Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1599 and GFF1600 overlap by 4 nucleotides GFF1600 and GFF1601 are separated by 19 nucleotides GFF1601 and GFF1602 are separated by 23 nucleotides GFF1602 and GFF1603 overlap by 4 nucleotides
GFF1599 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 197,145 to 198,116
GFF1599
GFF1600 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 198,113 to 199,000
GFF1600
GFF1601 - no description, at 199,020 to 200,219
GFF1601
GFF1602 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 200,243 to 200,965
GFF1602
GFF1603 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 200,962 to 201,729
GFF1603
Position (kb) 199
200
201 Strain fitness (log2 ratio) -2
-1
0
1
2 at 198.073 kb on + strand at 198.073 kb on + strand at 198.074 kb on - strand at 198.074 kb on - strand at 198.074 kb on - strand at 198.106 kb on + strand at 198.106 kb on + strand at 198.107 kb on - strand at 198.107 kb on - strand at 198.107 kb on - strand at 198.204 kb on + strand, within GFF1600 at 198.715 kb on + strand, within GFF1600 at 198.715 kb on + strand, within GFF1600 at 199.066 kb on + strand at 199.303 kb on + strand, within GFF1601 at 199.303 kb on + strand, within GFF1601 at 199.318 kb on + strand, within GFF1601 at 199.318 kb on + strand, within GFF1601 at 199.319 kb on - strand, within GFF1601 at 199.319 kb on - strand, within GFF1601 at 199.363 kb on + strand, within GFF1601 at 199.363 kb on + strand, within GFF1601 at 199.364 kb on - strand, within GFF1601 at 199.364 kb on - strand, within GFF1601 at 199.403 kb on - strand, within GFF1601 at 199.487 kb on - strand, within GFF1601 at 199.499 kb on - strand, within GFF1601 at 199.512 kb on + strand, within GFF1601 at 199.534 kb on + strand, within GFF1601 at 199.535 kb on - strand, within GFF1601 at 199.615 kb on + strand, within GFF1601 at 199.675 kb on + strand, within GFF1601 at 199.675 kb on + strand, within GFF1601 at 199.676 kb on - strand, within GFF1601 at 199.795 kb on + strand, within GFF1601 at 199.796 kb on - strand, within GFF1601 at 199.903 kb on + strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 200.213 kb on + strand at 200.652 kb on + strand, within GFF1602 at 200.652 kb on + strand, within GFF1602 at 200.985 kb on - strand at 200.985 kb on - strand at 201.042 kb on - strand, within GFF1603
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 198,073 + -0.5 198,073 + -1.5 198,074 - -0.1 198,074 - -0.1 198,074 - -0.7 198,106 + -0.2 198,106 + -1.0 198,107 - -0.6 198,107 - -0.8 198,107 - +1.4 198,204 + GFF1600 0.10 -1.1 198,715 + GFF1600 0.68 +1.0 198,715 + GFF1600 0.68 +0.6 199,066 + -0.3 199,303 + GFF1601 0.24 +2.4 199,303 + GFF1601 0.24 +1.5 199,318 + GFF1601 0.25 +0.0 199,318 + GFF1601 0.25 +0.2 199,319 - GFF1601 0.25 -0.7 199,319 - GFF1601 0.25 +0.4 199,363 + GFF1601 0.29 +0.1 199,363 + GFF1601 0.29 +0.5 199,364 - GFF1601 0.29 +0.0 199,364 - GFF1601 0.29 +0.2 199,403 - GFF1601 0.32 +2.4 199,487 - GFF1601 0.39 +1.2 199,499 - GFF1601 0.40 -0.9 199,512 + GFF1601 0.41 -0.2 199,534 + GFF1601 0.43 +0.8 199,535 - GFF1601 0.43 -0.4 199,615 + GFF1601 0.50 +0.3 199,675 + GFF1601 0.55 -1.3 199,675 + GFF1601 0.55 -0.0 199,676 - GFF1601 0.55 -1.1 199,795 + GFF1601 0.65 +0.4 199,796 - GFF1601 0.65 +0.2 199,903 + GFF1601 0.74 +1.4 199,904 - GFF1601 0.74 +0.4 199,904 - GFF1601 0.74 -1.4 199,904 - GFF1601 0.74 -1.3 200,213 + +0.1 200,652 + GFF1602 0.57 -2.2 200,652 + GFF1602 0.57 -0.6 200,985 - +0.7 200,985 - -0.8 201,042 - GFF1603 0.10 -0.8
Or see this region's nucleotide sequence