Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5708 and GFF5709 are separated by 247 nucleotides GFF5709 and GFF5710 are separated by 172 nucleotides GFF5710 and GFF5711 are separated by 50 nucleotides GFF5711 and GFF5712 overlap by 4 nucleotides GFF5712 and GFF5713 are separated by 91 nucleotides
GFF5708 - Pyridoxal phosphate-dependent aminotransferase, at 81,272 to 82,606
GFF5708
GFF5709 - no description, at 82,854 to 83,099
GFF5709
GFF5710 - no description, at 83,272 to 84,096
GFF5710
GFF5711 - hypothetical protein, at 84,147 to 84,611
GFF5711
GFF5712 - no description, at 84,608 to 84,916
GFF5712
GFF5713 - Flagellar basal-body P-ring formation protein FlgA, at 85,008 to 85,724
GFF5713
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-2
-1
0
1 at 82.636 kb on + strand at 82.637 kb on - strand at 82.653 kb on - strand at 82.681 kb on + strand at 82.681 kb on + strand at 82.682 kb on - strand at 82.838 kb on + strand at 82.838 kb on + strand at 82.838 kb on + strand at 82.838 kb on + strand at 82.838 kb on + strand at 82.839 kb on - strand at 82.839 kb on - strand at 82.839 kb on - strand at 82.839 kb on - strand at 82.839 kb on - strand at 82.906 kb on + strand, within GFF5709 at 82.906 kb on + strand, within GFF5709 at 82.906 kb on + strand, within GFF5709 at 82.907 kb on - strand, within GFF5709 at 82.931 kb on + strand, within GFF5709 at 82.932 kb on - strand, within GFF5709 at 82.932 kb on - strand, within GFF5709 at 82.932 kb on - strand, within GFF5709 at 82.932 kb on - strand, within GFF5709 at 82.932 kb on - strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.935 kb on + strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.936 kb on - strand, within GFF5709 at 82.937 kb on + strand, within GFF5709 at 82.937 kb on + strand, within GFF5709 at 82.938 kb on - strand, within GFF5709 at 83.046 kb on + strand, within GFF5709 at 83.047 kb on - strand, within GFF5709 at 83.047 kb on - strand, within GFF5709 at 83.098 kb on - strand at 83.273 kb on + strand at 83.388 kb on - strand, within GFF5710 at 83.388 kb on - strand, within GFF5710 at 83.399 kb on + strand, within GFF5710 at 83.399 kb on + strand, within GFF5710 at 83.400 kb on - strand, within GFF5710 at 83.651 kb on + strand, within GFF5710 at 83.652 kb on - strand, within GFF5710 at 83.652 kb on - strand, within GFF5710 at 83.652 kb on - strand, within GFF5710 at 83.652 kb on - strand, within GFF5710 at 83.652 kb on - strand, within GFF5710 at 83.652 kb on - strand, within GFF5710 at 84.096 kb on + strand at 84.097 kb on - strand at 84.271 kb on + strand, within GFF5711 at 84.271 kb on + strand, within GFF5711 at 84.271 kb on + strand, within GFF5711 at 84.271 kb on + strand, within GFF5711 at 84.272 kb on - strand, within GFF5711 at 84.272 kb on - strand, within GFF5711 at 84.272 kb on - strand, within GFF5711 at 84.272 kb on - strand, within GFF5711 at 84.404 kb on - strand, within GFF5711 at 84.404 kb on - strand, within GFF5711 at 84.501 kb on - strand, within GFF5711 at 84.504 kb on + strand, within GFF5711 at 84.504 kb on + strand, within GFF5711 at 84.941 kb on + strand at 84.941 kb on + strand at 84.942 kb on - strand at 84.942 kb on - strand at 84.943 kb on + strand at 84.944 kb on - strand at 84.944 kb on - strand at 84.944 kb on - strand at 84.944 kb on - strand at 85.009 kb on + strand at 85.009 kb on + strand at 85.010 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 82,636 + -0.7 82,637 - +0.0 82,653 - -0.2 82,681 + -1.0 82,681 + +0.0 82,682 - -1.8 82,838 + +1.7 82,838 + +0.4 82,838 + -1.0 82,838 + +0.0 82,838 + -1.1 82,839 - -0.9 82,839 - +0.3 82,839 - -0.3 82,839 - -0.1 82,839 - +0.4 82,906 + GFF5709 0.21 -0.6 82,906 + GFF5709 0.21 -0.7 82,906 + GFF5709 0.21 -0.2 82,907 - GFF5709 0.22 +0.2 82,931 + GFF5709 0.31 +0.3 82,932 - GFF5709 0.32 +0.6 82,932 - GFF5709 0.32 -0.7 82,932 - GFF5709 0.32 +0.9 82,932 - GFF5709 0.32 -1.0 82,932 - GFF5709 0.32 +0.0 82,935 + GFF5709 0.33 -0.1 82,935 + GFF5709 0.33 -1.3 82,935 + GFF5709 0.33 -0.7 82,935 + GFF5709 0.33 -0.1 82,935 + GFF5709 0.33 -0.3 82,935 + GFF5709 0.33 +1.1 82,935 + GFF5709 0.33 +0.4 82,935 + GFF5709 0.33 -0.0 82,935 + GFF5709 0.33 -2.5 82,935 + GFF5709 0.33 +0.6 82,935 + GFF5709 0.33 +0.5 82,935 + GFF5709 0.33 +0.2 82,935 + GFF5709 0.33 -0.7 82,935 + GFF5709 0.33 +0.2 82,935 + GFF5709 0.33 +0.3 82,936 - GFF5709 0.33 -0.2 82,936 - GFF5709 0.33 +0.0 82,936 - GFF5709 0.33 -2.1 82,936 - GFF5709 0.33 +0.4 82,936 - GFF5709 0.33 -1.5 82,936 - GFF5709 0.33 -1.7 82,936 - GFF5709 0.33 -1.2 82,936 - GFF5709 0.33 +0.4 82,936 - GFF5709 0.33 -0.1 82,937 + GFF5709 0.34 -0.5 82,937 + GFF5709 0.34 +0.4 82,938 - GFF5709 0.34 -0.1 83,046 + GFF5709 0.78 +0.8 83,047 - GFF5709 0.78 +0.2 83,047 - GFF5709 0.78 -0.6 83,098 - -0.8 83,273 + +0.4 83,388 - GFF5710 0.14 -0.1 83,388 - GFF5710 0.14 +1.4 83,399 + GFF5710 0.15 -0.1 83,399 + GFF5710 0.15 -1.1 83,400 - GFF5710 0.16 +0.9 83,651 + GFF5710 0.46 -0.9 83,652 - GFF5710 0.46 -0.1 83,652 - GFF5710 0.46 -0.1 83,652 - GFF5710 0.46 +0.0 83,652 - GFF5710 0.46 +1.2 83,652 - GFF5710 0.46 -0.3 83,652 - GFF5710 0.46 +0.9 84,096 + -0.8 84,097 - -0.2 84,271 + GFF5711 0.27 -1.0 84,271 + GFF5711 0.27 -1.3 84,271 + GFF5711 0.27 +0.5 84,271 + GFF5711 0.27 -0.2 84,272 - GFF5711 0.27 -0.3 84,272 - GFF5711 0.27 -0.7 84,272 - GFF5711 0.27 -0.1 84,272 - GFF5711 0.27 -0.1 84,404 - GFF5711 0.55 -0.4 84,404 - GFF5711 0.55 -0.3 84,501 - GFF5711 0.76 -0.5 84,504 + GFF5711 0.77 +0.4 84,504 + GFF5711 0.77 -0.3 84,941 + -0.0 84,941 + -1.1 84,942 - -0.1 84,942 - +0.1 84,943 + +0.4 84,944 - -0.2 84,944 - -0.4 84,944 - -2.1 84,944 - +0.4 85,009 + -0.3 85,009 + +0.7 85,010 - -0.6
Or see this region's nucleotide sequence