Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1672 and GFF1673 are separated by 30 nucleotides GFF1673 and GFF1674 overlap by 4 nucleotides GFF1674 and GFF1675 overlap by 8 nucleotides GFF1675 and GFF1676 overlap by 4 nucleotides
GFF1672 - BUG/TctC family periplasmic protein, at 317,860 to 318,825
GFF1672
GFF1673 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 318,856 to 319,566
GFF1673
GFF1674 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 319,563 to 320,324
GFF1674
GFF1675 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 320,317 to 321,297
GFF1675
GFF1676 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 321,294 to 322,166
GFF1676
Position (kb) 319
320
321 Strain fitness (log2 ratio) -1
0
1 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 319.203 kb on + strand, within GFF1673 at 319.621 kb on + strand at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.597 kb on - strand, within GFF1675 at 320.601 kb on - strand, within GFF1675 at 320.601 kb on - strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.619 kb on - strand, within GFF1675 at 320.619 kb on - strand, within GFF1675 at 321.089 kb on + strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.182 kb on + strand, within GFF1675 at 321.183 kb on - strand, within GFF1675 at 321.183 kb on - strand, within GFF1675
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 318,947 + GFF1673 0.13 -0.1 318,947 + GFF1673 0.13 +1.3 318,947 + GFF1673 0.13 -0.7 319,203 + GFF1673 0.49 +0.2 319,621 + +0.8 320,423 + GFF1675 0.11 +0.1 320,423 + GFF1675 0.11 -0.5 320,423 + GFF1675 0.11 -0.0 320,423 + GFF1675 0.11 -0.4 320,423 + GFF1675 0.11 +0.3 320,597 - GFF1675 0.29 -0.9 320,601 - GFF1675 0.29 +0.5 320,601 - GFF1675 0.29 +0.2 320,618 + GFF1675 0.31 +0.8 320,618 + GFF1675 0.31 -0.1 320,618 + GFF1675 0.31 +0.8 320,619 - GFF1675 0.31 +0.5 320,619 - GFF1675 0.31 +0.4 321,089 + GFF1675 0.79 -0.2 321,090 - GFF1675 0.79 -0.2 321,090 - GFF1675 0.79 -0.2 321,090 - GFF1675 0.79 -0.8 321,182 + GFF1675 0.88 -0.7 321,183 - GFF1675 0.88 +0.2 321,183 - GFF1675 0.88 -0.9
Or see this region's nucleotide sequence