Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3689 and GFF3690 overlap by 4 nucleotides GFF3690 and GFF3691 overlap by 41 nucleotides
GFF3689 - FIG01198523: hypothetical protein, at 187,944 to 189,146
GFF3689
GFF3690 - ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose) / ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose), at 189,143 to 190,690
GFF3690
GFF3691 - ABC transporter, permease protein (cluster 2, ribose/xylose/arabinose/galactose), at 190,650 to 191,684
GFF3691
Position (kb)
189
190
191 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 188.311 kb on + strand, within GFF3689 at 188.312 kb on - strand, within GFF3689 at 188.312 kb on - strand, within GFF3689 at 188.312 kb on - strand, within GFF3689 at 188.464 kb on - strand, within GFF3689 at 188.464 kb on - strand, within GFF3689 at 188.479 kb on - strand, within GFF3689 at 188.479 kb on - strand, within GFF3689 at 188.622 kb on + strand, within GFF3689 at 188.622 kb on + strand, within GFF3689 at 188.623 kb on - strand, within GFF3689 at 189.012 kb on + strand, within GFF3689 at 189.012 kb on + strand, within GFF3689 at 189.012 kb on + strand, within GFF3689 at 189.013 kb on - strand, within GFF3689 at 189.013 kb on - strand, within GFF3689 at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.058 kb on - strand at 189.058 kb on - strand at 189.269 kb on + strand at 189.269 kb on + strand at 189.269 kb on + strand at 189.269 kb on + strand at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 190.174 kb on + strand, within GFF3690 at 190.174 kb on + strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.178 kb on + strand, within GFF3690 at 190.179 kb on - strand, within GFF3690 at 190.179 kb on - strand, within GFF3690 at 190.179 kb on - strand, within GFF3690 at 190.262 kb on + strand, within GFF3690 at 190.263 kb on - strand, within GFF3690 at 190.326 kb on - strand, within GFF3690 at 190.326 kb on - strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.485 kb on - strand, within GFF3690 at 191.146 kb on - strand, within GFF3691 at 191.146 kb on - strand, within GFF3691 at 191.146 kb on - strand, within GFF3691 at 191.196 kb on + strand, within GFF3691 at 191.196 kb on + strand, within GFF3691 at 191.196 kb on + strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.208 kb on + strand, within GFF3691 at 191.208 kb on + strand, within GFF3691 at 191.209 kb on - strand, within GFF3691 at 191.209 kb on - strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.212 kb on - strand, within GFF3691 at 191.212 kb on - strand, within GFF3691 at 191.281 kb on + strand, within GFF3691 at 191.281 kb on + strand, within GFF3691 at 191.282 kb on - strand, within GFF3691 at 191.282 kb on - strand, within GFF3691 at 191.299 kb on - strand, within GFF3691 at 191.307 kb on + strand, within GFF3691 at 191.308 kb on - strand, within GFF3691 at 191.347 kb on - strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.386 kb on - strand, within GFF3691 at 191.386 kb on - strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.428 kb on - strand, within GFF3691 at 191.428 kb on - strand, within GFF3691 at 191.428 kb on - strand, within GFF3691 at 191.616 kb on + strand at 191.616 kb on + strand at 191.616 kb on + strand at 191.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 188,311 + GFF3689 0.31 -0.9 188,312 - GFF3689 0.31 +1.3 188,312 - GFF3689 0.31 -0.1 188,312 - GFF3689 0.31 +0.8 188,464 - GFF3689 0.43 -1.0 188,464 - GFF3689 0.43 -0.3 188,479 - GFF3689 0.44 -0.8 188,479 - GFF3689 0.44 +0.4 188,622 + GFF3689 0.56 +1.7 188,622 + GFF3689 0.56 -0.4 188,623 - GFF3689 0.56 +1.0 189,012 + GFF3689 0.89 -0.5 189,012 + GFF3689 0.89 -0.2 189,012 + GFF3689 0.89 -0.7 189,013 - GFF3689 0.89 -0.7 189,013 - GFF3689 0.89 +0.5 189,057 + -0.2 189,057 + +0.7 189,057 + -0.1 189,057 + +1.0 189,057 + -1.3 189,057 + -0.4 189,058 - -0.3 189,058 - +0.6 189,269 + -0.2 189,269 + +0.2 189,269 + +0.7 189,269 + +0.1 189,755 + GFF3690 0.40 -0.7 189,755 + GFF3690 0.40 -1.1 189,755 + GFF3690 0.40 -1.1 189,755 + GFF3690 0.40 -0.2 189,755 + GFF3690 0.40 -0.8 189,755 + GFF3690 0.40 +0.4 189,755 + GFF3690 0.40 -0.7 189,756 - GFF3690 0.40 +0.1 189,756 - GFF3690 0.40 -1.5 189,756 - GFF3690 0.40 -0.8 189,756 - GFF3690 0.40 +0.4 189,756 - GFF3690 0.40 -1.0 189,756 - GFF3690 0.40 -0.7 190,174 + GFF3690 0.67 +0.0 190,174 + GFF3690 0.67 -2.5 190,175 - GFF3690 0.67 -0.1 190,175 - GFF3690 0.67 +0.5 190,175 - GFF3690 0.67 +0.0 190,175 - GFF3690 0.67 -0.5 190,175 - GFF3690 0.67 -3.5 190,178 + GFF3690 0.67 -1.2 190,179 - GFF3690 0.67 -1.7 190,179 - GFF3690 0.67 +0.8 190,179 - GFF3690 0.67 +1.5 190,262 + GFF3690 0.72 -0.5 190,263 - GFF3690 0.72 -1.7 190,326 - GFF3690 0.76 -1.2 190,326 - GFF3690 0.76 -0.5 190,484 + GFF3690 0.87 +0.3 190,484 + GFF3690 0.87 -0.5 190,484 + GFF3690 0.87 -0.9 190,484 + GFF3690 0.87 -0.3 190,484 + GFF3690 0.87 -0.7 190,484 + GFF3690 0.87 +0.6 190,485 - GFF3690 0.87 -0.5 191,146 - GFF3691 0.48 -0.2 191,146 - GFF3691 0.48 -1.4 191,146 - GFF3691 0.48 -0.2 191,196 + GFF3691 0.53 -1.1 191,196 + GFF3691 0.53 -0.3 191,196 + GFF3691 0.53 -0.7 191,197 - GFF3691 0.53 -0.8 191,197 - GFF3691 0.53 -0.2 191,197 - GFF3691 0.53 -1.0 191,197 - GFF3691 0.53 -0.5 191,208 + GFF3691 0.54 -1.3 191,208 + GFF3691 0.54 +0.1 191,209 - GFF3691 0.54 +2.2 191,209 - GFF3691 0.54 -0.1 191,211 + GFF3691 0.54 +0.5 191,211 + GFF3691 0.54 -1.0 191,211 + GFF3691 0.54 -0.0 191,211 + GFF3691 0.54 -0.6 191,212 - GFF3691 0.54 +0.4 191,212 - GFF3691 0.54 +0.3 191,281 + GFF3691 0.61 -0.7 191,281 + GFF3691 0.61 +1.0 191,282 - GFF3691 0.61 -0.6 191,282 - GFF3691 0.61 -0.3 191,299 - GFF3691 0.63 -1.7 191,307 + GFF3691 0.63 -0.3 191,308 - GFF3691 0.64 +0.9 191,347 - GFF3691 0.67 -1.2 191,385 + GFF3691 0.71 +0.1 191,385 + GFF3691 0.71 -0.4 191,385 + GFF3691 0.71 +0.3 191,385 + GFF3691 0.71 -1.0 191,385 + GFF3691 0.71 +0.3 191,385 + GFF3691 0.71 -0.2 191,386 - GFF3691 0.71 -0.6 191,386 - GFF3691 0.71 +0.3 191,427 + GFF3691 0.75 -1.6 191,427 + GFF3691 0.75 -2.2 191,427 + GFF3691 0.75 -0.4 191,427 + GFF3691 0.75 -1.0 191,428 - GFF3691 0.75 -0.1 191,428 - GFF3691 0.75 +0.7 191,428 - GFF3691 0.75 -0.5 191,616 + -0.4 191,616 + +3.4 191,616 + -0.4 191,617 - -1.2
Or see this region's nucleotide sequence