Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF284 and GFF285 are separated by 45 nucleotides GFF285 and GFF286 are separated by 111 nucleotides GFF286 and GFF287 are separated by 2 nucleotides
GFF284 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 319,256 to 320,149
GFF284
GFF285 - no description, at 320,195 to 321,037
GFF285
GFF286 - Transcriptional regulator, LysR family, at 321,149 to 322,042
GFF286
GFF287 - hypothetical protein, at 322,045 to 323,301
GFF287
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.210 kb on - strand at 320.340 kb on + strand, within GFF285 at 320.340 kb on + strand, within GFF285 at 320.341 kb on - strand, within GFF285 at 320.772 kb on + strand, within GFF285 at 320.773 kb on - strand, within GFF285 at 320.773 kb on - strand, within GFF285 at 320.773 kb on - strand, within GFF285 at 320.773 kb on - strand, within GFF285 at 321.052 kb on + strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.086 kb on - strand at 321.086 kb on - strand at 321.086 kb on - strand at 321.170 kb on + strand at 321.170 kb on + strand at 321.171 kb on - strand at 321.171 kb on - strand at 321.171 kb on - strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.282 kb on + strand, within GFF286 at 321.282 kb on + strand, within GFF286 at 321.282 kb on + strand, within GFF286 at 321.282 kb on + strand, within GFF286 at 321.283 kb on - strand, within GFF286 at 321.283 kb on - strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.488 kb on + strand, within GFF286 at 321.489 kb on - strand, within GFF286 at 321.489 kb on - strand, within GFF286 at 321.489 kb on - strand, within GFF286 at 321.490 kb on - strand, within GFF286 at 321.638 kb on + strand, within GFF286 at 321.638 kb on + strand, within GFF286 at 321.638 kb on + strand, within GFF286 at 321.639 kb on - strand, within GFF286 at 321.764 kb on + strand, within GFF286 at 321.765 kb on - strand, within GFF286 at 321.923 kb on + strand, within GFF286 at 321.923 kb on + strand, within GFF286 at 321.923 kb on + strand, within GFF286 at 321.924 kb on - strand, within GFF286 at 321.924 kb on - strand, within GFF286 at 321.924 kb on - strand, within GFF286 at 321.962 kb on + strand at 321.962 kb on + strand at 321.962 kb on + strand at 321.963 kb on - strand at 321.963 kb on - strand at 322.046 kb on + strand at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.442 kb on - strand, within GFF287 at 322.568 kb on + strand, within GFF287 at 322.569 kb on - strand, within GFF287 at 322.569 kb on - strand, within GFF287 at 322.719 kb on - strand, within GFF287
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 320,210 - +0.4 320,340 + GFF285 0.17 +1.4 320,340 + GFF285 0.17 +0.3 320,341 - GFF285 0.17 +0.0 320,772 + GFF285 0.68 -0.3 320,773 - GFF285 0.69 +0.4 320,773 - GFF285 0.69 -1.3 320,773 - GFF285 0.69 +0.4 320,773 - GFF285 0.69 -0.4 321,052 + -0.1 321,053 - -2.4 321,053 - +0.8 321,053 - +1.6 321,053 - +0.4 321,053 - +0.8 321,053 - -0.6 321,053 - -1.8 321,085 + +0.4 321,085 + +1.4 321,085 + -0.6 321,085 + -1.2 321,085 + -0.9 321,085 + -0.7 321,086 - +0.7 321,086 - -0.3 321,086 - -1.3 321,170 + -1.4 321,170 + +1.7 321,171 - +1.4 321,171 - -1.6 321,171 - +0.5 321,224 + +0.2 321,224 + -0.4 321,224 + +1.5 321,224 + +0.1 321,224 + -1.1 321,224 + -0.3 321,224 + +1.1 321,225 - -0.1 321,225 - -0.7 321,225 - -1.8 321,225 - +0.1 321,225 - -0.6 321,225 - -0.7 321,225 - +0.4 321,225 - +0.4 321,225 - +1.4 321,282 + GFF286 0.15 -1.4 321,282 + GFF286 0.15 +0.1 321,282 + GFF286 0.15 -0.2 321,282 + GFF286 0.15 -1.0 321,283 - GFF286 0.15 -0.0 321,283 - GFF286 0.15 -0.5 321,314 + GFF286 0.18 -0.9 321,314 + GFF286 0.18 -1.2 321,314 + GFF286 0.18 +1.6 321,314 + GFF286 0.18 -0.5 321,488 + GFF286 0.38 +0.5 321,489 - GFF286 0.38 -0.6 321,489 - GFF286 0.38 -0.3 321,489 - GFF286 0.38 -0.2 321,490 - GFF286 0.38 +1.9 321,638 + GFF286 0.55 -0.0 321,638 + GFF286 0.55 -0.6 321,638 + GFF286 0.55 -0.2 321,639 - GFF286 0.55 -0.3 321,764 + GFF286 0.69 -0.6 321,765 - GFF286 0.69 -0.3 321,923 + GFF286 0.87 -0.2 321,923 + GFF286 0.87 +0.5 321,923 + GFF286 0.87 -1.2 321,924 - GFF286 0.87 +0.6 321,924 - GFF286 0.87 -0.6 321,924 - GFF286 0.87 -0.7 321,962 + +0.8 321,962 + +0.7 321,962 + -0.2 321,963 - -0.7 321,963 - -0.4 322,046 + -1.6 322,265 + GFF287 0.18 +0.1 322,265 + GFF287 0.18 -1.5 322,265 + GFF287 0.18 -0.4 322,265 + GFF287 0.18 +2.6 322,265 + GFF287 0.18 +0.4 322,265 + GFF287 0.18 -1.6 322,266 - GFF287 0.18 -2.2 322,266 - GFF287 0.18 -0.9 322,266 - GFF287 0.18 -1.3 322,266 - GFF287 0.18 -0.8 322,266 - GFF287 0.18 -0.5 322,266 - GFF287 0.18 -1.1 322,266 - GFF287 0.18 +0.6 322,266 - GFF287 0.18 +1.4 322,266 - GFF287 0.18 -1.3 322,266 - GFF287 0.18 -1.1 322,442 - GFF287 0.32 +0.6 322,568 + GFF287 0.42 +0.0 322,569 - GFF287 0.42 -0.5 322,569 - GFF287 0.42 -0.1 322,719 - GFF287 0.54 +0.0
Or see this region's nucleotide sequence