Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4083 and GFF4084 are separated by 44 nucleotides GFF4084 and GFF4085 are separated by 10 nucleotides GFF4085 and GFF4086 are separated by 96 nucleotides GFF4086 and GFF4087 are separated by 33 nucleotides
GFF4083 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 295,468 to 297,903
GFF4083
GFF4084 - hypothetical protein, at 297,948 to 298,166
GFF4084
GFF4085 - Transcriptional regulator, LysR family, at 298,177 to 299,115
GFF4085
GFF4086 - no description, at 299,212 to 299,664
GFF4086
GFF4087 - Cystathionine beta-lyase (EC 4.4.1.8), at 299,698 to 300,867
GFF4087
Position (kb) 298
299
300 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 297.719 kb on - strand at 297.719 kb on - strand at 297.991 kb on - strand, within GFF4084 at 297.991 kb on - strand, within GFF4084 at 297.991 kb on - strand, within GFF4084 at 298.102 kb on - strand, within GFF4084 at 298.297 kb on + strand, within GFF4085 at 298.437 kb on - strand, within GFF4085 at 299.116 kb on - strand at 299.116 kb on - strand at 299.117 kb on + strand at 299.118 kb on - strand at 299.118 kb on - strand at 299.118 kb on - strand at 299.118 kb on - strand at 299.161 kb on - strand at 299.174 kb on + strand at 299.175 kb on - strand at 299.175 kb on - strand at 299.587 kb on + strand, within GFF4086 at 299.588 kb on - strand, within GFF4086 at 299.846 kb on - strand, within GFF4087 at 300.011 kb on - strand, within GFF4087
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 297,719 - -0.0 297,719 - -2.9 297,991 - GFF4084 0.20 -1.8 297,991 - GFF4084 0.20 -0.7 297,991 - GFF4084 0.20 +0.0 298,102 - GFF4084 0.70 +1.3 298,297 + GFF4085 0.13 -1.3 298,437 - GFF4085 0.28 -1.0 299,116 - -2.1 299,116 - -1.9 299,117 + -1.3 299,118 - -0.4 299,118 - +2.4 299,118 - -1.4 299,118 - -0.3 299,161 - -1.5 299,174 + -0.3 299,175 - -0.5 299,175 - +1.0 299,587 + GFF4086 0.83 +0.2 299,588 - GFF4086 0.83 +0.5 299,846 - GFF4087 0.13 +0.1 300,011 - GFF4087 0.27 +0.3
Or see this region's nucleotide sequence