Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4314 and GFF4315 overlap by 4 nucleotides GFF4315 and GFF4316 overlap by 4 nucleotides GFF4316 and GFF4317 are separated by 4 nucleotides GFF4317 and GFF4318 are separated by 21 nucleotides
GFF4314 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 234,859 to 235,662
GFF4314
GFF4315 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 235,659 to 236,528
GFF4315
GFF4316 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 236,525 to 238,327
GFF4316
GFF4317 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 238,332 to 239,210
GFF4317
GFF4318 - no description, at 239,232 to 240,428
GFF4318
Position (kb) 236
237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 235.601 kb on + strand at 235.754 kb on - strand, within GFF4315 at 235.831 kb on + strand, within GFF4315 at 236.417 kb on - strand, within GFF4315 at 236.489 kb on + strand at 236.490 kb on - strand at 236.490 kb on - strand at 236.559 kb on + strand at 236.560 kb on - strand at 236.560 kb on - strand at 236.560 kb on - strand at 236.681 kb on + strand at 236.682 kb on - strand at 237.284 kb on + strand, within GFF4316 at 237.615 kb on + strand, within GFF4316 at 237.616 kb on - strand, within GFF4316 at 237.810 kb on + strand, within GFF4316 at 237.810 kb on + strand, within GFF4316 at 237.810 kb on + strand, within GFF4316 at 237.811 kb on - strand, within GFF4316 at 238.080 kb on + strand, within GFF4316 at 238.080 kb on + strand, within GFF4316 at 238.081 kb on - strand, within GFF4316 at 238.333 kb on + strand at 238.786 kb on + strand, within GFF4317 at 239.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 235,601 + -0.8 235,754 - GFF4315 0.11 -0.4 235,831 + GFF4315 0.20 -0.6 236,417 - GFF4315 0.87 -0.7 236,489 + +0.3 236,490 - -0.4 236,490 - +0.7 236,559 + +3.4 236,560 - -0.9 236,560 - -0.7 236,560 - -0.2 236,681 + -2.9 236,682 - -0.4 237,284 + GFF4316 0.42 +0.8 237,615 + GFF4316 0.60 +0.2 237,616 - GFF4316 0.61 -0.5 237,810 + GFF4316 0.71 -1.2 237,810 + GFF4316 0.71 +0.5 237,810 + GFF4316 0.71 +1.5 237,811 - GFF4316 0.71 -0.6 238,080 + GFF4316 0.86 +1.0 238,080 + GFF4316 0.86 +0.1 238,081 - GFF4316 0.86 +0.5 238,333 + +0.5 238,786 + GFF4317 0.52 -0.3 239,164 + -0.4
Or see this region's nucleotide sequence