Strain Fitness in Variovorax sp. SCN45 around GFF3221

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3219 and GFF3220 are separated by 24 nucleotidesGFF3220 and GFF3221 are separated by 3 nucleotidesGFF3221 and GFF3222 are separated by 128 nucleotidesGFF3222 and GFF3223 are separated by 1 nucleotides GFF3219 - hypothetical protein, at 38,717 to 39,154 GFF3219 GFF3220 - no description, at 39,179 to 39,790 GFF3220 GFF3221 - hypothetical protein, at 39,794 to 43,843 GFF3221 GFF3222 - no description, at 43,972 to 44,388 GFF3222 GFF3223 - no description, at 44,390 to 45,007 GFF3223 Position (kb) 39 40 41 42 43 44Strain fitness (log2 ratio) -2 -1 0 1 2at 38.974 kb on - strand, within GFF3219at 38.974 kb on - strand, within GFF3219at 38.984 kb on + strand, within GFF3219at 38.985 kb on - strand, within GFF3219at 38.985 kb on - strand, within GFF3219at 38.985 kb on - strand, within GFF3219at 38.985 kb on - strand, within GFF3219at 38.985 kb on - strand, within GFF3219at 39.045 kb on + strand, within GFF3219at 39.045 kb on + strand, within GFF3219at 39.045 kb on + strand, within GFF3219at 39.045 kb on + strand, within GFF3219at 39.045 kb on + strand, within GFF3219at 39.046 kb on - strand, within GFF3219at 39.147 kb on + strandat 39.180 kb on + strandat 39.181 kb on - strandat 39.636 kb on + strand, within GFF3220at 39.771 kb on + strandat 39.816 kb on + strandat 40.117 kb on + strandat 40.117 kb on + strandat 40.377 kb on + strand, within GFF3221at 40.457 kb on - strand, within GFF3221at 40.457 kb on - strand, within GFF3221at 40.541 kb on + strand, within GFF3221at 40.828 kb on + strand, within GFF3221at 40.829 kb on - strand, within GFF3221at 40.829 kb on - strand, within GFF3221at 41.535 kb on + strand, within GFF3221at 41.583 kb on + strand, within GFF3221at 41.583 kb on + strand, within GFF3221at 41.583 kb on + strand, within GFF3221at 41.584 kb on - strand, within GFF3221at 41.685 kb on + strand, within GFF3221at 41.685 kb on + strand, within GFF3221at 41.686 kb on - strand, within GFF3221at 41.828 kb on - strand, within GFF3221at 42.093 kb on + strand, within GFF3221at 42.105 kb on + strand, within GFF3221at 42.105 kb on + strand, within GFF3221at 42.106 kb on - strand, within GFF3221at 42.135 kb on + strand, within GFF3221at 42.444 kb on + strand, within GFF3221at 42.445 kb on - strand, within GFF3221at 42.445 kb on - strand, within GFF3221at 42.445 kb on - strand, within GFF3221at 42.498 kb on + strand, within GFF3221at 42.498 kb on + strand, within GFF3221at 42.498 kb on + strand, within GFF3221at 42.498 kb on + strand, within GFF3221at 42.498 kb on + strand, within GFF3221at 42.498 kb on + strand, within GFF3221at 42.540 kb on + strand, within GFF3221at 42.793 kb on - strand, within GFF3221at 42.793 kb on - strand, within GFF3221at 42.793 kb on - strand, within GFF3221at 43.023 kb on + strand, within GFF3221at 43.024 kb on - strand, within GFF3221at 43.024 kb on - strand, within GFF3221at 43.024 kb on - strand, within GFF3221at 43.024 kb on - strand, within GFF3221at 43.044 kb on + strand, within GFF3221at 43.044 kb on + strand, within GFF3221at 43.044 kb on + strand, within GFF3221at 43.045 kb on - strand, within GFF3221at 43.080 kb on + strand, within GFF3221at 43.081 kb on - strand, within GFF3221at 43.081 kb on - strand, within GFF3221at 43.153 kb on - strand, within GFF3221at 43.206 kb on + strand, within GFF3221at 43.320 kb on + strand, within GFF3221at 43.320 kb on + strand, within GFF3221at 43.320 kb on + strand, within GFF3221at 43.320 kb on + strand, within GFF3221at 43.320 kb on + strand, within GFF3221at 43.320 kb on + strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.321 kb on - strand, within GFF3221at 43.327 kb on - strand, within GFF3221at 43.398 kb on + strand, within GFF3221at 43.398 kb on + strand, within GFF3221at 43.398 kb on + strand, within GFF3221at 43.399 kb on - strand, within GFF3221at 43.399 kb on - strand, within GFF3221at 43.399 kb on - strand, within GFF3221at 43.399 kb on - strand, within GFF3221at 43.512 kb on + strandat 43.513 kb on - strandat 43.621 kb on - strandat 43.677 kb on + strandat 43.677 kb on + strandat 43.677 kb on + strandat 43.678 kb on - strandat 43.678 kb on - strandat 43.678 kb on - strandat 43.678 kb on - strandat 43.831 kb on + strandat 44.001 kb on + strandat 44.072 kb on + strand, within GFF3222

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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38,974 - GFF3219 0.59 -2.6
38,974 - GFF3219 0.59 -0.3
38,984 + GFF3219 0.61 +1.9
38,985 - GFF3219 0.61 -0.5
38,985 - GFF3219 0.61 +1.1
38,985 - GFF3219 0.61 -0.1
38,985 - GFF3219 0.61 -1.1
38,985 - GFF3219 0.61 +1.1
39,045 + GFF3219 0.75 -0.7
39,045 + GFF3219 0.75 +0.3
39,045 + GFF3219 0.75 -0.3
39,045 + GFF3219 0.75 +1.2
39,045 + GFF3219 0.75 +0.3
39,046 - GFF3219 0.75 +0.4
39,147 + -2.4
39,180 + -0.8
39,181 - +0.2
39,636 + GFF3220 0.75 -0.3
39,771 + -1.1
39,816 + +0.4
40,117 + +0.9
40,117 + +0.3
40,377 + GFF3221 0.14 -1.3
40,457 - GFF3221 0.16 -0.9
40,457 - GFF3221 0.16 +0.0
40,541 + GFF3221 0.18 -2.4
40,828 + GFF3221 0.26 -1.7
40,829 - GFF3221 0.26 -1.7
40,829 - GFF3221 0.26 -0.7
41,535 + GFF3221 0.43 +0.5
41,583 + GFF3221 0.44 -0.4
41,583 + GFF3221 0.44 +0.6
41,583 + GFF3221 0.44 +0.5
41,584 - GFF3221 0.44 -0.9
41,685 + GFF3221 0.47 -0.8
41,685 + GFF3221 0.47 -2.1
41,686 - GFF3221 0.47 -0.9
41,828 - GFF3221 0.50 -1.4
42,093 + GFF3221 0.57 +0.7
42,105 + GFF3221 0.57 -0.4
42,105 + GFF3221 0.57 -1.6
42,106 - GFF3221 0.57 +0.5
42,135 + GFF3221 0.58 -0.1
42,444 + GFF3221 0.65 -0.3
42,445 - GFF3221 0.65 +0.2
42,445 - GFF3221 0.65 -0.7
42,445 - GFF3221 0.65 -1.0
42,498 + GFF3221 0.67 -0.4
42,498 + GFF3221 0.67 -1.4
42,498 + GFF3221 0.67 +1.2
42,498 + GFF3221 0.67 -1.5
42,498 + GFF3221 0.67 -0.2
42,498 + GFF3221 0.67 -0.4
42,540 + GFF3221 0.68 +0.5
42,793 - GFF3221 0.74 -1.1
42,793 - GFF3221 0.74 -0.3
42,793 - GFF3221 0.74 +0.0
43,023 + GFF3221 0.80 -0.2
43,024 - GFF3221 0.80 +1.1
43,024 - GFF3221 0.80 -0.1
43,024 - GFF3221 0.80 -0.5
43,024 - GFF3221 0.80 -1.8
43,044 + GFF3221 0.80 +0.3
43,044 + GFF3221 0.80 -0.5
43,044 + GFF3221 0.80 +0.5
43,045 - GFF3221 0.80 -0.1
43,080 + GFF3221 0.81 -0.4
43,081 - GFF3221 0.81 -0.6
43,081 - GFF3221 0.81 +0.9
43,153 - GFF3221 0.83 -1.0
43,206 + GFF3221 0.84 -1.3
43,320 + GFF3221 0.87 -0.6
43,320 + GFF3221 0.87 +0.3
43,320 + GFF3221 0.87 -0.9
43,320 + GFF3221 0.87 +1.0
43,320 + GFF3221 0.87 -0.4
43,320 + GFF3221 0.87 -1.5
43,321 - GFF3221 0.87 -0.5
43,321 - GFF3221 0.87 +0.3
43,321 - GFF3221 0.87 -0.4
43,321 - GFF3221 0.87 +0.7
43,321 - GFF3221 0.87 +0.1
43,321 - GFF3221 0.87 +0.1
43,321 - GFF3221 0.87 -0.4
43,327 - GFF3221 0.87 -0.1
43,398 + GFF3221 0.89 -0.7
43,398 + GFF3221 0.89 -1.1
43,398 + GFF3221 0.89 +1.5
43,399 - GFF3221 0.89 +0.5
43,399 - GFF3221 0.89 -1.1
43,399 - GFF3221 0.89 -0.4
43,399 - GFF3221 0.89 -0.2
43,512 + -0.6
43,513 - -0.4
43,621 - -0.2
43,677 + -0.7
43,677 + +0.5
43,677 + -0.4
43,678 - -0.5
43,678 - +0.5
43,678 - -2.0
43,678 - -2.2
43,831 + -1.3
44,001 + -0.6
44,072 + GFF3222 0.24 -1.3

Or see this region's nucleotide sequence