Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1663 and GFF1664 overlap by 4 nucleotides
GFF1663 - Isocitrate lyase (EC 4.1.3.1), at 307,106 to 307,972
GFF1663
GFF1664 - redox proteins related to the succinate dehydrogenases and fumarate reductases, at 307,969 to 309,384
GFF1664
Position (kb) 307
308 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 306.111 kb on + strand at 306.111 kb on + strand at 306.111 kb on + strand at 306.111 kb on + strand at 306.111 kb on + strand at 306.111 kb on + strand at 306.111 kb on + strand at 306.111 kb on + strand at 306.112 kb on - strand at 306.112 kb on - strand at 306.112 kb on - strand at 306.112 kb on - strand at 306.112 kb on - strand at 306.274 kb on + strand at 306.274 kb on + strand at 306.275 kb on - strand at 306.275 kb on - strand at 306.488 kb on - strand at 306.539 kb on + strand at 306.694 kb on + strand at 306.694 kb on + strand at 306.694 kb on + strand at 306.844 kb on + strand at 307.116 kb on + strand at 307.117 kb on - strand at 307.230 kb on + strand, within GFF1663 at 307.231 kb on + strand, within GFF1663 at 307.231 kb on - strand, within GFF1663 at 307.231 kb on - strand, within GFF1663 at 307.233 kb on + strand, within GFF1663 at 307.233 kb on + strand, within GFF1663 at 307.458 kb on + strand, within GFF1663 at 307.833 kb on + strand, within GFF1663 at 307.857 kb on + strand, within GFF1663 at 307.858 kb on - strand, within GFF1663 at 307.908 kb on + strand at 307.908 kb on + strand at 307.908 kb on + strand at 307.909 kb on - strand at 307.909 kb on - strand at 307.909 kb on - strand at 307.957 kb on - strand at 308.066 kb on + strand at 308.067 kb on - strand at 308.225 kb on + strand, within GFF1664 at 308.437 kb on + strand, within GFF1664 at 308.437 kb on + strand, within GFF1664 at 308.437 kb on + strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 308.438 kb on - strand, within GFF1664
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 306,111 + +0.3 306,111 + -0.1 306,111 + +1.0 306,111 + -0.2 306,111 + -0.3 306,111 + -0.3 306,111 + +1.1 306,111 + +0.1 306,112 - -0.8 306,112 - +0.8 306,112 - +0.6 306,112 - -0.0 306,112 - -0.3 306,274 + -0.6 306,274 + -0.2 306,275 - -0.2 306,275 - +0.4 306,488 - -0.5 306,539 + -0.3 306,694 + +1.0 306,694 + -1.9 306,694 + -0.3 306,844 + +0.1 307,116 + -0.0 307,117 - -0.2 307,230 + GFF1663 0.14 -0.2 307,231 + GFF1663 0.14 -0.3 307,231 - GFF1663 0.14 +0.3 307,231 - GFF1663 0.14 -1.0 307,233 + GFF1663 0.15 +0.1 307,233 + GFF1663 0.15 +0.4 307,458 + GFF1663 0.41 -0.3 307,833 + GFF1663 0.84 -0.7 307,857 + GFF1663 0.87 -1.2 307,858 - GFF1663 0.87 -0.4 307,908 + -0.8 307,908 + -1.6 307,908 + -1.5 307,909 - +0.5 307,909 - -1.4 307,909 - +3.4 307,957 - -1.1 308,066 + -0.1 308,067 - -0.8 308,225 + GFF1664 0.18 +0.4 308,437 + GFF1664 0.33 -0.8 308,437 + GFF1664 0.33 +0.1 308,437 + GFF1664 0.33 +1.5 308,438 - GFF1664 0.33 +0.6 308,438 - GFF1664 0.33 -0.1 308,438 - GFF1664 0.33 -1.3 308,438 - GFF1664 0.33 +0.8
Or see this region's nucleotide sequence