Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1106 and GFF1107 overlap by 4 nucleotides GFF1107 and GFF1108 are separated by 367 nucleotides
GFF1106 - UvrD/REP helicase, at 420,691 to 423,951
GFF1106
GFF1107 - NAD(FAD)-utilizing dehydrogenases, at 423,948 to 425,186
GFF1107
GFF1108 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 425,554 to 427,425
GFF1108
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.259 kb on + strand, within GFF1106 at 423.259 kb on + strand, within GFF1106 at 423.260 kb on - strand, within GFF1106 at 423.887 kb on - strand at 423.938 kb on + strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.045 kb on - strand at 424.045 kb on - strand at 424.045 kb on - strand at 424.045 kb on - strand at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.338 kb on - strand, within GFF1107 at 424.542 kb on - strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.705 kb on - strand, within GFF1107 at 424.705 kb on - strand, within GFF1107 at 424.877 kb on + strand, within GFF1107 at 424.877 kb on + strand, within GFF1107 at 424.878 kb on - strand, within GFF1107 at 424.878 kb on - strand, within GFF1107 at 425.115 kb on + strand at 425.116 kb on - strand at 425.166 kb on - strand at 425.184 kb on + strand at 425.184 kb on + strand at 425.184 kb on + strand at 425.184 kb on + strand at 425.185 kb on - strand at 425.185 kb on - strand at 425.185 kb on - strand at 425.382 kb on + strand at 425.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 423,259 + GFF1106 0.79 -0.8 423,259 + GFF1106 0.79 -0.5 423,260 - GFF1106 0.79 -0.1 423,887 - +0.4 423,938 + +0.5 424,013 - +0.1 424,013 - -0.0 424,013 - -0.1 424,013 - -0.5 424,013 - -0.1 424,044 + -1.1 424,044 + +0.1 424,044 + -0.6 424,044 + +0.2 424,044 + -0.1 424,044 + -0.7 424,045 - +1.5 424,045 - +0.5 424,045 - -1.3 424,045 - +0.1 424,337 + GFF1107 0.31 -0.2 424,337 + GFF1107 0.31 -0.1 424,337 + GFF1107 0.31 -0.4 424,337 + GFF1107 0.31 +0.6 424,337 + GFF1107 0.31 +1.2 424,338 - GFF1107 0.31 -0.6 424,542 - GFF1107 0.48 -0.1 424,704 + GFF1107 0.61 +0.1 424,704 + GFF1107 0.61 +0.3 424,704 + GFF1107 0.61 -0.3 424,704 + GFF1107 0.61 +0.2 424,704 + GFF1107 0.61 -2.5 424,704 + GFF1107 0.61 +0.3 424,704 + GFF1107 0.61 +0.3 424,705 - GFF1107 0.61 -0.3 424,705 - GFF1107 0.61 +0.2 424,877 + GFF1107 0.75 -1.4 424,877 + GFF1107 0.75 -0.1 424,878 - GFF1107 0.75 +0.7 424,878 - GFF1107 0.75 -0.3 425,115 + +0.4 425,116 - +0.0 425,166 - +0.1 425,184 + -0.8 425,184 + -0.3 425,184 + -0.5 425,184 + -1.2 425,185 - -1.1 425,185 - -0.7 425,185 - +2.5 425,382 + +0.2 425,383 - -0.4
Or see this region's nucleotide sequence