Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3029 and GFF3030 overlap by 1 nucleotides GFF3030 and GFF3031 are separated by 74 nucleotides
GFF3029 - T6SS component TssM (IcmF/VasK), at 457,341 to 461,426
GFF3029
GFF3030 - Uncharacterized protein ImpA, at 461,426 to 462,625
GFF3030
GFF3031 - hypothetical protein, at 462,700 to 464,118
GFF3031
Position (kb)
461
462
463 Strain fitness (log2 ratio)
-2
-1
0
1 at 460.578 kb on + strand, within GFF3029 at 460.578 kb on + strand, within GFF3029 at 460.579 kb on - strand, within GFF3029 at 460.579 kb on - strand, within GFF3029 at 460.602 kb on + strand, within GFF3029 at 460.603 kb on - strand, within GFF3029 at 460.603 kb on - strand, within GFF3029 at 460.603 kb on - strand, within GFF3029 at 460.615 kb on - strand, within GFF3029 at 461.053 kb on - strand at 461.564 kb on + strand, within GFF3030 at 462.314 kb on + strand, within GFF3030 at 462.315 kb on - strand, within GFF3030 at 462.315 kb on - strand, within GFF3030 at 462.315 kb on - strand, within GFF3030 at 462.315 kb on - strand, within GFF3030 at 462.318 kb on + strand, within GFF3030 at 462.318 kb on + strand, within GFF3030 at 462.319 kb on - strand, within GFF3030 at 462.319 kb on - strand, within GFF3030 at 462.437 kb on + strand, within GFF3030 at 462.438 kb on - strand, within GFF3030 at 462.438 kb on - strand, within GFF3030 at 462.438 kb on - strand, within GFF3030 at 462.438 kb on - strand, within GFF3030 at 462.464 kb on + strand, within GFF3030 at 462.501 kb on - strand, within GFF3030 at 462.694 kb on + strand at 462.694 kb on + strand at 462.699 kb on + strand at 462.699 kb on + strand at 462.699 kb on + strand at 462.699 kb on + strand at 462.716 kb on + strand at 462.716 kb on + strand at 462.716 kb on + strand at 462.716 kb on + strand at 462.716 kb on + strand at 462.717 kb on - strand at 462.717 kb on - strand at 462.717 kb on - strand at 462.717 kb on - strand at 462.717 kb on - strand at 462.717 kb on - strand at 462.731 kb on + strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.748 kb on + strand at 462.748 kb on + strand at 462.748 kb on + strand at 462.748 kb on + strand at 462.748 kb on + strand at 462.749 kb on - strand at 462.749 kb on - strand at 462.749 kb on - strand at 462.749 kb on - strand at 462.820 kb on + strand at 462.821 kb on - strand at 462.821 kb on - strand at 462.821 kb on - strand at 462.821 kb on - strand at 462.822 kb on - strand at 462.838 kb on - strand at 462.865 kb on + strand, within GFF3031 at 462.997 kb on - strand, within GFF3031 at 463.040 kb on + strand, within GFF3031 at 463.040 kb on + strand, within GFF3031 at 463.041 kb on - strand, within GFF3031 at 463.041 kb on - strand, within GFF3031 at 463.231 kb on + strand, within GFF3031 at 463.232 kb on - strand, within GFF3031 at 463.385 kb on + strand, within GFF3031 at 463.385 kb on + strand, within GFF3031 at 463.385 kb on + strand, within GFF3031 at 463.385 kb on + strand, within GFF3031 at 463.386 kb on - strand, within GFF3031 at 463.386 kb on - strand, within GFF3031 at 463.386 kb on - strand, within GFF3031 at 463.386 kb on - strand, within GFF3031 at 463.386 kb on - strand, within GFF3031 at 463.389 kb on + strand, within GFF3031 at 463.389 kb on + strand, within GFF3031 at 463.389 kb on + strand, within GFF3031 at 463.390 kb on - strand, within GFF3031 at 463.498 kb on + strand, within GFF3031 at 463.499 kb on - strand, within GFF3031 at 463.499 kb on - strand, within GFF3031 at 463.499 kb on - strand, within GFF3031 at 463.509 kb on - strand, within GFF3031 at 463.572 kb on - strand, within GFF3031 at 463.572 kb on - strand, within GFF3031 at 463.572 kb on - strand, within GFF3031 at 463.573 kb on + strand, within GFF3031 at 463.574 kb on - strand, within GFF3031 at 463.574 kb on - strand, within GFF3031
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 460,578 + GFF3029 0.79 -1.1 460,578 + GFF3029 0.79 +0.3 460,579 - GFF3029 0.79 -2.2 460,579 - GFF3029 0.79 +0.5 460,602 + GFF3029 0.80 +0.4 460,603 - GFF3029 0.80 +0.5 460,603 - GFF3029 0.80 -0.4 460,603 - GFF3029 0.80 +0.6 460,615 - GFF3029 0.80 -0.2 461,053 - +0.3 461,564 + GFF3030 0.12 +0.6 462,314 + GFF3030 0.74 -2.4 462,315 - GFF3030 0.74 -0.2 462,315 - GFF3030 0.74 -0.3 462,315 - GFF3030 0.74 +0.6 462,315 - GFF3030 0.74 -1.5 462,318 + GFF3030 0.74 -0.4 462,318 + GFF3030 0.74 +0.2 462,319 - GFF3030 0.74 +1.0 462,319 - GFF3030 0.74 -0.4 462,437 + GFF3030 0.84 -0.2 462,438 - GFF3030 0.84 -0.4 462,438 - GFF3030 0.84 -0.8 462,438 - GFF3030 0.84 -0.7 462,438 - GFF3030 0.84 -1.4 462,464 + GFF3030 0.86 -1.2 462,501 - GFF3030 0.90 -0.7 462,694 + +0.1 462,694 + +0.3 462,699 + +1.0 462,699 + -0.0 462,699 + +0.5 462,699 + -0.9 462,716 + +0.3 462,716 + -0.4 462,716 + +0.2 462,716 + +0.3 462,716 + +0.6 462,717 - +0.5 462,717 - -2.6 462,717 - -0.2 462,717 - -0.0 462,717 - -0.3 462,717 - -0.9 462,731 + +0.6 462,732 - +0.9 462,732 - -1.2 462,732 - +1.6 462,732 - -0.1 462,748 + -0.9 462,748 + -0.6 462,748 + +0.5 462,748 + -2.5 462,748 + -1.1 462,749 - -0.2 462,749 - +1.6 462,749 - -0.2 462,749 - -0.7 462,820 + -0.3 462,821 - -1.0 462,821 - -0.1 462,821 - -0.1 462,821 - -0.5 462,822 - -1.1 462,838 - -0.2 462,865 + GFF3031 0.12 -1.7 462,997 - GFF3031 0.21 -0.2 463,040 + GFF3031 0.24 -0.2 463,040 + GFF3031 0.24 +0.0 463,041 - GFF3031 0.24 -0.9 463,041 - GFF3031 0.24 -0.2 463,231 + GFF3031 0.37 +1.1 463,232 - GFF3031 0.37 +0.4 463,385 + GFF3031 0.48 -0.8 463,385 + GFF3031 0.48 -0.1 463,385 + GFF3031 0.48 -0.4 463,385 + GFF3031 0.48 +0.0 463,386 - GFF3031 0.48 -0.5 463,386 - GFF3031 0.48 -1.2 463,386 - GFF3031 0.48 +0.1 463,386 - GFF3031 0.48 -0.4 463,386 - GFF3031 0.48 -1.5 463,389 + GFF3031 0.49 -0.0 463,389 + GFF3031 0.49 +0.7 463,389 + GFF3031 0.49 -1.0 463,390 - GFF3031 0.49 +0.0 463,498 + GFF3031 0.56 -0.8 463,499 - GFF3031 0.56 -0.3 463,499 - GFF3031 0.56 +0.6 463,499 - GFF3031 0.56 -2.2 463,509 - GFF3031 0.57 +0.1 463,572 - GFF3031 0.61 -0.3 463,572 - GFF3031 0.61 -1.5 463,572 - GFF3031 0.61 -0.7 463,573 + GFF3031 0.62 -0.8 463,574 - GFF3031 0.62 +0.3 463,574 - GFF3031 0.62 -0.5
Or see this region's nucleotide sequence