Strain Fitness in Variovorax sp. SCN45 around GFF501

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF499 and GFF500 are separated by 16 nucleotidesGFF500 and GFF501 are separated by 119 nucleotidesGFF501 and GFF502 are separated by 4 nucleotidesGFF502 and GFF503 are separated by 17 nucleotides GFF499 - hypothetical protein, at 543,375 to 543,683 GFF499 GFF500 - Lysine exporter protein (LYSE/YGGA), at 543,700 to 544,344 GFF500 GFF501 - hypothetical protein, at 544,464 to 544,769 GFF501 GFF502 - Hydrolase, at 544,774 to 545,505 GFF502 GFF503 - Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1), at 545,523 to 546,554 GFF503 Position (kb) 544 545Strain fitness (log2 ratio) -2 -1 0 1 2at 543.481 kb on + strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.570 kb on + strand, within GFF499at 543.570 kb on + strand, within GFF499at 543.570 kb on + strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.836 kb on + strand, within GFF500at 543.836 kb on + strand, within GFF500at 543.836 kb on + strand, within GFF500at 543.836 kb on + strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.100 kb on + strand, within GFF500at 544.101 kb on - strand, within GFF500at 544.101 kb on - strand, within GFF500at 544.101 kb on - strand, within GFF500at 544.397 kb on + strandat 544.397 kb on + strandat 544.397 kb on + strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.399 kb on + strandat 544.400 kb on - strandat 544.437 kb on - strandat 544.477 kb on - strandat 544.477 kb on - strandat 544.477 kb on - strandat 544.477 kb on - strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.500 kb on + strand, within GFF501at 544.500 kb on + strand, within GFF501at 544.501 kb on - strand, within GFF501at 544.501 kb on - strand, within GFF501at 544.578 kb on + strand, within GFF501at 544.578 kb on + strand, within GFF501at 544.578 kb on + strand, within GFF501at 544.579 kb on - strand, within GFF501at 544.704 kb on + strand, within GFF501at 544.734 kb on + strand, within GFF501at 544.734 kb on + strand, within GFF501at 544.735 kb on - strand, within GFF501at 544.735 kb on - strand, within GFF501at 544.747 kb on - strandat 544.767 kb on + strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 544.768 kb on - strandat 545.141 kb on + strand, within GFF502at 545.141 kb on + strand, within GFF502at 545.517 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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543,481 + GFF499 0.34 -0.6
543,482 - GFF499 0.35 +1.0
543,482 - GFF499 0.35 -0.2
543,482 - GFF499 0.35 -0.9
543,482 - GFF499 0.35 +0.2
543,570 + GFF499 0.63 +1.4
543,570 + GFF499 0.63 -0.3
543,570 + GFF499 0.63 +2.0
543,571 - GFF499 0.63 -0.4
543,571 - GFF499 0.63 +1.4
543,571 - GFF499 0.63 -0.7
543,571 - GFF499 0.63 +1.0
543,836 + GFF500 0.21 -0.7
543,836 + GFF500 0.21 -0.1
543,836 + GFF500 0.21 +0.5
543,836 + GFF500 0.21 +0.8
543,837 - GFF500 0.21 +0.2
543,837 - GFF500 0.21 -0.4
543,837 - GFF500 0.21 -0.2
543,837 - GFF500 0.21 -0.6
543,837 - GFF500 0.21 -0.8
544,091 + GFF500 0.61 +0.2
544,091 + GFF500 0.61 -0.8
544,091 + GFF500 0.61 -0.3
544,091 + GFF500 0.61 +1.8
544,092 - GFF500 0.61 +1.5
544,092 - GFF500 0.61 +1.2
544,092 - GFF500 0.61 +0.8
544,092 - GFF500 0.61 -0.6
544,092 - GFF500 0.61 +0.1
544,100 + GFF500 0.62 +0.1
544,101 - GFF500 0.62 -0.8
544,101 - GFF500 0.62 +0.4
544,101 - GFF500 0.62 +0.4
544,397 + +0.9
544,397 + -0.5
544,397 + -0.7
544,398 - -0.1
544,398 - +0.8
544,398 - -0.1
544,398 - -0.4
544,398 - -0.6
544,398 - -0.6
544,398 - -0.6
544,399 + +0.0
544,400 - +0.6
544,437 - -0.5
544,477 - -1.3
544,477 - -0.8
544,477 - +0.3
544,477 - -0.7
544,485 + +1.0
544,485 + +0.7
544,485 + -0.8
544,485 + -0.8
544,485 + -0.5
544,485 + -0.1
544,486 - +0.5
544,486 - -0.1
544,486 - -1.2
544,486 - -1.7
544,486 - -0.3
544,486 - +0.4
544,500 + GFF501 0.12 +0.4
544,500 + GFF501 0.12 -0.6
544,501 - GFF501 0.12 -0.2
544,501 - GFF501 0.12 +0.5
544,578 + GFF501 0.37 -1.8
544,578 + GFF501 0.37 +1.4
544,578 + GFF501 0.37 -0.4
544,579 - GFF501 0.38 -1.3
544,704 + GFF501 0.78 -0.8
544,734 + GFF501 0.88 +0.4
544,734 + GFF501 0.88 -0.2
544,735 - GFF501 0.89 +0.2
544,735 - GFF501 0.89 -1.6
544,747 - -0.7
544,767 + -0.6
544,768 - -0.2
544,768 - +0.3
544,768 - -1.3
544,768 - -0.3
544,768 - +0.1
544,768 - +0.1
544,768 - +0.6
544,768 - -0.9
544,768 - -0.6
545,141 + GFF502 0.50 -0.2
545,141 + GFF502 0.50 -0.3
545,517 + -0.2

Or see this region's nucleotide sequence