Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6095 and GFF6096 overlap by 4 nucleotides GFF6096 and GFF6097 are separated by 15 nucleotides GFF6097 and GFF6098 are separated by 47 nucleotides
GFF6095 - 1,2-phenylacetyl-CoA epoxidase, subunit C (EC 1.14.13.149), at 156,545 to 157,324
GFF6095
GFF6096 - 1,2-phenylacetyl-CoA epoxidase, subunit D (EC 1.14.13.149), at 157,321 to 157,845
GFF6096
GFF6097 - 1,2-phenylacetyl-CoA epoxidase, subunit E (EC 1.14.13.149), at 157,861 to 158,946
GFF6097
GFF6098 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 158,994 to 160,139
GFF6098
Position (kb)
157
158
159 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 157.070 kb on + strand, within GFF6095 at 157.070 kb on + strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.268 kb on + strand at 157.268 kb on + strand at 157.268 kb on + strand at 157.268 kb on + strand at 157.269 kb on - strand at 157.269 kb on - strand at 157.474 kb on + strand, within GFF6096 at 157.628 kb on - strand, within GFF6096 at 157.795 kb on + strand at 157.795 kb on + strand at 157.843 kb on + strand at 157.843 kb on + strand at 157.844 kb on - strand at 157.844 kb on - strand at 157.984 kb on + strand, within GFF6097 at 158.303 kb on - strand, within GFF6097 at 158.365 kb on + strand, within GFF6097 at 158.503 kb on + strand, within GFF6097 at 158.503 kb on + strand, within GFF6097 at 158.503 kb on + strand, within GFF6097 at 158.503 kb on + strand, within GFF6097 at 158.504 kb on - strand, within GFF6097 at 158.504 kb on - strand, within GFF6097 at 158.504 kb on - strand, within GFF6097 at 158.504 kb on - strand, within GFF6097 at 158.504 kb on - strand, within GFF6097 at 158.885 kb on + strand at 158.886 kb on - strand at 158.886 kb on - strand at 158.886 kb on - strand at 158.947 kb on + strand at 158.948 kb on - strand at 158.951 kb on + strand at 158.951 kb on + strand at 158.952 kb on - strand at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.171 kb on + strand, within GFF6098 at 159.172 kb on - strand, within GFF6098 at 159.172 kb on - strand, within GFF6098 at 159.172 kb on - strand, within GFF6098 at 159.172 kb on - strand, within GFF6098 at 159.489 kb on + strand, within GFF6098 at 159.490 kb on - strand, within GFF6098 at 159.564 kb on + strand, within GFF6098 at 159.565 kb on - strand, within GFF6098 at 159.708 kb on + strand, within GFF6098 at 159.708 kb on + strand, within GFF6098 at 159.708 kb on + strand, within GFF6098 at 159.709 kb on - strand, within GFF6098 at 159.709 kb on - strand, within GFF6098 at 159.709 kb on - strand, within GFF6098 at 159.709 kb on - strand, within GFF6098 at 159.849 kb on + strand, within GFF6098 at 159.849 kb on + strand, within GFF6098 at 159.849 kb on + strand, within GFF6098 at 159.849 kb on + strand, within GFF6098 at 159.850 kb on - strand, within GFF6098 at 159.861 kb on + strand, within GFF6098 at 159.861 kb on + strand, within GFF6098 at 159.861 kb on + strand, within GFF6098
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 157,070 + GFF6095 0.67 -0.6 157,070 + GFF6095 0.67 +1.7 157,071 - GFF6095 0.67 -1.6 157,071 - GFF6095 0.67 -1.3 157,071 - GFF6095 0.67 -1.0 157,071 - GFF6095 0.67 -0.7 157,268 + -0.8 157,268 + +0.7 157,268 + -1.0 157,268 + -1.2 157,269 - +0.7 157,269 - -0.1 157,474 + GFF6096 0.29 +0.0 157,628 - GFF6096 0.58 -0.7 157,795 + +0.1 157,795 + +1.1 157,843 + +0.1 157,843 + +0.1 157,844 - -0.5 157,844 - +0.1 157,984 + GFF6097 0.11 -0.3 158,303 - GFF6097 0.41 -2.2 158,365 + GFF6097 0.46 +1.7 158,503 + GFF6097 0.59 +0.1 158,503 + GFF6097 0.59 +0.5 158,503 + GFF6097 0.59 -1.3 158,503 + GFF6097 0.59 -2.2 158,504 - GFF6097 0.59 +0.2 158,504 - GFF6097 0.59 -1.0 158,504 - GFF6097 0.59 -1.1 158,504 - GFF6097 0.59 -1.6 158,504 - GFF6097 0.59 -0.6 158,885 + +0.4 158,886 - -0.4 158,886 - -0.3 158,886 - -0.4 158,947 + +0.6 158,948 - +0.0 158,951 + +0.5 158,951 + -0.0 158,952 - +0.3 159,171 + GFF6098 0.15 +1.9 159,171 + GFF6098 0.15 +0.4 159,171 + GFF6098 0.15 -1.0 159,171 + GFF6098 0.15 -0.4 159,171 + GFF6098 0.15 -0.2 159,171 + GFF6098 0.15 -0.8 159,171 + GFF6098 0.15 +2.1 159,171 + GFF6098 0.15 -1.1 159,171 + GFF6098 0.15 +1.7 159,171 + GFF6098 0.15 -0.5 159,172 - GFF6098 0.16 -3.1 159,172 - GFF6098 0.16 +0.1 159,172 - GFF6098 0.16 -0.9 159,172 - GFF6098 0.16 -0.8 159,489 + GFF6098 0.43 +0.7 159,490 - GFF6098 0.43 -0.3 159,564 + GFF6098 0.50 -0.3 159,565 - GFF6098 0.50 -0.2 159,708 + GFF6098 0.62 -0.5 159,708 + GFF6098 0.62 +0.4 159,708 + GFF6098 0.62 -0.5 159,709 - GFF6098 0.62 +2.7 159,709 - GFF6098 0.62 -0.6 159,709 - GFF6098 0.62 +0.1 159,709 - GFF6098 0.62 -0.2 159,849 + GFF6098 0.75 -0.2 159,849 + GFF6098 0.75 +0.4 159,849 + GFF6098 0.75 -0.6 159,849 + GFF6098 0.75 +0.5 159,850 - GFF6098 0.75 -0.7 159,861 + GFF6098 0.76 -0.2 159,861 + GFF6098 0.76 +0.3 159,861 + GFF6098 0.76 +0.8
Or see this region's nucleotide sequence