Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2641 and GFF2642 overlap by 14 nucleotides GFF2642 and GFF2643 are separated by 62 nucleotides GFF2643 and GFF2644 are separated by 34 nucleotides GFF2644 and GFF2645 are separated by 14 nucleotides GFF2645 and GFF2646 are separated by 13 nucleotides
GFF2641 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 55,687 to 56,538
GFF2641
GFF2642 - Enoyl-CoA hydratase (EC 4.2.1.17), at 56,525 to 57,313
GFF2642
GFF2643 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 57,376 to 58,206
GFF2643
GFF2644 - MaoC family protein, at 58,241 to 58,660
GFF2644
GFF2645 - Acyl dehydratase, at 58,675 to 59,115
GFF2645
GFF2646 - Acetyl-CoA acetyltransferase, at 59,129 to 60,313
GFF2646
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.717 kb on + strand, within GFF2642 at 56.868 kb on + strand, within GFF2642 at 56.868 kb on + strand, within GFF2642 at 56.868 kb on + strand, within GFF2642 at 56.868 kb on + strand, within GFF2642 at 56.868 kb on + strand, within GFF2642 at 56.869 kb on - strand, within GFF2642 at 56.869 kb on - strand, within GFF2642 at 56.869 kb on - strand, within GFF2642 at 56.869 kb on - strand, within GFF2642 at 57.113 kb on + strand, within GFF2642 at 57.113 kb on + strand, within GFF2642 at 57.113 kb on + strand, within GFF2642 at 57.113 kb on + strand, within GFF2642 at 57.113 kb on + strand, within GFF2642 at 57.113 kb on + strand, within GFF2642 at 57.114 kb on - strand, within GFF2642 at 57.180 kb on - strand, within GFF2642 at 57.269 kb on + strand at 57.269 kb on + strand at 57.269 kb on + strand at 57.269 kb on + strand at 57.269 kb on + strand at 57.270 kb on - strand at 57.281 kb on + strand at 57.282 kb on - strand at 57.316 kb on - strand at 57.387 kb on + strand at 57.388 kb on - strand at 57.425 kb on + strand at 57.452 kb on + strand at 57.452 kb on + strand at 57.452 kb on + strand at 57.452 kb on + strand at 57.452 kb on + strand at 57.453 kb on - strand at 57.453 kb on - strand at 57.453 kb on - strand at 57.480 kb on + strand, within GFF2643 at 57.480 kb on + strand, within GFF2643 at 57.711 kb on - strand, within GFF2643 at 57.711 kb on - strand, within GFF2643 at 57.711 kb on - strand, within GFF2643 at 57.841 kb on + strand, within GFF2643 at 57.881 kb on + strand, within GFF2643 at 57.882 kb on - strand, within GFF2643 at 57.882 kb on - strand, within GFF2643 at 57.926 kb on + strand, within GFF2643 at 57.927 kb on - strand, within GFF2643 at 57.962 kb on + strand, within GFF2643 at 58.525 kb on - strand, within GFF2644 at 58.644 kb on + strand at 58.645 kb on - strand at 58.645 kb on - strand at 58.784 kb on + strand, within GFF2645 at 58.784 kb on + strand, within GFF2645 at 58.844 kb on + strand, within GFF2645 at 58.844 kb on + strand, within GFF2645 at 58.844 kb on + strand, within GFF2645 at 58.845 kb on - strand, within GFF2645 at 59.007 kb on - strand, within GFF2645 at 59.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 56,717 + GFF2642 0.24 +0.7 56,868 + GFF2642 0.43 +0.0 56,868 + GFF2642 0.43 -0.1 56,868 + GFF2642 0.43 +0.2 56,868 + GFF2642 0.43 +0.2 56,868 + GFF2642 0.43 +0.2 56,869 - GFF2642 0.44 +0.4 56,869 - GFF2642 0.44 -0.2 56,869 - GFF2642 0.44 +1.2 56,869 - GFF2642 0.44 -1.0 57,113 + GFF2642 0.75 +0.0 57,113 + GFF2642 0.75 +0.4 57,113 + GFF2642 0.75 +1.0 57,113 + GFF2642 0.75 -0.5 57,113 + GFF2642 0.75 -0.8 57,113 + GFF2642 0.75 +0.2 57,114 - GFF2642 0.75 +1.1 57,180 - GFF2642 0.83 +0.2 57,269 + +0.1 57,269 + -0.1 57,269 + -0.6 57,269 + +0.6 57,269 + +0.0 57,270 - -0.5 57,281 + +0.2 57,282 - -0.7 57,316 - +1.8 57,387 + +0.4 57,388 - -0.5 57,425 + -1.0 57,452 + -1.7 57,452 + -0.4 57,452 + +0.2 57,452 + -0.2 57,452 + +0.3 57,453 - +0.3 57,453 - -1.0 57,453 - -0.3 57,480 + GFF2643 0.13 -2.6 57,480 + GFF2643 0.13 -0.4 57,711 - GFF2643 0.40 +0.2 57,711 - GFF2643 0.40 -1.4 57,711 - GFF2643 0.40 -0.4 57,841 + GFF2643 0.56 +0.2 57,881 + GFF2643 0.61 +0.9 57,882 - GFF2643 0.61 -0.5 57,882 - GFF2643 0.61 -2.1 57,926 + GFF2643 0.66 -2.5 57,927 - GFF2643 0.66 -0.2 57,962 + GFF2643 0.71 -2.5 58,525 - GFF2644 0.68 +1.2 58,644 + -0.5 58,645 - -0.4 58,645 - +0.4 58,784 + GFF2645 0.25 -0.5 58,784 + GFF2645 0.25 -1.0 58,844 + GFF2645 0.38 -1.0 58,844 + GFF2645 0.38 -1.2 58,844 + GFF2645 0.38 +0.2 58,845 - GFF2645 0.39 -0.2 59,007 - GFF2645 0.75 -0.4 59,143 - -0.1
Or see this region's nucleotide sequence