Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2714 and GFF2715 are separated by 124 nucleotides GFF2715 and GFF2716 are separated by 5 nucleotides GFF2716 and GFF2717 are separated by 19 nucleotides
GFF2714 - 2-ketogluconate utilization repressor PtxS, at 147,649 to 148,674
GFF2714
GFF2715 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43), at 148,799 to 149,782
GFF2715
GFF2716 - 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45), at 149,788 to 150,741
GFF2716
GFF2717 - Epimerase KguE, at 150,761 to 151,510
GFF2717
Position (kb) 148
149
150 Strain fitness (log2 ratio) -2
-1
0
1 at 148.115 kb on + strand, within GFF2714 at 148.116 kb on - strand, within GFF2714 at 148.116 kb on - strand, within GFF2714 at 148.116 kb on - strand, within GFF2714 at 148.389 kb on - strand, within GFF2714 at 148.389 kb on - strand, within GFF2714 at 148.524 kb on - strand, within GFF2714 at 148.524 kb on - strand, within GFF2714 at 148.818 kb on + strand at 149.262 kb on + strand, within GFF2715 at 149.262 kb on + strand, within GFF2715 at 149.262 kb on + strand, within GFF2715 at 149.263 kb on - strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.545 kb on - strand, within GFF2715 at 150.023 kb on + strand, within GFF2716 at 150.023 kb on + strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.026 kb on + strand, within GFF2716 at 150.414 kb on - strand, within GFF2716 at 150.414 kb on - strand, within GFF2716 at 150.729 kb on + strand at 150.729 kb on + strand at 150.729 kb on + strand at 150.762 kb on + strand at 150.763 kb on - strand at 150.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 148,115 + GFF2714 0.45 -1.0 148,116 - GFF2714 0.46 +0.4 148,116 - GFF2714 0.46 -1.0 148,116 - GFF2714 0.46 -0.1 148,389 - GFF2714 0.72 -0.6 148,389 - GFF2714 0.72 +0.2 148,524 - GFF2714 0.85 -0.5 148,524 - GFF2714 0.85 +0.3 148,818 + -0.5 149,262 + GFF2715 0.47 -0.4 149,262 + GFF2715 0.47 -0.3 149,262 + GFF2715 0.47 -1.2 149,263 - GFF2715 0.47 -0.3 149,544 + GFF2715 0.76 +0.7 149,544 + GFF2715 0.76 -1.5 149,544 + GFF2715 0.76 +0.0 149,545 - GFF2715 0.76 +0.6 150,023 + GFF2716 0.25 +0.2 150,023 + GFF2716 0.25 +0.8 150,024 - GFF2716 0.25 -1.0 150,024 - GFF2716 0.25 -1.8 150,024 - GFF2716 0.25 +0.8 150,026 + GFF2716 0.25 -0.3 150,414 - GFF2716 0.66 +0.8 150,414 - GFF2716 0.66 -0.8 150,729 + -1.2 150,729 + +0.7 150,729 + -0.7 150,762 + +0.7 150,763 - +0.5 150,763 - +0.0
Or see this region's nucleotide sequence