Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6232 and GFF6233 are separated by 2 nucleotides GFF6233 and GFF6234 are separated by 1 nucleotides GFF6234 and GFF6235 overlap by 4 nucleotides
GFF6232 - hypothetical protein, at 16,283 to 21,721
GFF6232
GFF6233 - hypothetical protein, at 21,724 to 23,433
GFF6233
GFF6234 - hypothetical protein, at 23,435 to 23,584
GFF6234
GFF6235 - hypothetical protein, at 23,581 to 25,701
GFF6235
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 21.750 kb on - strand at 21.899 kb on + strand, within GFF6233 at 21.900 kb on - strand, within GFF6233 at 22.121 kb on + strand, within GFF6233 at 22.121 kb on + strand, within GFF6233 at 22.122 kb on - strand, within GFF6233 at 22.122 kb on - strand, within GFF6233 at 22.454 kb on + strand, within GFF6233 at 22.526 kb on + strand, within GFF6233 at 22.526 kb on + strand, within GFF6233 at 22.586 kb on + strand, within GFF6233 at 22.587 kb on - strand, within GFF6233 at 22.698 kb on - strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.722 kb on - strand, within GFF6233 at 23.133 kb on - strand, within GFF6233 at 23.133 kb on - strand, within GFF6233 at 23.407 kb on - strand at 23.483 kb on - strand, within GFF6234 at 23.601 kb on + strand at 23.601 kb on + strand at 23.602 kb on - strand at 23.704 kb on + strand at 23.704 kb on + strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.984 kb on + strand, within GFF6235 at 23.985 kb on - strand, within GFF6235 at 23.985 kb on - strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.097 kb on - strand, within GFF6235 at 24.097 kb on - strand, within GFF6235 at 24.155 kb on + strand, within GFF6235 at 24.155 kb on + strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.282 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 21,750 - +2.0 21,899 + GFF6233 0.10 -0.0 21,900 - GFF6233 0.10 +0.5 22,121 + GFF6233 0.23 +0.0 22,121 + GFF6233 0.23 -0.4 22,122 - GFF6233 0.23 -0.5 22,122 - GFF6233 0.23 +0.8 22,454 + GFF6233 0.43 -0.1 22,526 + GFF6233 0.47 +3.0 22,526 + GFF6233 0.47 -1.4 22,586 + GFF6233 0.50 -3.0 22,587 - GFF6233 0.50 -0.3 22,698 - GFF6233 0.57 +0.6 22,721 + GFF6233 0.58 +0.2 22,721 + GFF6233 0.58 +0.5 22,722 - GFF6233 0.58 +0.1 23,133 - GFF6233 0.82 +1.0 23,133 - GFF6233 0.82 -0.0 23,407 - +1.5 23,483 - GFF6234 0.32 -0.1 23,601 + -0.1 23,601 + -0.1 23,602 - -0.0 23,704 + -1.1 23,704 + +0.1 23,705 - -0.7 23,705 - -1.4 23,705 - +0.6 23,705 - -0.2 23,705 - +0.5 23,984 + GFF6235 0.19 +0.0 23,985 - GFF6235 0.19 +0.3 23,985 - GFF6235 0.19 -0.4 24,089 + GFF6235 0.24 -0.6 24,089 + GFF6235 0.24 +1.8 24,089 + GFF6235 0.24 -0.9 24,089 + GFF6235 0.24 +0.9 24,089 + GFF6235 0.24 +0.4 24,089 + GFF6235 0.24 -0.1 24,089 + GFF6235 0.24 +0.4 24,089 + GFF6235 0.24 +0.5 24,090 - GFF6235 0.24 +0.6 24,090 - GFF6235 0.24 -0.5 24,090 - GFF6235 0.24 -0.1 24,090 - GFF6235 0.24 -0.4 24,090 - GFF6235 0.24 -0.0 24,097 - GFF6235 0.24 +0.6 24,097 - GFF6235 0.24 -0.9 24,155 + GFF6235 0.27 -0.2 24,155 + GFF6235 0.27 -0.1 24,156 - GFF6235 0.27 -0.3 24,156 - GFF6235 0.27 +0.3 24,156 - GFF6235 0.27 -0.7 24,282 - GFF6235 0.33 -0.1 24,318 - GFF6235 0.35 +1.3 24,318 - GFF6235 0.35 -0.8 24,318 - GFF6235 0.35 +0.2
Or see this region's nucleotide sequence