Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4606 and GFF4607 overlap by 4 nucleotides GFF4607 and GFF4608 are separated by 62 nucleotides
GFF4606 - 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16), at 247,365 to 248,561
GFF4606
GFF4607 - Putative DNA methylase, at 248,558 to 248,974
GFF4607
GFF4608 - 3-hydroxyacyl-CoA dehydrogenase [fadN-fadA-fadE operon] (EC 1.1.1.35) / Enoyl-CoA hydratase [fadN-fadA-fadE operon] (EC 4.2.1.17), at 249,037 to 251,436
GFF4608
Position (kb) 248
249 Strain fitness (log2 ratio) -2
-1
0
1
2 at 247.640 kb on - strand, within GFF4606 at 248.479 kb on + strand at 248.539 kb on + strand at 248.539 kb on + strand at 248.539 kb on + strand at 248.539 kb on + strand at 248.539 kb on + strand at 248.539 kb on + strand at 248.540 kb on - strand at 248.540 kb on - strand at 248.540 kb on - strand at 248.540 kb on - strand at 248.540 kb on - strand at 248.540 kb on - strand at 248.705 kb on + strand, within GFF4607 at 248.751 kb on + strand, within GFF4607 at 248.752 kb on - strand, within GFF4607 at 248.829 kb on + strand, within GFF4607 at 248.829 kb on + strand, within GFF4607 at 248.829 kb on + strand, within GFF4607 at 248.829 kb on + strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.830 kb on - strand, within GFF4607 at 248.974 kb on + strand at 248.975 kb on - strand at 249.143 kb on + strand at 249.143 kb on + strand at 249.143 kb on + strand at 249.144 kb on - strand at 249.371 kb on + strand, within GFF4608 at 249.371 kb on + strand, within GFF4608 at 249.371 kb on + strand, within GFF4608 at 249.371 kb on + strand, within GFF4608 at 249.372 kb on - strand, within GFF4608 at 249.372 kb on - strand, within GFF4608 at 249.372 kb on - strand, within GFF4608 at 249.372 kb on - strand, within GFF4608 at 249.419 kb on + strand, within GFF4608 at 249.420 kb on - strand, within GFF4608 at 249.420 kb on - strand, within GFF4608 at 249.554 kb on + strand, within GFF4608 at 249.554 kb on + strand, within GFF4608 at 249.554 kb on + strand, within GFF4608 at 249.554 kb on + strand, within GFF4608 at 249.554 kb on + strand, within GFF4608 at 249.555 kb on - strand, within GFF4608 at 249.555 kb on - strand, within GFF4608 at 249.555 kb on - strand, within GFF4608 at 249.611 kb on + strand, within GFF4608 at 249.612 kb on - strand, within GFF4608 at 249.612 kb on - strand, within GFF4608 at 249.612 kb on - strand, within GFF4608 at 249.612 kb on - strand, within GFF4608 at 249.644 kb on + strand, within GFF4608 at 249.644 kb on + strand, within GFF4608 at 249.860 kb on + strand, within GFF4608 at 249.860 kb on + strand, within GFF4608 at 249.861 kb on - strand, within GFF4608 at 249.861 kb on - strand, within GFF4608 at 249.861 kb on - strand, within GFF4608 at 249.861 kb on - strand, within GFF4608
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 247,640 - GFF4606 0.23 -1.3 248,479 + -0.5 248,539 + -0.1 248,539 + -0.5 248,539 + -2.0 248,539 + -1.0 248,539 + +0.5 248,539 + +0.1 248,540 - -0.7 248,540 - -0.8 248,540 - -1.7 248,540 - -0.5 248,540 - -1.1 248,540 - -0.7 248,705 + GFF4607 0.35 +0.2 248,751 + GFF4607 0.46 +0.8 248,752 - GFF4607 0.47 -1.8 248,829 + GFF4607 0.65 -2.4 248,829 + GFF4607 0.65 -1.5 248,829 + GFF4607 0.65 +0.4 248,829 + GFF4607 0.65 -0.2 248,830 - GFF4607 0.65 +2.4 248,830 - GFF4607 0.65 -0.0 248,830 - GFF4607 0.65 +0.7 248,830 - GFF4607 0.65 +1.0 248,830 - GFF4607 0.65 +0.4 248,830 - GFF4607 0.65 -0.4 248,830 - GFF4607 0.65 +0.2 248,974 + -0.2 248,975 - -0.7 249,143 + +0.6 249,143 + -0.0 249,143 + +0.2 249,144 - -0.2 249,371 + GFF4608 0.14 -0.2 249,371 + GFF4608 0.14 -0.1 249,371 + GFF4608 0.14 -0.3 249,371 + GFF4608 0.14 -0.3 249,372 - GFF4608 0.14 -0.8 249,372 - GFF4608 0.14 +0.9 249,372 - GFF4608 0.14 +0.2 249,372 - GFF4608 0.14 -0.8 249,419 + GFF4608 0.16 -0.4 249,420 - GFF4608 0.16 +0.7 249,420 - GFF4608 0.16 -0.4 249,554 + GFF4608 0.22 -0.1 249,554 + GFF4608 0.22 -0.9 249,554 + GFF4608 0.22 -0.2 249,554 + GFF4608 0.22 +0.3 249,554 + GFF4608 0.22 -0.5 249,555 - GFF4608 0.22 +0.3 249,555 - GFF4608 0.22 -2.1 249,555 - GFF4608 0.22 +0.2 249,611 + GFF4608 0.24 -0.0 249,612 - GFF4608 0.24 -0.9 249,612 - GFF4608 0.24 +0.1 249,612 - GFF4608 0.24 -1.3 249,612 - GFF4608 0.24 -1.0 249,644 + GFF4608 0.25 -1.0 249,644 + GFF4608 0.25 +1.0 249,860 + GFF4608 0.34 -0.4 249,860 + GFF4608 0.34 +0.5 249,861 - GFF4608 0.34 -0.3 249,861 - GFF4608 0.34 +0.5 249,861 - GFF4608 0.34 -0.2 249,861 - GFF4608 0.34 -0.2
Or see this region's nucleotide sequence