Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3416 and GFF3417 overlap by 4 nucleotides GFF3417 and GFF3418 overlap by 8 nucleotides GFF3418 and GFF3419 are separated by 74 nucleotides
GFF3416 - Alkanesulfonates transport system permease protein, at 240,183 to 240,962
GFF3416
GFF3417 - Alkanesulfonates transport system permease protein, at 240,959 to 241,729
GFF3417
GFF3418 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 241,722 to 242,501
GFF3418
GFF3419 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 242,576 to 243,535
GFF3419
Position (kb) 241
242
243 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 240.836 kb on + strand, within GFF3416 at 240.946 kb on + strand at 241.653 kb on + strand at 241.654 kb on - strand at 241.670 kb on + strand at 241.775 kb on - strand at 241.900 kb on + strand, within GFF3418 at 241.900 kb on + strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.940 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.905 kb on - strand, within GFF3419 at 243.042 kb on + strand, within GFF3419 at 243.117 kb on + strand, within GFF3419 at 243.369 kb on + strand, within GFF3419 at 243.369 kb on + strand, within GFF3419 at 243.370 kb on - strand, within GFF3419 at 243.370 kb on - strand, within GFF3419
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 240,836 + GFF3416 0.84 +2.9 240,946 + +1.3 241,653 + -0.2 241,654 - +0.1 241,670 + -0.9 241,775 - +0.6 241,900 + GFF3418 0.23 +0.7 241,900 + GFF3418 0.23 -0.4 241,901 - GFF3418 0.23 +0.9 241,901 - GFF3418 0.23 -1.6 241,940 + GFF3418 0.28 +1.3 242,334 + GFF3418 0.78 -1.4 242,334 + GFF3418 0.78 -0.8 242,730 + GFF3419 0.16 +0.6 242,730 + GFF3419 0.16 +0.9 242,730 + GFF3419 0.16 -0.4 242,730 + GFF3419 0.16 +3.2 242,730 + GFF3419 0.16 -0.4 242,731 - GFF3419 0.16 -0.9 242,731 - GFF3419 0.16 -0.0 242,731 - GFF3419 0.16 -2.0 242,905 - GFF3419 0.34 -0.4 243,042 + GFF3419 0.49 -0.3 243,117 + GFF3419 0.56 +1.3 243,369 + GFF3419 0.83 -1.1 243,369 + GFF3419 0.83 -0.1 243,370 - GFF3419 0.83 -0.2 243,370 - GFF3419 0.83 +0.9
Or see this region's nucleotide sequence