Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6162 and GFF6163 are separated by 11 nucleotides GFF6163 and GFF6164 are separated by 4 nucleotides GFF6164 and GFF6165 overlap by 4 nucleotides GFF6165 and GFF6166 are separated by 49 nucleotides
GFF6162 - Dihydrodipicolinate synthase family protein RB7999, at 67,753 to 68,820
GFF6162
GFF6163 - hypothetical protein, at 68,832 to 69,686
GFF6163
GFF6164 - FIG01094124: hypothetical protein, at 69,691 to 70,740
GFF6164
GFF6165 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 70,737 to 71,603
GFF6165
GFF6166 - Transcriptional regulator, AcrR family, at 71,653 to 72,312
GFF6166
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 69.343 kb on + strand, within GFF6163 at 69.343 kb on + strand, within GFF6163 at 69.343 kb on + strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.695 kb on + strand at 69.695 kb on + strand at 69.695 kb on + strand at 69.696 kb on - strand at 69.696 kb on - strand at 69.734 kb on + strand at 69.735 kb on - strand at 69.735 kb on - strand at 69.890 kb on + strand, within GFF6164 at 69.890 kb on + strand, within GFF6164 at 69.890 kb on + strand, within GFF6164 at 69.891 kb on - strand, within GFF6164 at 69.891 kb on - strand, within GFF6164 at 70.115 kb on + strand, within GFF6164 at 70.115 kb on + strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.163 kb on + strand, within GFF6164 at 70.163 kb on + strand, within GFF6164 at 70.164 kb on - strand, within GFF6164 at 70.203 kb on - strand, within GFF6164 at 70.203 kb on - strand, within GFF6164 at 70.268 kb on + strand, within GFF6164 at 70.668 kb on - strand at 70.668 kb on - strand at 70.796 kb on - strand at 70.796 kb on - strand at 70.972 kb on + strand, within GFF6165 at 70.972 kb on + strand, within GFF6165 at 71.146 kb on + strand, within GFF6165 at 71.147 kb on - strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.345 kb on - strand, within GFF6165 at 71.345 kb on - strand, within GFF6165 at 71.567 kb on - strand at 71.582 kb on - strand at 71.603 kb on + strand at 71.603 kb on + strand at 71.603 kb on + strand at 71.604 kb on - strand at 71.604 kb on - strand at 71.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 69,343 + GFF6163 0.60 +0.3 69,343 + GFF6163 0.60 -1.3 69,343 + GFF6163 0.60 +0.2 69,344 - GFF6163 0.60 +0.3 69,344 - GFF6163 0.60 +0.1 69,344 - GFF6163 0.60 +0.4 69,695 + +1.7 69,695 + -0.1 69,695 + -0.2 69,696 - -1.8 69,696 - -1.0 69,734 + -2.8 69,735 - +1.4 69,735 - -0.8 69,890 + GFF6164 0.19 +0.6 69,890 + GFF6164 0.19 -1.6 69,890 + GFF6164 0.19 -1.6 69,891 - GFF6164 0.19 -1.5 69,891 - GFF6164 0.19 -0.1 70,115 + GFF6164 0.40 -1.1 70,115 + GFF6164 0.40 -2.2 70,116 - GFF6164 0.40 -0.0 70,116 - GFF6164 0.40 +1.7 70,116 - GFF6164 0.40 +0.5 70,116 - GFF6164 0.40 -1.3 70,116 - GFF6164 0.40 +0.7 70,163 + GFF6164 0.45 -1.2 70,163 + GFF6164 0.45 +0.1 70,164 - GFF6164 0.45 +1.1 70,203 - GFF6164 0.49 -0.2 70,203 - GFF6164 0.49 +0.2 70,268 + GFF6164 0.55 -2.3 70,668 - +0.6 70,668 - +0.1 70,796 - -0.0 70,796 - +0.4 70,972 + GFF6165 0.27 -0.4 70,972 + GFF6165 0.27 -0.9 71,146 + GFF6165 0.47 -0.6 71,147 - GFF6165 0.47 -2.1 71,344 + GFF6165 0.70 -0.4 71,344 + GFF6165 0.70 +0.4 71,344 + GFF6165 0.70 -0.3 71,344 + GFF6165 0.70 -0.4 71,344 + GFF6165 0.70 +1.7 71,344 + GFF6165 0.70 -0.1 71,344 + GFF6165 0.70 -0.5 71,345 - GFF6165 0.70 +0.7 71,345 - GFF6165 0.70 -0.6 71,567 - -0.4 71,582 - -0.9 71,603 + -3.0 71,603 + +0.1 71,603 + +0.3 71,604 - -1.1 71,604 - +1.4 71,604 - +0.1
Or see this region's nucleotide sequence