Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2087 and GFF2088 overlap by 4 nucleotides GFF2088 and GFF2089 are separated by 27 nucleotides GFF2089 and GFF2090 are separated by 85 nucleotides
GFF2087 - Ser/Thr and Tyr protein phosphatase (dual specificity), at 134,074 to 135,423
GFF2087
GFF2088 - Putative lipase in cluster with Phosphatidate cytidylyltransferase, at 135,420 to 137,186
GFF2088
GFF2089 - CDP-alcohol phosphatidyltransferase family protein, at 137,214 to 137,837
GFF2089
GFF2090 - Holliday junction ATP-dependent DNA helicase RuvB (EC 3.6.4.12), at 137,923 to 138,987
GFF2090
Position (kb)
135
136
137
138 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.643 kb on - strand, within GFF2087 at 134.747 kb on + strand, within GFF2087 at 134.748 kb on - strand, within GFF2087 at 134.785 kb on - strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.007 kb on - strand, within GFF2087 at 135.218 kb on + strand, within GFF2087 at 135.218 kb on + strand, within GFF2087 at 135.219 kb on - strand, within GFF2087 at 135.240 kb on - strand, within GFF2087 at 135.268 kb on + strand, within GFF2087 at 135.269 kb on - strand, within GFF2087 at 135.341 kb on + strand at 135.341 kb on + strand at 135.341 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.625 kb on + strand, within GFF2088 at 135.626 kb on - strand, within GFF2088 at 135.710 kb on - strand, within GFF2088 at 135.920 kb on - strand, within GFF2088 at 135.920 kb on - strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 136.000 kb on - strand, within GFF2088 at 136.057 kb on + strand, within GFF2088 at 136.058 kb on - strand, within GFF2088 at 136.111 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.219 kb on + strand, within GFF2088 at 136.624 kb on + strand, within GFF2088 at 136.624 kb on + strand, within GFF2088 at 136.625 kb on - strand, within GFF2088 at 136.625 kb on - strand, within GFF2088 at 136.672 kb on + strand, within GFF2088 at 136.673 kb on - strand, within GFF2088 at 136.673 kb on - strand, within GFF2088 at 136.741 kb on + strand, within GFF2088 at 136.742 kb on - strand, within GFF2088 at 136.742 kb on - strand, within GFF2088 at 136.742 kb on - strand, within GFF2088 at 136.798 kb on + strand, within GFF2088 at 136.798 kb on + strand, within GFF2088 at 137.116 kb on + strand at 137.116 kb on + strand at 137.117 kb on - strand at 137.210 kb on + strand at 137.211 kb on - strand at 137.211 kb on - strand at 137.211 kb on - strand at 137.276 kb on + strand at 137.353 kb on + strand, within GFF2089 at 137.353 kb on + strand, within GFF2089 at 137.353 kb on + strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.531 kb on - strand, within GFF2089 at 137.531 kb on - strand, within GFF2089 at 137.827 kb on + strand at 137.861 kb on - strand at 137.861 kb on - strand at 137.892 kb on + strand at 137.892 kb on + strand at 137.893 kb on - strand at 137.895 kb on + strand at 137.895 kb on + strand at 137.895 kb on + strand at 137.903 kb on + strand at 137.928 kb on + strand at 138.040 kb on + strand, within GFF2090 at 138.041 kb on - strand, within GFF2090
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 134,642 + GFF2087 0.42 +0.1 134,642 + GFF2087 0.42 -0.4 134,642 + GFF2087 0.42 -2.8 134,642 + GFF2087 0.42 -0.6 134,643 - GFF2087 0.42 -0.3 134,747 + GFF2087 0.50 -0.4 134,748 - GFF2087 0.50 +0.0 134,785 - GFF2087 0.53 +0.1 135,006 + GFF2087 0.69 -0.2 135,006 + GFF2087 0.69 +0.0 135,007 - GFF2087 0.69 -0.1 135,218 + GFF2087 0.85 +0.8 135,218 + GFF2087 0.85 -1.5 135,219 - GFF2087 0.85 +0.2 135,240 - GFF2087 0.86 -0.2 135,268 + GFF2087 0.88 +0.3 135,269 - GFF2087 0.89 -0.0 135,341 + +0.2 135,341 + +1.6 135,341 + -1.5 135,350 + -1.0 135,350 + -0.6 135,350 + +2.8 135,350 + +0.0 135,351 - +0.2 135,351 - +0.1 135,351 - +0.0 135,351 - +1.1 135,351 - -0.8 135,625 + GFF2088 0.12 -1.3 135,626 - GFF2088 0.12 -0.2 135,710 - GFF2088 0.16 -0.7 135,920 - GFF2088 0.28 +0.0 135,920 - GFF2088 0.28 +0.4 135,999 + GFF2088 0.33 -0.5 135,999 + GFF2088 0.33 +0.5 135,999 + GFF2088 0.33 -0.6 136,000 - GFF2088 0.33 +0.7 136,057 + GFF2088 0.36 +1.0 136,058 - GFF2088 0.36 -0.6 136,111 + GFF2088 0.39 +1.2 136,117 + GFF2088 0.39 -2.7 136,117 + GFF2088 0.39 -0.8 136,117 + GFF2088 0.39 -1.0 136,219 + GFF2088 0.45 -0.3 136,624 + GFF2088 0.68 -1.1 136,624 + GFF2088 0.68 +0.4 136,625 - GFF2088 0.68 -1.7 136,625 - GFF2088 0.68 -0.0 136,672 + GFF2088 0.71 -1.0 136,673 - GFF2088 0.71 +0.7 136,673 - GFF2088 0.71 +1.0 136,741 + GFF2088 0.75 -0.6 136,742 - GFF2088 0.75 -0.7 136,742 - GFF2088 0.75 -0.7 136,742 - GFF2088 0.75 -2.3 136,798 + GFF2088 0.78 -1.0 136,798 + GFF2088 0.78 -0.0 137,116 + +0.0 137,116 + -0.2 137,117 - +0.5 137,210 + -0.4 137,211 - +1.3 137,211 - +0.7 137,211 - +0.6 137,276 + -0.0 137,353 + GFF2089 0.22 -2.2 137,353 + GFF2089 0.22 -0.3 137,353 + GFF2089 0.22 -0.6 137,354 - GFF2089 0.22 +0.1 137,354 - GFF2089 0.22 -1.3 137,354 - GFF2089 0.22 -0.0 137,354 - GFF2089 0.22 -0.4 137,531 - GFF2089 0.51 -0.1 137,531 - GFF2089 0.51 -0.3 137,827 + +0.8 137,861 - -0.5 137,861 - +1.3 137,892 + +0.1 137,892 + -0.1 137,893 - +0.0 137,895 + -0.6 137,895 + -1.3 137,895 + -0.0 137,903 + -0.7 137,928 + -0.4 138,040 + GFF2090 0.11 +0.2 138,041 - GFF2090 0.11 +0.3
Or see this region's nucleotide sequence