Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF122 and GFF123 overlap by 8 nucleotides GFF123 and GFF124 are separated by 16 nucleotides GFF124 and GFF125 overlap by 4 nucleotides
GFF122 - Dihydroorotate dehydrogenase electron transfer subunit (EC 1.3.3.1), at 150,815 to 151,690
GFF122
GFF123 - Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit (EC 1.3.1.14), at 151,683 to 152,633
GFF123
GFF124 - Beta-ureidopropionase (EC 3.5.1.6), at 152,650 to 153,930
GFF124
GFF125 - Dihydroorotase (EC 3.5.2.3), at 153,927 to 154,979
GFF125
Position (kb) 152
153
154 Strain fitness (log2 ratio) -1
0
1 at 151.764 kb on + strand at 151.915 kb on - strand, within GFF123 at 151.917 kb on + strand, within GFF123 at 151.941 kb on + strand, within GFF123 at 152.115 kb on + strand, within GFF123 at 152.115 kb on + strand, within GFF123 at 152.116 kb on - strand, within GFF123 at 152.368 kb on - strand, within GFF123 at 152.481 kb on - strand, within GFF123 at 152.527 kb on - strand, within GFF123 at 152.759 kb on - strand at 152.789 kb on - strand, within GFF124 at 152.907 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.233 kb on - strand, within GFF124 at 153.233 kb on - strand, within GFF124 at 153.590 kb on - strand, within GFF124 at 154.108 kb on - strand, within GFF125 at 154.596 kb on + strand, within GFF125 at 154.596 kb on + strand, within GFF125 at 154.596 kb on + strand, within GFF125 at 154.770 kb on + strand, within GFF125 at 154.771 kb on - strand, within GFF125
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 151,764 + -1.0 151,915 - GFF123 0.24 +0.8 151,917 + GFF123 0.25 +0.1 151,941 + GFF123 0.27 -0.1 152,115 + GFF123 0.45 +0.6 152,115 + GFF123 0.45 -0.7 152,116 - GFF123 0.46 -0.2 152,368 - GFF123 0.72 +0.3 152,481 - GFF123 0.84 +0.1 152,527 - GFF123 0.89 +0.3 152,759 - -0.8 152,789 - GFF124 0.11 +0.5 152,907 + GFF124 0.20 +0.0 153,067 + GFF124 0.33 +0.9 153,067 + GFF124 0.33 +0.6 153,067 + GFF124 0.33 -0.3 153,068 - GFF124 0.33 +0.0 153,068 - GFF124 0.33 +1.1 153,068 - GFF124 0.33 +0.4 153,068 - GFF124 0.33 -1.4 153,233 - GFF124 0.46 -1.1 153,233 - GFF124 0.46 +0.9 153,590 - GFF124 0.73 +0.0 154,108 - GFF125 0.17 +0.4 154,596 + GFF125 0.64 -0.5 154,596 + GFF125 0.64 -0.2 154,596 + GFF125 0.64 -1.6 154,770 + GFF125 0.80 +0.0 154,771 - GFF125 0.80 +0.2
Or see this region's nucleotide sequence