Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4731 and GFF4732 are separated by 48 nucleotides GFF4732 and GFF4733 are separated by 9 nucleotides GFF4733 and GFF4734 overlap by 4 nucleotides
GFF4731 - Assimilatory nitrate reductase large subunit (EC 1.7.99.4), at 94,717 to 97,509
GFF4731
GFF4732 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 97,558 to 97,953
GFF4732
GFF4733 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 97,963 to 98,754
GFF4733
GFF4734 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 98,751 to 101,219
GFF4734
Position (kb)
97
98
99 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 97.029 kb on - strand, within GFF4731 at 97.067 kb on + strand, within GFF4731 at 97.068 kb on - strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.190 kb on + strand, within GFF4731 at 97.191 kb on - strand, within GFF4731 at 97.481 kb on + strand at 97.560 kb on - strand at 97.964 kb on + strand at 97.964 kb on + strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.982 kb on + strand at 97.983 kb on - strand at 98.258 kb on + strand, within GFF4733 at 98.259 kb on - strand, within GFF4733 at 98.345 kb on + strand, within GFF4733 at 98.480 kb on + strand, within GFF4733 at 98.481 kb on - strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.921 kb on - strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.945 kb on - strand at 98.945 kb on - strand at 98.965 kb on + strand at 98.966 kb on - strand at 99.007 kb on + strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.085 kb on - strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.197 kb on - strand, within GFF4734 at 99.311 kb on - strand, within GFF4734 at 99.424 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.599 kb on - strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.682 kb on + strand, within GFF4734 at 99.682 kb on + strand, within GFF4734
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 97,029 - GFF4731 0.83 -0.4 97,067 + GFF4731 0.84 +0.3 97,068 - GFF4731 0.84 -0.2 97,157 + GFF4731 0.87 -0.5 97,157 + GFF4731 0.87 -2.0 97,157 + GFF4731 0.87 +0.7 97,158 - GFF4731 0.87 -0.0 97,158 - GFF4731 0.87 -0.0 97,158 - GFF4731 0.87 -2.4 97,160 + GFF4731 0.87 -0.8 97,160 + GFF4731 0.87 -0.1 97,160 + GFF4731 0.87 +0.4 97,160 + GFF4731 0.87 -0.1 97,160 + GFF4731 0.87 -0.4 97,160 + GFF4731 0.87 +0.4 97,161 - GFF4731 0.88 -1.4 97,161 - GFF4731 0.88 +1.3 97,161 - GFF4731 0.88 -0.2 97,161 - GFF4731 0.88 +0.7 97,161 - GFF4731 0.88 -0.7 97,161 - GFF4731 0.88 +1.6 97,190 + GFF4731 0.89 -1.0 97,191 - GFF4731 0.89 -1.2 97,481 + -0.2 97,560 - -0.4 97,964 + -1.1 97,964 + -1.4 97,965 - -0.5 97,965 - +0.5 97,965 - +1.2 97,965 - -0.7 97,982 + +0.4 97,983 - -0.1 98,258 + GFF4733 0.37 -0.6 98,259 - GFF4733 0.37 -0.5 98,345 + GFF4733 0.48 -0.4 98,480 + GFF4733 0.65 -1.7 98,481 - GFF4733 0.65 -0.8 98,609 + GFF4733 0.82 -0.1 98,609 + GFF4733 0.82 +0.3 98,610 - GFF4733 0.82 -1.7 98,610 - GFF4733 0.82 +0.9 98,921 - -0.5 98,944 + -0.4 98,944 + +0.0 98,944 + -2.2 98,945 - +0.9 98,945 - +2.9 98,965 + +0.0 98,966 - -0.5 99,007 + GFF4734 0.10 +0.9 99,008 - GFF4734 0.10 -0.4 99,008 - GFF4734 0.10 -0.0 99,084 + GFF4734 0.13 +0.8 99,084 + GFF4734 0.13 -0.2 99,085 - GFF4734 0.14 -0.1 99,088 + GFF4734 0.14 -1.4 99,088 + GFF4734 0.14 -0.6 99,088 + GFF4734 0.14 +0.5 99,088 + GFF4734 0.14 -1.2 99,088 + GFF4734 0.14 +1.0 99,088 + GFF4734 0.14 -0.4 99,089 - GFF4734 0.14 -0.4 99,089 - GFF4734 0.14 -0.3 99,196 + GFF4734 0.18 -0.4 99,196 + GFF4734 0.18 -0.5 99,197 - GFF4734 0.18 +0.6 99,311 - GFF4734 0.23 +0.0 99,424 + GFF4734 0.27 +0.9 99,502 + GFF4734 0.30 -0.2 99,502 + GFF4734 0.30 -1.1 99,502 + GFF4734 0.30 -0.6 99,502 + GFF4734 0.30 -0.6 99,503 - GFF4734 0.30 -0.1 99,503 - GFF4734 0.30 +0.8 99,598 + GFF4734 0.34 -1.1 99,598 + GFF4734 0.34 +0.2 99,599 - GFF4734 0.34 +0.2 99,601 + GFF4734 0.34 -0.1 99,601 + GFF4734 0.34 -1.2 99,601 + GFF4734 0.34 +0.5 99,601 + GFF4734 0.34 -0.9 99,601 + GFF4734 0.34 -0.7 99,601 + GFF4734 0.34 -1.5 99,601 + GFF4734 0.34 -0.3 99,602 - GFF4734 0.34 -0.1 99,602 - GFF4734 0.34 -0.2 99,682 + GFF4734 0.38 +0.7 99,682 + GFF4734 0.38 +1.0
Or see this region's nucleotide sequence