Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4260 and GFF4261 are separated by 160 nucleotides GFF4261 and GFF4262 overlap by 4 nucleotides GFF4262 and GFF4263 are separated by 126 nucleotides
GFF4260 - hypothetical protein, at 175,017 to 179,039
GFF4260
GFF4261 - RNA polymerase ECF-type sigma factor, at 179,200 to 179,751
GFF4261
GFF4262 - hypothetical protein, at 179,748 to 180,893
GFF4262
GFF4263 - TonB-dependent siderophore receptor, at 181,020 to 183,482
GFF4263
Position (kb) 179
180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 179.112 kb on + strand at 179.112 kb on + strand at 179.195 kb on - strand at 179.212 kb on + strand at 179.212 kb on + strand at 179.213 kb on - strand at 179.213 kb on - strand at 179.213 kb on - strand at 179.215 kb on + strand at 179.216 kb on - strand at 179.290 kb on + strand, within GFF4261 at 179.290 kb on + strand, within GFF4261 at 179.354 kb on - strand, within GFF4261 at 179.354 kb on - strand, within GFF4261 at 179.354 kb on - strand, within GFF4261 at 179.509 kb on + strand, within GFF4261 at 179.509 kb on + strand, within GFF4261 at 179.509 kb on + strand, within GFF4261 at 179.509 kb on + strand, within GFF4261 at 179.509 kb on + strand, within GFF4261 at 179.510 kb on - strand, within GFF4261 at 179.510 kb on - strand, within GFF4261 at 179.510 kb on - strand, within GFF4261 at 179.735 kb on + strand at 179.735 kb on + strand at 179.736 kb on - strand at 179.736 kb on - strand at 179.736 kb on - strand at 179.736 kb on - strand at 179.817 kb on + strand at 179.817 kb on + strand at 179.818 kb on - strand at 179.949 kb on + strand, within GFF4262 at 179.950 kb on - strand, within GFF4262 at 179.950 kb on - strand, within GFF4262 at 180.246 kb on + strand, within GFF4262 at 180.247 kb on - strand, within GFF4262 at 180.247 kb on - strand, within GFF4262 at 180.247 kb on - strand, within GFF4262 at 180.247 kb on - strand, within GFF4262 at 180.339 kb on + strand, within GFF4262 at 180.340 kb on - strand, within GFF4262 at 180.980 kb on - strand at 180.980 kb on - strand at 180.997 kb on + strand at 180.998 kb on - strand at 181.458 kb on + strand, within GFF4263 at 181.459 kb on - strand, within GFF4263 at 181.693 kb on - strand, within GFF4263 at 181.693 kb on - strand, within GFF4263 at 181.737 kb on + strand, within GFF4263 at 181.737 kb on + strand, within GFF4263 at 181.737 kb on + strand, within GFF4263 at 181.738 kb on - strand, within GFF4263 at 181.738 kb on - strand, within GFF4263 at 181.738 kb on - strand, within GFF4263 at 181.882 kb on - strand, within GFF4263
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 179,112 + -0.2 179,112 + +0.8 179,195 - -0.6 179,212 + -2.5 179,212 + +0.0 179,213 - +0.0 179,213 - -0.6 179,213 - +0.0 179,215 + +0.0 179,216 - -0.4 179,290 + GFF4261 0.16 +1.5 179,290 + GFF4261 0.16 -0.2 179,354 - GFF4261 0.28 -0.1 179,354 - GFF4261 0.28 -1.0 179,354 - GFF4261 0.28 -0.0 179,509 + GFF4261 0.56 -0.1 179,509 + GFF4261 0.56 -1.0 179,509 + GFF4261 0.56 -0.1 179,509 + GFF4261 0.56 -1.2 179,509 + GFF4261 0.56 +1.4 179,510 - GFF4261 0.56 +0.6 179,510 - GFF4261 0.56 -0.3 179,510 - GFF4261 0.56 -2.9 179,735 + -0.7 179,735 + -0.0 179,736 - -0.8 179,736 - +3.2 179,736 - +0.1 179,736 - -1.0 179,817 + +0.6 179,817 + +0.6 179,818 - -0.7 179,949 + GFF4262 0.18 -0.6 179,950 - GFF4262 0.18 -0.6 179,950 - GFF4262 0.18 -0.8 180,246 + GFF4262 0.43 -0.9 180,247 - GFF4262 0.44 +0.5 180,247 - GFF4262 0.44 -0.2 180,247 - GFF4262 0.44 -1.2 180,247 - GFF4262 0.44 -0.8 180,339 + GFF4262 0.52 +0.0 180,340 - GFF4262 0.52 -0.3 180,980 - -0.3 180,980 - +1.0 180,997 + +0.1 180,998 - +1.0 181,458 + GFF4263 0.18 -0.4 181,459 - GFF4263 0.18 +0.6 181,693 - GFF4263 0.27 +1.4 181,693 - GFF4263 0.27 +0.5 181,737 + GFF4263 0.29 +0.6 181,737 + GFF4263 0.29 -1.4 181,737 + GFF4263 0.29 +0.5 181,738 - GFF4263 0.29 +1.4 181,738 - GFF4263 0.29 -0.4 181,738 - GFF4263 0.29 +0.1 181,882 - GFF4263 0.35 -0.6
Or see this region's nucleotide sequence