Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3260 and GFF3261 are separated by 91 nucleotides GFF3261 and GFF3262 are separated by 33 nucleotides GFF3262 and GFF3263 are separated by 112 nucleotides
GFF3260 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 80,117 to 81,628
GFF3260
GFF3261 - FIG003003: hypothetical protein, at 81,720 to 82,499
GFF3261
GFF3262 - Polymer-forming bactofilin, at 82,533 to 83,033
GFF3262
GFF3263 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 83,146 to 84,066
GFF3263
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.997 kb on + strand, within GFF3260 at 80.997 kb on + strand, within GFF3260 at 80.998 kb on - strand, within GFF3260 at 80.998 kb on - strand, within GFF3260 at 80.998 kb on - strand, within GFF3260 at 81.069 kb on + strand, within GFF3260 at 81.069 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.085 kb on - strand, within GFF3260 at 81.085 kb on - strand, within GFF3260 at 81.085 kb on - strand, within GFF3260 at 81.180 kb on + strand, within GFF3260 at 81.180 kb on + strand, within GFF3260 at 81.180 kb on + strand, within GFF3260 at 81.180 kb on + strand, within GFF3260 at 81.181 kb on - strand, within GFF3260 at 81.181 kb on - strand, within GFF3260 at 81.181 kb on - strand, within GFF3260 at 81.190 kb on - strand, within GFF3260 at 81.216 kb on + strand, within GFF3260 at 81.216 kb on + strand, within GFF3260 at 81.217 kb on - strand, within GFF3260 at 81.217 kb on - strand, within GFF3260 at 81.234 kb on + strand, within GFF3260 at 81.234 kb on + strand, within GFF3260 at 81.235 kb on - strand, within GFF3260 at 81.235 kb on - strand, within GFF3260 at 81.235 kb on - strand, within GFF3260 at 81.235 kb on - strand, within GFF3260 at 81.262 kb on + strand, within GFF3260 at 81.262 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.303 kb on + strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.304 kb on - strand, within GFF3260 at 81.516 kb on + strand at 81.516 kb on + strand at 81.516 kb on + strand at 81.516 kb on + strand at 81.516 kb on + strand at 81.516 kb on + strand at 81.516 kb on + strand at 81.517 kb on - strand at 81.517 kb on - strand at 81.517 kb on - strand at 81.517 kb on - strand at 81.689 kb on - strand at 81.755 kb on + strand at 81.755 kb on + strand at 81.756 kb on - strand at 81.756 kb on - strand at 81.756 kb on - strand at 81.771 kb on + strand at 81.771 kb on + strand at 81.771 kb on + strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.772 kb on - strand at 81.795 kb on + strand at 81.795 kb on + strand at 81.796 kb on - strand at 81.796 kb on - strand at 81.831 kb on + strand, within GFF3261 at 81.831 kb on + strand, within GFF3261 at 81.831 kb on + strand, within GFF3261 at 81.831 kb on + strand, within GFF3261 at 81.832 kb on - strand, within GFF3261 at 81.832 kb on - strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.873 kb on + strand, within GFF3261 at 81.874 kb on - strand, within GFF3261 at 81.874 kb on - strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.876 kb on + strand, within GFF3261 at 81.877 kb on - strand, within GFF3261 at 81.877 kb on - strand, within GFF3261 at 81.877 kb on - strand, within GFF3261 at 81.877 kb on - strand, within GFF3261 at 82.197 kb on + strand, within GFF3261 at 82.198 kb on - strand, within GFF3261 at 82.198 kb on - strand, within GFF3261 at 82.198 kb on - strand, within GFF3261 at 82.198 kb on - strand, within GFF3261 at 82.198 kb on - strand, within GFF3261 at 82.198 kb on - strand, within GFF3261 at 82.332 kb on + strand, within GFF3261 at 82.333 kb on - strand, within GFF3261 at 82.333 kb on - strand, within GFF3261 at 82.413 kb on + strand, within GFF3261 at 82.413 kb on + strand, within GFF3261 at 82.413 kb on + strand, within GFF3261 at 82.601 kb on - strand, within GFF3262 at 82.698 kb on + strand, within GFF3262 at 82.698 kb on + strand, within GFF3262 at 82.699 kb on - strand, within GFF3262 at 82.699 kb on - strand, within GFF3262 at 82.699 kb on - strand, within GFF3262 at 82.699 kb on - strand, within GFF3262 at 82.699 kb on - strand, within GFF3262 at 83.027 kb on + strand at 83.027 kb on + strand at 83.028 kb on - strand at 83.028 kb on - strand at 83.060 kb on - strand at 83.060 kb on - strand at 83.064 kb on + strand at 83.064 kb on + strand at 83.064 kb on + strand at 83.064 kb on + strand at 83.065 kb on - strand at 83.065 kb on - strand at 83.065 kb on - strand at 83.065 kb on - strand at 83.117 kb on + strand at 83.118 kb on - strand at 83.118 kb on - strand at 83.416 kb on + strand, within GFF3263 at 83.416 kb on + strand, within GFF3263 at 83.416 kb on + strand, within GFF3263 at 83.416 kb on + strand, within GFF3263 at 83.416 kb on + strand, within GFF3263 at 83.416 kb on + strand, within GFF3263 at 83.417 kb on - strand, within GFF3263 at 83.417 kb on - strand, within GFF3263 at 83.417 kb on - strand, within GFF3263 at 83.417 kb on - strand, within GFF3263 at 83.417 kb on - strand, within GFF3263 at 83.417 kb on - strand, within GFF3263 at 83.455 kb on + strand, within GFF3263 at 83.455 kb on + strand, within GFF3263 at 83.470 kb on + strand, within GFF3263 at 83.471 kb on - strand, within GFF3263 at 83.471 kb on - strand, within GFF3263
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 80,739 + GFF3260 0.41 -0.2 80,739 + GFF3260 0.41 +2.5 80,739 + GFF3260 0.41 +1.6 80,739 + GFF3260 0.41 -0.1 80,739 + GFF3260 0.41 -1.3 80,739 + GFF3260 0.41 -0.9 80,739 + GFF3260 0.41 -0.6 80,740 - GFF3260 0.41 -0.7 80,740 - GFF3260 0.41 -1.4 80,740 - GFF3260 0.41 +0.0 80,740 - GFF3260 0.41 -0.6 80,740 - GFF3260 0.41 -0.4 80,997 + GFF3260 0.58 +0.1 80,997 + GFF3260 0.58 -0.7 80,998 - GFF3260 0.58 +0.1 80,998 - GFF3260 0.58 -0.5 80,998 - GFF3260 0.58 +1.2 81,069 + GFF3260 0.63 -0.3 81,069 + GFF3260 0.63 +0.7 81,084 + GFF3260 0.64 -1.4 81,084 + GFF3260 0.64 +1.3 81,084 + GFF3260 0.64 +0.9 81,084 + GFF3260 0.64 +0.1 81,085 - GFF3260 0.64 +1.6 81,085 - GFF3260 0.64 +0.8 81,085 - GFF3260 0.64 -1.1 81,180 + GFF3260 0.70 -0.5 81,180 + GFF3260 0.70 +0.2 81,180 + GFF3260 0.70 +0.3 81,180 + GFF3260 0.70 -1.0 81,181 - GFF3260 0.70 -0.1 81,181 - GFF3260 0.70 +0.1 81,181 - GFF3260 0.70 +0.7 81,190 - GFF3260 0.71 -1.8 81,216 + GFF3260 0.73 -1.3 81,216 + GFF3260 0.73 -1.6 81,217 - GFF3260 0.73 +0.2 81,217 - GFF3260 0.73 -0.4 81,234 + GFF3260 0.74 -1.3 81,234 + GFF3260 0.74 +1.0 81,235 - GFF3260 0.74 -0.5 81,235 - GFF3260 0.74 -0.5 81,235 - GFF3260 0.74 -0.1 81,235 - GFF3260 0.74 -0.1 81,262 + GFF3260 0.76 -0.9 81,262 + GFF3260 0.76 -0.4 81,303 + GFF3260 0.78 -0.3 81,303 + GFF3260 0.78 -0.2 81,303 + GFF3260 0.78 -0.8 81,303 + GFF3260 0.78 +0.2 81,303 + GFF3260 0.78 +0.2 81,303 + GFF3260 0.78 -1.8 81,303 + GFF3260 0.78 -1.4 81,303 + GFF3260 0.78 +1.0 81,303 + GFF3260 0.78 +1.5 81,303 + GFF3260 0.78 +0.1 81,304 - GFF3260 0.79 +0.7 81,304 - GFF3260 0.79 +0.4 81,304 - GFF3260 0.79 +0.7 81,304 - GFF3260 0.79 -0.7 81,304 - GFF3260 0.79 +0.0 81,304 - GFF3260 0.79 -1.7 81,304 - GFF3260 0.79 +0.2 81,516 + +0.2 81,516 + -0.1 81,516 + -2.3 81,516 + -1.8 81,516 + -0.1 81,516 + +0.9 81,516 + -0.6 81,517 - +0.1 81,517 - -2.3 81,517 - -0.6 81,517 - -2.1 81,689 - -0.8 81,755 + +2.2 81,755 + -0.8 81,756 - -0.5 81,756 - +0.2 81,756 - -0.8 81,771 + +0.1 81,771 + -1.8 81,771 + -0.3 81,772 - -0.2 81,772 - -0.6 81,772 - -1.4 81,772 - -0.8 81,772 - -0.8 81,772 - -0.1 81,772 - -1.8 81,772 - -1.0 81,772 - -0.1 81,772 - -0.8 81,772 - -0.2 81,795 + -0.6 81,795 + +0.4 81,796 - +0.9 81,796 - +0.0 81,831 + GFF3261 0.14 +1.9 81,831 + GFF3261 0.14 -1.3 81,831 + GFF3261 0.14 -0.1 81,831 + GFF3261 0.14 +0.2 81,832 - GFF3261 0.14 -1.3 81,832 - GFF3261 0.14 +0.3 81,873 + GFF3261 0.20 -0.2 81,873 + GFF3261 0.20 +1.9 81,873 + GFF3261 0.20 +2.1 81,873 + GFF3261 0.20 +0.7 81,873 + GFF3261 0.20 -0.9 81,873 + GFF3261 0.20 -0.6 81,873 + GFF3261 0.20 +1.4 81,874 - GFF3261 0.20 -0.7 81,874 - GFF3261 0.20 +0.8 81,876 + GFF3261 0.20 +1.3 81,876 + GFF3261 0.20 -0.8 81,876 + GFF3261 0.20 +0.0 81,876 + GFF3261 0.20 -0.1 81,876 + GFF3261 0.20 +0.1 81,876 + GFF3261 0.20 -0.3 81,876 + GFF3261 0.20 +0.9 81,877 - GFF3261 0.20 +0.7 81,877 - GFF3261 0.20 +0.3 81,877 - GFF3261 0.20 +0.9 81,877 - GFF3261 0.20 -0.2 82,197 + GFF3261 0.61 +1.2 82,198 - GFF3261 0.61 -0.5 82,198 - GFF3261 0.61 +0.7 82,198 - GFF3261 0.61 +0.5 82,198 - GFF3261 0.61 -1.2 82,198 - GFF3261 0.61 +0.0 82,198 - GFF3261 0.61 +0.4 82,332 + GFF3261 0.78 +0.6 82,333 - GFF3261 0.79 +2.2 82,333 - GFF3261 0.79 -0.5 82,413 + GFF3261 0.89 -0.7 82,413 + GFF3261 0.89 -0.1 82,413 + GFF3261 0.89 -0.1 82,601 - GFF3262 0.14 +1.1 82,698 + GFF3262 0.33 -0.4 82,698 + GFF3262 0.33 -0.8 82,699 - GFF3262 0.33 +0.3 82,699 - GFF3262 0.33 -0.3 82,699 - GFF3262 0.33 -0.1 82,699 - GFF3262 0.33 -0.7 82,699 - GFF3262 0.33 +1.5 83,027 + +0.7 83,027 + -0.6 83,028 - +0.6 83,028 - -0.1 83,060 - -0.3 83,060 - -0.8 83,064 + +1.7 83,064 + -0.6 83,064 + -0.3 83,064 + -1.6 83,065 - +0.3 83,065 - +0.2 83,065 - -0.4 83,065 - -0.9 83,117 + +0.2 83,118 - -1.4 83,118 - +1.0 83,416 + GFF3263 0.29 -1.5 83,416 + GFF3263 0.29 +0.5 83,416 + GFF3263 0.29 -0.3 83,416 + GFF3263 0.29 -0.1 83,416 + GFF3263 0.29 +0.3 83,416 + GFF3263 0.29 -0.8 83,417 - GFF3263 0.29 +0.3 83,417 - GFF3263 0.29 -0.3 83,417 - GFF3263 0.29 -0.3 83,417 - GFF3263 0.29 -0.3 83,417 - GFF3263 0.29 -1.8 83,417 - GFF3263 0.29 -0.6 83,455 + GFF3263 0.34 -1.4 83,455 + GFF3263 0.34 -0.2 83,470 + GFF3263 0.35 +0.2 83,471 - GFF3263 0.35 -0.6 83,471 - GFF3263 0.35 -0.7
Or see this region's nucleotide sequence