Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3044 and GFF3045 are separated by 884 nucleotides GFF3045 and GFF3046 are separated by 64 nucleotides GFF3046 and GFF3047 overlap by 4 nucleotides
GFF3044 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 477,677 to 480,034
GFF3044
GFF3045 - Glutamine ABC transporter, substrate-binding protein GlnH, at 480,919 to 481,761
GFF3045
GFF3046 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 481,826 to 482,617
GFF3046
GFF3047 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 482,614 to 483,393
GFF3047
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 479.998 kb on + strand at 479.999 kb on - strand at 479.999 kb on - strand at 479.999 kb on - strand at 480.020 kb on - strand at 480.303 kb on - strand at 480.303 kb on - strand at 480.399 kb on - strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.417 kb on + strand at 480.686 kb on + strand at 480.687 kb on - strand at 480.717 kb on + strand at 480.918 kb on - strand at 480.983 kb on + strand at 481.049 kb on + strand, within GFF3045 at 481.049 kb on + strand, within GFF3045 at 481.403 kb on + strand, within GFF3045 at 481.403 kb on + strand, within GFF3045 at 481.404 kb on - strand, within GFF3045 at 481.406 kb on + strand, within GFF3045 at 481.406 kb on + strand, within GFF3045 at 481.407 kb on - strand, within GFF3045
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 479,998 + -0.3 479,999 - +0.4 479,999 - -0.2 479,999 - -0.5 480,020 - -1.2 480,303 - -0.7 480,303 - +2.8 480,399 - -0.4 480,401 + -0.9 480,401 + +0.3 480,401 + +0.3 480,401 + -1.1 480,401 + -1.2 480,417 + -0.7 480,686 + -0.5 480,687 - -1.3 480,717 + -0.8 480,918 - -0.7 480,983 + -0.1 481,049 + GFF3045 0.15 -0.5 481,049 + GFF3045 0.15 -1.5 481,403 + GFF3045 0.57 +0.0 481,403 + GFF3045 0.57 -2.8 481,404 - GFF3045 0.58 -0.4 481,406 + GFF3045 0.58 -0.8 481,406 + GFF3045 0.58 +0.1 481,407 - GFF3045 0.58 -0.7
Or see this region's nucleotide sequence