Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2121 and GFF2122 are separated by 5 nucleotides GFF2122 and GFF2123 are separated by 27 nucleotides GFF2123 and GFF2124 are separated by 36 nucleotides
GFF2121 - Type IV fimbrial biogenesis protein PilY1, at 169,825 to 173,256
GFF2121
GFF2122 - Type IV pilus biogenesis protein PilE, at 173,262 to 173,714
GFF2122
GFF2123 - Copper metallochaperone PCu(A)C, inserts Cu(I) into cytochrome oxidase subunit II, at 173,742 to 174,218
GFF2123
GFF2124 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, thiol-disulfide reductase involved in Cu(I) insertion into CoxII Cu(A) center, at 174,255 to 174,872
GFF2124
Position (kb)
173
174 Strain fitness (log2 ratio)
-2
-1
0
1 at 172.324 kb on + strand, within GFF2121 at 172.324 kb on + strand, within GFF2121 at 172.324 kb on + strand, within GFF2121 at 172.325 kb on - strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.366 kb on + strand, within GFF2121 at 172.367 kb on - strand, within GFF2121 at 172.367 kb on - strand, within GFF2121 at 172.367 kb on - strand, within GFF2121 at 172.373 kb on - strand, within GFF2121 at 172.603 kb on + strand, within GFF2121 at 172.603 kb on + strand, within GFF2121 at 172.604 kb on - strand, within GFF2121 at 172.604 kb on - strand, within GFF2121 at 172.604 kb on - strand, within GFF2121 at 172.604 kb on - strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.627 kb on + strand, within GFF2121 at 172.628 kb on - strand, within GFF2121 at 172.628 kb on - strand, within GFF2121 at 172.628 kb on - strand, within GFF2121 at 172.628 kb on - strand, within GFF2121 at 172.687 kb on + strand, within GFF2121 at 172.688 kb on - strand, within GFF2121 at 172.688 kb on - strand, within GFF2121 at 172.688 kb on - strand, within GFF2121 at 172.900 kb on + strand, within GFF2121 at 172.900 kb on + strand, within GFF2121 at 172.900 kb on + strand, within GFF2121 at 172.900 kb on + strand, within GFF2121 at 172.900 kb on + strand, within GFF2121 at 172.901 kb on - strand, within GFF2121 at 172.901 kb on - strand, within GFF2121 at 172.901 kb on - strand, within GFF2121 at 173.064 kb on + strand at 173.077 kb on + strand at 173.077 kb on + strand at 173.077 kb on + strand at 173.078 kb on - strand at 173.156 kb on - strand at 173.289 kb on - strand at 173.289 kb on - strand at 173.289 kb on - strand at 173.383 kb on - strand, within GFF2122 at 173.383 kb on - strand, within GFF2122 at 173.383 kb on - strand, within GFF2122 at 173.460 kb on + strand, within GFF2122 at 173.461 kb on - strand, within GFF2122 at 173.474 kb on + strand, within GFF2122 at 173.475 kb on - strand, within GFF2122 at 173.475 kb on - strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.481 kb on + strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.482 kb on - strand, within GFF2122 at 173.485 kb on + strand, within GFF2122 at 173.486 kb on - strand, within GFF2122 at 173.537 kb on + strand, within GFF2122 at 173.538 kb on - strand, within GFF2122 at 173.538 kb on - strand, within GFF2122 at 173.538 kb on - strand, within GFF2122 at 173.556 kb on + strand, within GFF2122 at 173.557 kb on - strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.649 kb on + strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.650 kb on - strand, within GFF2122 at 173.882 kb on - strand, within GFF2123 at 173.918 kb on - strand, within GFF2123 at 173.918 kb on - strand, within GFF2123 at 174.286 kb on - strand at 174.361 kb on + strand, within GFF2124 at 174.361 kb on + strand, within GFF2124 at 174.361 kb on + strand, within GFF2124 at 174.362 kb on - strand, within GFF2124 at 174.388 kb on + strand, within GFF2124 at 174.416 kb on - strand, within GFF2124 at 174.416 kb on - strand, within GFF2124 at 174.467 kb on - strand, within GFF2124 at 174.502 kb on + strand, within GFF2124
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 172,324 + GFF2121 0.73 +0.8 172,324 + GFF2121 0.73 -0.8 172,324 + GFF2121 0.73 -1.0 172,325 - GFF2121 0.73 +1.6 172,366 + GFF2121 0.74 -0.7 172,366 + GFF2121 0.74 -1.5 172,366 + GFF2121 0.74 +1.1 172,366 + GFF2121 0.74 +0.2 172,366 + GFF2121 0.74 +0.5 172,366 + GFF2121 0.74 -0.6 172,366 + GFF2121 0.74 -0.4 172,367 - GFF2121 0.74 +0.0 172,367 - GFF2121 0.74 +0.3 172,367 - GFF2121 0.74 +0.8 172,373 - GFF2121 0.74 -0.1 172,603 + GFF2121 0.81 -0.4 172,603 + GFF2121 0.81 -0.5 172,604 - GFF2121 0.81 -0.3 172,604 - GFF2121 0.81 +0.4 172,604 - GFF2121 0.81 +1.1 172,604 - GFF2121 0.81 -0.8 172,627 + GFF2121 0.82 -0.2 172,627 + GFF2121 0.82 -0.4 172,627 + GFF2121 0.82 +0.1 172,627 + GFF2121 0.82 -0.7 172,627 + GFF2121 0.82 -0.4 172,627 + GFF2121 0.82 +0.4 172,627 + GFF2121 0.82 +0.8 172,627 + GFF2121 0.82 +1.0 172,627 + GFF2121 0.82 +0.7 172,628 - GFF2121 0.82 -0.0 172,628 - GFF2121 0.82 +0.1 172,628 - GFF2121 0.82 +1.0 172,628 - GFF2121 0.82 -1.5 172,687 + GFF2121 0.83 +0.0 172,688 - GFF2121 0.83 -2.4 172,688 - GFF2121 0.83 +0.2 172,688 - GFF2121 0.83 +0.8 172,900 + GFF2121 0.90 +0.3 172,900 + GFF2121 0.90 -0.3 172,900 + GFF2121 0.90 +0.8 172,900 + GFF2121 0.90 -0.2 172,900 + GFF2121 0.90 -0.7 172,901 - GFF2121 0.90 +0.4 172,901 - GFF2121 0.90 -1.5 172,901 - GFF2121 0.90 -0.2 173,064 + +0.5 173,077 + +0.6 173,077 + +0.3 173,077 + -0.5 173,078 - -0.5 173,156 - -0.6 173,289 - -1.5 173,289 - -0.5 173,289 - -0.5 173,383 - GFF2122 0.27 +0.4 173,383 - GFF2122 0.27 +1.1 173,383 - GFF2122 0.27 -0.3 173,460 + GFF2122 0.44 +0.1 173,461 - GFF2122 0.44 +0.8 173,474 + GFF2122 0.47 -1.2 173,475 - GFF2122 0.47 +0.7 173,475 - GFF2122 0.47 +0.3 173,481 + GFF2122 0.48 -1.0 173,481 + GFF2122 0.48 +0.3 173,481 + GFF2122 0.48 -0.4 173,481 + GFF2122 0.48 -0.2 173,481 + GFF2122 0.48 -0.6 173,481 + GFF2122 0.48 +0.7 173,481 + GFF2122 0.48 +0.4 173,481 + GFF2122 0.48 -1.3 173,481 + GFF2122 0.48 -0.5 173,481 + GFF2122 0.48 +0.2 173,481 + GFF2122 0.48 +0.4 173,481 + GFF2122 0.48 -0.6 173,481 + GFF2122 0.48 +0.1 173,482 - GFF2122 0.49 +0.8 173,482 - GFF2122 0.49 -0.2 173,482 - GFF2122 0.49 +0.1 173,482 - GFF2122 0.49 +1.1 173,482 - GFF2122 0.49 +0.8 173,482 - GFF2122 0.49 -0.3 173,482 - GFF2122 0.49 +1.4 173,482 - GFF2122 0.49 -1.2 173,482 - GFF2122 0.49 -0.3 173,482 - GFF2122 0.49 -0.7 173,482 - GFF2122 0.49 -1.5 173,482 - GFF2122 0.49 +0.6 173,482 - GFF2122 0.49 -0.2 173,482 - GFF2122 0.49 -1.0 173,482 - GFF2122 0.49 +0.1 173,482 - GFF2122 0.49 -0.8 173,482 - GFF2122 0.49 -0.5 173,482 - GFF2122 0.49 +0.8 173,482 - GFF2122 0.49 +0.1 173,482 - GFF2122 0.49 -1.2 173,482 - GFF2122 0.49 +1.3 173,482 - GFF2122 0.49 -1.0 173,482 - GFF2122 0.49 -0.3 173,485 + GFF2122 0.49 -0.0 173,486 - GFF2122 0.49 +0.1 173,537 + GFF2122 0.61 +0.8 173,538 - GFF2122 0.61 -1.2 173,538 - GFF2122 0.61 +0.1 173,538 - GFF2122 0.61 -0.2 173,556 + GFF2122 0.65 +0.8 173,557 - GFF2122 0.65 +0.1 173,649 + GFF2122 0.85 -0.1 173,649 + GFF2122 0.85 +1.7 173,649 + GFF2122 0.85 +0.0 173,649 + GFF2122 0.85 +0.3 173,649 + GFF2122 0.85 +0.5 173,649 + GFF2122 0.85 +1.1 173,649 + GFF2122 0.85 -0.9 173,650 - GFF2122 0.86 -0.4 173,650 - GFF2122 0.86 -1.0 173,650 - GFF2122 0.86 -0.5 173,650 - GFF2122 0.86 +0.5 173,650 - GFF2122 0.86 -0.5 173,650 - GFF2122 0.86 -0.9 173,650 - GFF2122 0.86 -1.3 173,650 - GFF2122 0.86 +0.1 173,882 - GFF2123 0.29 -1.0 173,918 - GFF2123 0.37 -1.4 173,918 - GFF2123 0.37 -0.4 174,286 - -0.2 174,361 + GFF2124 0.17 +0.1 174,361 + GFF2124 0.17 -1.5 174,361 + GFF2124 0.17 -0.1 174,362 - GFF2124 0.17 +0.5 174,388 + GFF2124 0.22 -0.4 174,416 - GFF2124 0.26 -0.5 174,416 - GFF2124 0.26 -1.0 174,467 - GFF2124 0.34 -0.4 174,502 + GFF2124 0.40 +0.1
Or see this region's nucleotide sequence