Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5974 and GFF5975 are separated by 10 nucleotides GFF5975 and GFF5976 are separated by 226 nucleotides GFF5976 and GFF5977 overlap by 29 nucleotides GFF5977 and GFF5978 are separated by 56 nucleotides
GFF5974 - hypothetical protein, at 30,031 to 30,378
GFF5974
GFF5975 - Two-component transcriptional response regulator, LuxR family, at 30,389 to 30,964
GFF5975
GFF5976 - Transcriptional regulator, AraC family, at 31,191 to 32,201
GFF5976
GFF5977 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23), at 32,173 to 33,060
GFF5977
GFF5978 - Nucleoside 2-deoxyribosyltransferase, at 33,117 to 33,677
GFF5978
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 30.238 kb on + strand, within GFF5974 at 30.239 kb on - strand, within GFF5974 at 30.239 kb on - strand, within GFF5974 at 30.377 kb on - strand at 30.480 kb on + strand, within GFF5975 at 30.480 kb on + strand, within GFF5975 at 30.480 kb on + strand, within GFF5975 at 30.480 kb on + strand, within GFF5975 at 30.481 kb on - strand, within GFF5975 at 30.481 kb on - strand, within GFF5975 at 30.481 kb on - strand, within GFF5975 at 30.523 kb on - strand, within GFF5975 at 30.523 kb on - strand, within GFF5975 at 30.523 kb on - strand, within GFF5975 at 30.523 kb on - strand, within GFF5975 at 30.755 kb on - strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.855 kb on + strand, within GFF5975 at 30.856 kb on - strand, within GFF5975 at 30.856 kb on - strand, within GFF5975 at 30.856 kb on - strand, within GFF5975 at 31.062 kb on + strand at 31.062 kb on + strand at 31.063 kb on - strand at 31.063 kb on - strand at 31.207 kb on + strand at 31.207 kb on + strand at 31.207 kb on + strand at 31.208 kb on - strand at 31.208 kb on - strand at 31.383 kb on + strand, within GFF5976 at 31.383 kb on + strand, within GFF5976 at 31.902 kb on + strand, within GFF5976 at 31.902 kb on + strand, within GFF5976 at 31.903 kb on - strand, within GFF5976 at 31.903 kb on - strand, within GFF5976 at 32.019 kb on + strand, within GFF5976 at 32.019 kb on + strand, within GFF5976 at 32.019 kb on + strand, within GFF5976 at 32.019 kb on + strand, within GFF5976 at 32.019 kb on + strand, within GFF5976 at 32.019 kb on + strand, within GFF5976 at 32.020 kb on - strand, within GFF5976 at 32.020 kb on - strand, within GFF5976 at 32.020 kb on - strand, within GFF5976 at 32.020 kb on - strand, within GFF5976 at 32.020 kb on - strand, within GFF5976 at 32.020 kb on - strand, within GFF5976 at 32.068 kb on - strand, within GFF5976 at 32.097 kb on + strand, within GFF5976 at 32.098 kb on - strand, within GFF5976 at 32.098 kb on - strand, within GFF5976 at 32.151 kb on + strand at 32.151 kb on + strand at 32.151 kb on + strand at 32.152 kb on - strand at 32.152 kb on - strand at 32.152 kb on - strand at 32.152 kb on - strand at 32.393 kb on + strand, within GFF5977 at 32.393 kb on + strand, within GFF5977 at 32.393 kb on + strand, within GFF5977 at 32.394 kb on - strand, within GFF5977 at 32.394 kb on - strand, within GFF5977 at 32.394 kb on - strand, within GFF5977 at 32.394 kb on - strand, within GFF5977 at 32.394 kb on - strand, within GFF5977 at 32.394 kb on - strand, within GFF5977 at 32.516 kb on + strand, within GFF5977 at 32.516 kb on + strand, within GFF5977 at 32.516 kb on + strand, within GFF5977 at 32.517 kb on - strand, within GFF5977 at 32.517 kb on - strand, within GFF5977 at 32.517 kb on - strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on + strand, within GFF5977 at 32.693 kb on - strand, within GFF5977 at 32.694 kb on - strand, within GFF5977 at 32.694 kb on - strand, within GFF5977 at 32.694 kb on - strand, within GFF5977 at 32.696 kb on + strand, within GFF5977 at 32.696 kb on + strand, within GFF5977 at 32.697 kb on - strand, within GFF5977 at 32.892 kb on - strand, within GFF5977 at 32.892 kb on - strand, within GFF5977 at 33.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 30,238 + GFF5974 0.59 -0.6 30,239 - GFF5974 0.60 -1.9 30,239 - GFF5974 0.60 +0.4 30,377 - -1.0 30,480 + GFF5975 0.16 +0.9 30,480 + GFF5975 0.16 -0.5 30,480 + GFF5975 0.16 +0.1 30,480 + GFF5975 0.16 +0.9 30,481 - GFF5975 0.16 -0.9 30,481 - GFF5975 0.16 -0.2 30,481 - GFF5975 0.16 +0.7 30,523 - GFF5975 0.23 +0.3 30,523 - GFF5975 0.23 +0.5 30,523 - GFF5975 0.23 -0.0 30,523 - GFF5975 0.23 +0.1 30,755 - GFF5975 0.64 -0.2 30,855 + GFF5975 0.81 +0.1 30,855 + GFF5975 0.81 +1.0 30,855 + GFF5975 0.81 -0.9 30,855 + GFF5975 0.81 -0.3 30,855 + GFF5975 0.81 -0.1 30,855 + GFF5975 0.81 +0.5 30,855 + GFF5975 0.81 +0.1 30,855 + GFF5975 0.81 +0.5 30,855 + GFF5975 0.81 -0.1 30,855 + GFF5975 0.81 -0.2 30,855 + GFF5975 0.81 -1.0 30,855 + GFF5975 0.81 -0.3 30,855 + GFF5975 0.81 -2.1 30,855 + GFF5975 0.81 -0.3 30,856 - GFF5975 0.81 -0.8 30,856 - GFF5975 0.81 +1.7 30,856 - GFF5975 0.81 -0.4 31,062 + +0.0 31,062 + -0.2 31,063 - +0.3 31,063 - -0.6 31,207 + -0.3 31,207 + -0.2 31,207 + +0.5 31,208 - +0.3 31,208 - -0.5 31,383 + GFF5976 0.19 -0.1 31,383 + GFF5976 0.19 +0.7 31,902 + GFF5976 0.70 +0.3 31,902 + GFF5976 0.70 -0.0 31,903 - GFF5976 0.70 +0.9 31,903 - GFF5976 0.70 -0.9 32,019 + GFF5976 0.82 +1.9 32,019 + GFF5976 0.82 -1.4 32,019 + GFF5976 0.82 +0.3 32,019 + GFF5976 0.82 +1.0 32,019 + GFF5976 0.82 +1.1 32,019 + GFF5976 0.82 -0.6 32,020 - GFF5976 0.82 +1.3 32,020 - GFF5976 0.82 -0.5 32,020 - GFF5976 0.82 -0.7 32,020 - GFF5976 0.82 -0.0 32,020 - GFF5976 0.82 +0.1 32,020 - GFF5976 0.82 -0.1 32,068 - GFF5976 0.87 +0.1 32,097 + GFF5976 0.90 -0.1 32,098 - GFF5976 0.90 -0.3 32,098 - GFF5976 0.90 +0.3 32,151 + -0.1 32,151 + -0.3 32,151 + -0.2 32,152 - +0.1 32,152 - +0.4 32,152 - +0.9 32,152 - -1.1 32,393 + GFF5977 0.25 -0.0 32,393 + GFF5977 0.25 +0.2 32,393 + GFF5977 0.25 +0.1 32,394 - GFF5977 0.25 -1.0 32,394 - GFF5977 0.25 -0.3 32,394 - GFF5977 0.25 -1.0 32,394 - GFF5977 0.25 -0.1 32,394 - GFF5977 0.25 -0.2 32,394 - GFF5977 0.25 +0.1 32,516 + GFF5977 0.39 -0.2 32,516 + GFF5977 0.39 +0.8 32,516 + GFF5977 0.39 -0.0 32,517 - GFF5977 0.39 +0.3 32,517 - GFF5977 0.39 +0.4 32,517 - GFF5977 0.39 +0.1 32,693 + GFF5977 0.59 -0.2 32,693 + GFF5977 0.59 -1.5 32,693 + GFF5977 0.59 +0.8 32,693 + GFF5977 0.59 +1.3 32,693 + GFF5977 0.59 -0.3 32,693 + GFF5977 0.59 -0.8 32,693 + GFF5977 0.59 +1.5 32,693 - GFF5977 0.59 +0.4 32,694 - GFF5977 0.59 -1.3 32,694 - GFF5977 0.59 -0.2 32,694 - GFF5977 0.59 +0.1 32,696 + GFF5977 0.59 +1.4 32,696 + GFF5977 0.59 -1.4 32,697 - GFF5977 0.59 -0.0 32,892 - GFF5977 0.81 -0.3 32,892 - GFF5977 0.81 -2.0 33,093 - -0.7
Or see this region's nucleotide sequence