738 kb on + strand at 11.739 kb on - strand at 11.801 kb on + strand at 11.802 kb on - strand at 11.837 kb on + strand at 11.837 kb on + strand at 11.837 kb on + strand at 11.837 kb on + strand at 11.838 kb on - strand at 11.838 kb on - strand at 11.838 kb on - strand at 11.838 kb on - strand at 12.068 kb on + strand, within GFF6350 at 12.069 kb on - strand, within GFF6350 at 12.069 kb on - strand, within GFF6350 at 12.077 kb on + strand, within GFF6350 at 12.077 kb on + strand, within GFF6350 at 12.378 kb on + strand, within GFF6350
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 10,700 + GFF6347 0.83 -0.6 10,701 - GFF6347 0.83 -0.5 10,904 + +1.6 10,972 + -1.3 10,972 + +0.6 10,973 - -0.5 10,973 - +0.0 10,973 - +0.1 10,973 - +1.0 10,973 - -0.2 10,973 - -0.5 11,113 + GFF6348 0.26 -0.0 11,113 + GFF6348 0.26 +0.0 11,113 + GFF6348 0.26 +2.0 11,113 + GFF6348 0.26 +0.5 11,113 + GFF6348 0.26 -2.5 11,113 + GFF6348 0.26 +0.0 11,114 - GFF6348 0.26 -0.1 11,114 - GFF6348 0.26 -1.5 11,114 - GFF6348 0.26 +2.0 11,114 - GFF6348 0.26 +0.5 11,114 - GFF6348 0.26 +0.7 11,141 + GFF6348 0.35 -1.7 11,142 - GFF6348 0.36 +0.4 11,142 - GFF6348 0.36 -2.1 11,172 - GFF6348 0.46 -0.0 11,251 + GFF6348 0.74 -2.7 11,251 + GFF6348 0.74 +0.7 11,251 + GFF6348 0.74 -0.0 11,251 + GFF6348 0.74 -1.2 11,252 - GFF6348 0.75 +0.1 11,252 - GFF6348 0.75 +0.8 11,252 - GFF6348 0.75 -0.4 11,252 - GFF6348 0.75 +0.2 11,252 - GFF6348 0.75 -0.1 11,299 + -0.2 11,300 - -0.4 11,300 - -0.9 11,320 + -0.6 11,321 - -0.5 11,405 - GFF6349 0.15 +0.2 11,515 + GFF6349 0.52 -0.6 11,515 + GFF6349 0.52 -1.0 11,516 - GFF6349 0.52 -1.4 11,559 - GFF6349 0.67 -1.0 11,559 - GFF6349 0.67 -1.0 11,671 - +0.2 11,671 - -0.1 11,738 + +2.3 11,738 + -1.0 11,739 - -0.4 11,801 + +0.6 11,802 - +1.0 11,837 + -0.5 11,837 + -0.7 11,837 + -0.4 11,837 + +0.3 11,838 - +0.1 11,838 - +2.7 11,838 - +1.4 11,838 - -1.5 12,068 + GFF6350 0.17 -1.4 12,069 - GFF6350 0.17 -0.9 12,069 - GFF6350 0.17 +2.3 12,077 + GFF6350 0.18 +0.5 12,077 + GFF6350 0.18 +0.2 12,378 + GFF6350 0.33 -0.0
Or see this region's nucleotide sequence