Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1062 and GFF1063 are separated by 41 nucleotides GFF1063 and GFF1064 are separated by 75 nucleotides GFF1064 and GFF1065 are separated by 59 nucleotides GFF1065 and GFF1066 overlap by 4 nucleotides
GFF1062 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 375,686 to 376,462
GFF1062
GFF1063 - dioxygenase, TauD/TfdA family, at 376,504 to 377,343
GFF1063
GFF1064 - Transcriptional regulator, GntR family, at 377,419 to 378,153
GFF1064
GFF1065 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 378,213 to 379,046
GFF1065
GFF1066 - Aspartate aminotransferase (EC 2.6.1.1), at 379,043 to 380,218
GFF1066
Position (kb) 377
378
379 Strain fitness (log2 ratio) -1
0
1
2 at 376.729 kb on + strand, within GFF1063 at 376.741 kb on + strand, within GFF1063 at 376.741 kb on + strand, within GFF1063 at 376.822 kb on + strand, within GFF1063 at 376.823 kb on - strand, within GFF1063 at 376.823 kb on - strand, within GFF1063 at 376.823 kb on - strand, within GFF1063 at 376.976 kb on - strand, within GFF1063 at 376.976 kb on - strand, within GFF1063 at 376.976 kb on - strand, within GFF1063 at 376.984 kb on + strand, within GFF1063 at 376.985 kb on - strand, within GFF1063 at 376.988 kb on - strand, within GFF1063 at 377.101 kb on + strand, within GFF1063 at 377.101 kb on + strand, within GFF1063 at 377.102 kb on - strand, within GFF1063 at 377.102 kb on - strand, within GFF1063 at 377.206 kb on + strand, within GFF1063 at 377.861 kb on - strand, within GFF1064 at 377.893 kb on + strand, within GFF1064 at 377.893 kb on + strand, within GFF1064 at 377.894 kb on - strand, within GFF1064 at 377.894 kb on - strand, within GFF1064 at 377.914 kb on + strand, within GFF1064 at 377.914 kb on + strand, within GFF1064 at 377.915 kb on - strand, within GFF1064 at 377.915 kb on - strand, within GFF1064 at 377.915 kb on - strand, within GFF1064 at 377.959 kb on + strand, within GFF1064 at 377.960 kb on - strand, within GFF1064 at 377.968 kb on + strand, within GFF1064 at 377.968 kb on + strand, within GFF1064 at 378.242 kb on - strand at 378.375 kb on + strand, within GFF1065 at 378.375 kb on + strand, within GFF1065 at 378.376 kb on - strand, within GFF1065 at 378.558 kb on + strand, within GFF1065 at 378.558 kb on + strand, within GFF1065 at 378.559 kb on - strand, within GFF1065 at 378.583 kb on - strand, within GFF1065 at 378.660 kb on + strand, within GFF1065 at 378.661 kb on - strand, within GFF1065 at 378.661 kb on - strand, within GFF1065 at 378.859 kb on - strand, within GFF1065 at 378.859 kb on - strand, within GFF1065 at 378.933 kb on + strand, within GFF1065 at 378.934 kb on - strand, within GFF1065
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 376,729 + GFF1063 0.27 +0.1 376,741 + GFF1063 0.28 -0.1 376,741 + GFF1063 0.28 +1.5 376,822 + GFF1063 0.38 -0.4 376,823 - GFF1063 0.38 +0.1 376,823 - GFF1063 0.38 -0.2 376,823 - GFF1063 0.38 +0.7 376,976 - GFF1063 0.56 +1.8 376,976 - GFF1063 0.56 -0.2 376,976 - GFF1063 0.56 -0.3 376,984 + GFF1063 0.57 -0.9 376,985 - GFF1063 0.57 -0.1 376,988 - GFF1063 0.58 -0.4 377,101 + GFF1063 0.71 -0.6 377,101 + GFF1063 0.71 +0.6 377,102 - GFF1063 0.71 -0.5 377,102 - GFF1063 0.71 +1.2 377,206 + GFF1063 0.84 -0.5 377,861 - GFF1064 0.60 +1.3 377,893 + GFF1064 0.64 +0.2 377,893 + GFF1064 0.64 -0.9 377,894 - GFF1064 0.65 -0.2 377,894 - GFF1064 0.65 -0.2 377,914 + GFF1064 0.67 +0.7 377,914 + GFF1064 0.67 -0.1 377,915 - GFF1064 0.67 +1.0 377,915 - GFF1064 0.67 -0.3 377,915 - GFF1064 0.67 -0.3 377,959 + GFF1064 0.73 +2.4 377,960 - GFF1064 0.74 -0.1 377,968 + GFF1064 0.75 +0.3 377,968 + GFF1064 0.75 -0.2 378,242 - -1.3 378,375 + GFF1065 0.19 -1.1 378,375 + GFF1065 0.19 -1.1 378,376 - GFF1065 0.20 -0.9 378,558 + GFF1065 0.41 -0.6 378,558 + GFF1065 0.41 +0.4 378,559 - GFF1065 0.41 -0.5 378,583 - GFF1065 0.44 -0.2 378,660 + GFF1065 0.54 -0.3 378,661 - GFF1065 0.54 -0.6 378,661 - GFF1065 0.54 -1.1 378,859 - GFF1065 0.77 -0.7 378,859 - GFF1065 0.77 +0.8 378,933 + GFF1065 0.86 -0.1 378,934 - GFF1065 0.86 -0.1
Or see this region's nucleotide sequence