Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5051 and GFF5052 are separated by 152 nucleotides GFF5052 and GFF5053 overlap by 4 nucleotides GFF5053 and GFF5054 are separated by 59 nucleotides
GFF5051 - MBL-fold metallohydrolase SPO0683, at 151,788 to 152,747
GFF5051
GFF5052 - FAD-dependent oxidoreductase SPO0682, at 152,900 to 154,540
GFF5052
GFF5053 - Uncharacterized protein SPO0681, at 154,537 to 154,749
GFF5053
GFF5054 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5), at 154,809 to 156,119
GFF5054
Position (kb) 152
153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.013 kb on + strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.014 kb on - strand, within GFF5051 at 152.031 kb on + strand, within GFF5051 at 152.031 kb on + strand, within GFF5051 at 152.031 kb on + strand, within GFF5051 at 152.032 kb on - strand, within GFF5051 at 152.032 kb on - strand, within GFF5051 at 152.032 kb on - strand, within GFF5051 at 152.368 kb on - strand, within GFF5051 at 152.382 kb on + strand, within GFF5051 at 152.382 kb on + strand, within GFF5051 at 152.383 kb on - strand, within GFF5051 at 152.383 kb on - strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.526 kb on + strand, within GFF5051 at 152.527 kb on - strand, within GFF5051 at 152.527 kb on - strand, within GFF5051 at 152.527 kb on - strand, within GFF5051 at 152.527 kb on - strand, within GFF5051 at 152.527 kb on - strand, within GFF5051 at 152.527 kb on - strand, within GFF5051 at 152.577 kb on + strand, within GFF5051 at 152.578 kb on - strand, within GFF5051 at 152.578 kb on - strand, within GFF5051 at 152.589 kb on + strand, within GFF5051 at 152.589 kb on + strand, within GFF5051 at 152.589 kb on + strand, within GFF5051 at 152.589 kb on + strand, within GFF5051 at 152.589 kb on + strand, within GFF5051 at 152.589 kb on + strand, within GFF5051 at 152.590 kb on - strand, within GFF5051 at 152.590 kb on - strand, within GFF5051 at 152.590 kb on - strand, within GFF5051 at 152.610 kb on + strand, within GFF5051 at 152.610 kb on + strand, within GFF5051 at 152.610 kb on + strand, within GFF5051 at 152.611 kb on - strand, within GFF5051 at 152.611 kb on - strand, within GFF5051 at 152.611 kb on - strand, within GFF5051 at 152.611 kb on - strand, within GFF5051 at 152.611 kb on - strand, within GFF5051 at 152.665 kb on - strand at 152.753 kb on - strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.897 kb on + strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.898 kb on - strand at 152.909 kb on + strand at 152.909 kb on + strand at 152.909 kb on + strand at 152.910 kb on - strand at 152.910 kb on - strand at 152.930 kb on + strand at 152.930 kb on + strand at 152.930 kb on + strand at 152.930 kb on + strand at 152.931 kb on - strand at 152.931 kb on - strand at 152.931 kb on - strand at 152.931 kb on - strand at 152.931 kb on - strand at 153.212 kb on + strand, within GFF5052 at 153.212 kb on + strand, within GFF5052 at 153.213 kb on - strand, within GFF5052 at 153.213 kb on - strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.275 kb on + strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.276 kb on - strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.278 kb on + strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.279 kb on - strand, within GFF5052 at 153.290 kb on + strand, within GFF5052 at 153.291 kb on - strand, within GFF5052 at 153.291 kb on - strand, within GFF5052 at 153.291 kb on - strand, within GFF5052 at 153.291 kb on - strand, within GFF5052 at 153.291 kb on - strand, within GFF5052 at 153.291 kb on - strand, within GFF5052 at 153.428 kb on + strand, within GFF5052 at 153.770 kb on + strand, within GFF5052 at 153.770 kb on + strand, within GFF5052 at 153.770 kb on + strand, within GFF5052 at 153.770 kb on + strand, within GFF5052 at 153.770 kb on + strand, within GFF5052 at 153.771 kb on - strand, within GFF5052 at 153.771 kb on - strand, within GFF5052 at 153.771 kb on - strand, within GFF5052 at 153.912 kb on + strand, within GFF5052 at 153.912 kb on + strand, within GFF5052 at 153.913 kb on - strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 154.419 kb on - strand at 154.442 kb on + strand at 154.442 kb on + strand at 154.442 kb on + strand at 154.443 kb on - strand at 154.443 kb on - strand at 154.606 kb on + strand, within GFF5053 at 154.606 kb on + strand, within GFF5053 at 154.607 kb on - strand, within GFF5053 at 154.802 kb on - strand at 154.842 kb on + strand at 154.843 kb on - strand at 154.843 kb on - strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.867 kb on - strand at 154.867 kb on - strand at 154.867 kb on - strand at 154.938 kb on + strand at 154.938 kb on + strand at 154.938 kb on + strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 155.004 kb on + strand, within GFF5054 at 155.037 kb on + strand, within GFF5054 at 155.038 kb on - strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.416 kb on - strand, within GFF5054 at 155.430 kb on + strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 152,013 + GFF5051 0.23 -0.4 152,013 + GFF5051 0.23 +0.2 152,013 + GFF5051 0.23 -0.6 152,013 + GFF5051 0.23 +1.3 152,013 + GFF5051 0.23 +1.1 152,013 + GFF5051 0.23 +0.5 152,013 + GFF5051 0.23 +1.8 152,013 + GFF5051 0.23 -0.7 152,013 + GFF5051 0.23 -0.7 152,013 + GFF5051 0.23 -0.3 152,014 - GFF5051 0.24 -0.1 152,014 - GFF5051 0.24 +0.0 152,014 - GFF5051 0.24 +0.5 152,014 - GFF5051 0.24 -1.8 152,014 - GFF5051 0.24 -0.3 152,014 - GFF5051 0.24 +0.9 152,014 - GFF5051 0.24 -0.0 152,014 - GFF5051 0.24 +2.9 152,031 + GFF5051 0.25 -0.2 152,031 + GFF5051 0.25 -0.8 152,031 + GFF5051 0.25 +0.5 152,032 - GFF5051 0.25 -1.9 152,032 - GFF5051 0.25 -0.8 152,032 - GFF5051 0.25 +0.5 152,368 - GFF5051 0.60 +0.1 152,382 + GFF5051 0.62 +0.0 152,382 + GFF5051 0.62 -0.2 152,383 - GFF5051 0.62 +0.2 152,383 - GFF5051 0.62 +0.5 152,526 + GFF5051 0.77 -0.5 152,526 + GFF5051 0.77 +0.3 152,526 + GFF5051 0.77 +0.6 152,526 + GFF5051 0.77 -0.3 152,526 + GFF5051 0.77 -1.3 152,526 + GFF5051 0.77 -0.3 152,526 + GFF5051 0.77 -0.2 152,526 + GFF5051 0.77 +0.3 152,526 + GFF5051 0.77 -1.1 152,526 + GFF5051 0.77 +0.9 152,527 - GFF5051 0.77 +0.3 152,527 - GFF5051 0.77 -1.0 152,527 - GFF5051 0.77 -0.8 152,527 - GFF5051 0.77 -0.1 152,527 - GFF5051 0.77 -0.7 152,527 - GFF5051 0.77 +1.0 152,577 + GFF5051 0.82 -0.4 152,578 - GFF5051 0.82 +1.1 152,578 - GFF5051 0.82 +1.3 152,589 + GFF5051 0.83 +0.3 152,589 + GFF5051 0.83 +0.5 152,589 + GFF5051 0.83 +0.3 152,589 + GFF5051 0.83 +1.3 152,589 + GFF5051 0.83 +0.6 152,589 + GFF5051 0.83 +1.1 152,590 - GFF5051 0.84 -1.1 152,590 - GFF5051 0.84 -0.1 152,590 - GFF5051 0.84 -1.0 152,610 + GFF5051 0.86 -0.2 152,610 + GFF5051 0.86 -1.6 152,610 + GFF5051 0.86 +0.4 152,611 - GFF5051 0.86 +1.9 152,611 - GFF5051 0.86 -0.5 152,611 - GFF5051 0.86 -0.3 152,611 - GFF5051 0.86 +0.0 152,611 - GFF5051 0.86 +0.6 152,665 - -1.0 152,753 - -0.4 152,897 + -1.2 152,897 + -0.1 152,897 + -2.9 152,897 + -1.1 152,897 + +0.9 152,897 + -1.1 152,897 + -1.1 152,897 + -1.8 152,897 + -0.1 152,897 + +0.3 152,897 + -1.0 152,897 + -1.5 152,898 - -0.1 152,898 - -0.6 152,898 - -0.3 152,898 - -0.7 152,898 - +0.0 152,898 - +2.6 152,898 - -0.8 152,898 - -1.2 152,898 - -1.7 152,898 - +0.1 152,909 + -1.1 152,909 + -0.6 152,909 + +0.1 152,910 - +1.9 152,910 - -0.3 152,930 + -1.1 152,930 + -1.3 152,930 + -0.1 152,930 + +1.3 152,931 - -0.6 152,931 - +0.4 152,931 - +1.2 152,931 - -0.7 152,931 - -0.9 153,212 + GFF5052 0.19 -0.1 153,212 + GFF5052 0.19 -0.2 153,213 - GFF5052 0.19 +1.5 153,213 - GFF5052 0.19 -1.8 153,275 + GFF5052 0.23 -0.1 153,275 + GFF5052 0.23 -0.2 153,275 + GFF5052 0.23 +1.4 153,275 + GFF5052 0.23 -0.7 153,275 + GFF5052 0.23 +1.3 153,275 + GFF5052 0.23 -0.1 153,275 + GFF5052 0.23 +0.2 153,275 + GFF5052 0.23 -0.1 153,275 + GFF5052 0.23 -0.1 153,275 + GFF5052 0.23 +0.3 153,276 - GFF5052 0.23 -0.5 153,276 - GFF5052 0.23 +0.5 153,276 - GFF5052 0.23 -0.2 153,276 - GFF5052 0.23 +0.4 153,276 - GFF5052 0.23 +0.5 153,276 - GFF5052 0.23 -1.0 153,276 - GFF5052 0.23 +0.3 153,276 - GFF5052 0.23 -0.5 153,276 - GFF5052 0.23 +1.5 153,276 - GFF5052 0.23 +0.1 153,278 + GFF5052 0.23 +0.6 153,278 + GFF5052 0.23 +0.8 153,278 + GFF5052 0.23 -0.3 153,278 + GFF5052 0.23 -0.6 153,278 + GFF5052 0.23 +3.4 153,278 + GFF5052 0.23 +0.1 153,278 + GFF5052 0.23 -0.7 153,278 + GFF5052 0.23 -0.6 153,278 + GFF5052 0.23 -0.3 153,278 + GFF5052 0.23 -0.3 153,278 + GFF5052 0.23 +0.6 153,279 - GFF5052 0.23 -1.0 153,279 - GFF5052 0.23 -0.1 153,279 - GFF5052 0.23 -0.5 153,279 - GFF5052 0.23 -0.1 153,279 - GFF5052 0.23 +0.7 153,279 - GFF5052 0.23 -0.3 153,279 - GFF5052 0.23 -0.8 153,279 - GFF5052 0.23 +0.9 153,279 - GFF5052 0.23 -0.1 153,279 - GFF5052 0.23 -0.4 153,290 + GFF5052 0.24 +1.3 153,291 - GFF5052 0.24 -0.2 153,291 - GFF5052 0.24 +0.0 153,291 - GFF5052 0.24 -0.2 153,291 - GFF5052 0.24 +0.2 153,291 - GFF5052 0.24 -0.3 153,291 - GFF5052 0.24 +0.4 153,428 + GFF5052 0.32 -0.9 153,770 + GFF5052 0.53 +1.6 153,770 + GFF5052 0.53 -0.7 153,770 + GFF5052 0.53 +1.6 153,770 + GFF5052 0.53 +0.5 153,770 + GFF5052 0.53 -0.1 153,771 - GFF5052 0.53 +0.0 153,771 - GFF5052 0.53 +0.5 153,771 - GFF5052 0.53 -0.2 153,912 + GFF5052 0.62 +0.1 153,912 + GFF5052 0.62 -0.8 153,913 - GFF5052 0.62 +1.9 153,959 + GFF5052 0.65 +0.5 153,959 + GFF5052 0.65 -0.5 153,959 + GFF5052 0.65 +0.7 153,959 + GFF5052 0.65 +1.9 153,959 + GFF5052 0.65 -0.4 153,959 + GFF5052 0.65 +0.6 153,959 + GFF5052 0.65 +0.2 153,959 + GFF5052 0.65 -0.8 153,959 + GFF5052 0.65 +1.0 153,959 + GFF5052 0.65 -0.8 153,959 + GFF5052 0.65 +0.6 153,959 + GFF5052 0.65 -0.1 153,959 + GFF5052 0.65 +0.9 153,959 + GFF5052 0.65 +0.5 153,959 + GFF5052 0.65 +0.0 153,959 + GFF5052 0.65 -0.5 153,959 + GFF5052 0.65 +0.0 153,960 - GFF5052 0.65 -0.2 153,960 - GFF5052 0.65 +0.7 153,960 - GFF5052 0.65 +0.9 153,960 - GFF5052 0.65 -0.1 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 -0.8 153,960 - GFF5052 0.65 +0.1 153,960 - GFF5052 0.65 -0.4 153,960 - GFF5052 0.65 -1.5 153,960 - GFF5052 0.65 -1.3 153,960 - GFF5052 0.65 +0.4 153,960 - GFF5052 0.65 -1.1 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 -1.0 153,960 - GFF5052 0.65 -0.1 153,960 - GFF5052 0.65 -0.4 153,960 - GFF5052 0.65 -0.4 153,960 - GFF5052 0.65 -2.5 154,419 - -1.5 154,442 + -0.4 154,442 + +0.1 154,442 + +0.6 154,443 - +0.0 154,443 - -0.0 154,606 + GFF5053 0.32 -0.5 154,606 + GFF5053 0.32 -0.6 154,607 - GFF5053 0.33 -0.6 154,802 - -0.5 154,842 + -0.6 154,843 - +1.0 154,843 - +0.0 154,866 + -0.5 154,866 + -0.1 154,866 + -0.1 154,866 + +0.1 154,866 + -0.6 154,867 - +0.3 154,867 - +0.5 154,867 - +1.6 154,938 + -0.5 154,938 + -0.6 154,938 + -0.1 154,939 - +0.1 154,939 - -0.5 154,939 - -1.0 154,939 - -0.7 154,939 - +0.4 155,004 + GFF5054 0.15 -0.4 155,037 + GFF5054 0.17 -0.2 155,038 - GFF5054 0.17 +1.9 155,046 + GFF5054 0.18 -0.4 155,046 + GFF5054 0.18 -0.1 155,046 + GFF5054 0.18 -1.7 155,046 + GFF5054 0.18 -1.2 155,047 - GFF5054 0.18 -1.7 155,047 - GFF5054 0.18 +0.7 155,047 - GFF5054 0.18 -0.5 155,047 - GFF5054 0.18 +0.0 155,047 - GFF5054 0.18 -0.7 155,223 + GFF5054 0.32 +0.9 155,223 + GFF5054 0.32 -0.4 155,223 + GFF5054 0.32 -0.1 155,223 + GFF5054 0.32 -0.9 155,223 + GFF5054 0.32 -0.1 155,223 + GFF5054 0.32 -0.3 155,224 - GFF5054 0.32 +0.3 155,224 - GFF5054 0.32 -0.7 155,224 - GFF5054 0.32 -0.8 155,224 - GFF5054 0.32 +0.4 155,224 - GFF5054 0.32 +0.6 155,224 - GFF5054 0.32 +0.7 155,224 - GFF5054 0.32 +0.6 155,415 + GFF5054 0.46 +0.1 155,415 + GFF5054 0.46 +0.2 155,416 - GFF5054 0.46 +0.1 155,430 + GFF5054 0.47 -0.1 155,431 - GFF5054 0.47 -0.1 155,431 - GFF5054 0.47 -1.4 155,431 - GFF5054 0.47 -0.5 155,431 - GFF5054 0.47 +1.1
Or see this region's nucleotide sequence