Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF845 and GFF846 overlap by 4 nucleotides GFF846 and GFF847 are separated by 16 nucleotides GFF847 and GFF848 are separated by 19 nucleotides GFF848 and GFF849 are separated by 143 nucleotides
GFF845 - dTDP-glucose 4,6-dehydratase, at 154,752 to 155,495
GFF845
GFF846 - hypothetical protein, at 155,492 to 156,025
GFF846
GFF847 - Acetyltransferase, at 156,042 to 156,548
GFF847
GFF848 - no description, at 156,568 to 157,011
GFF848
GFF849 - putative carboxylesterase, at 157,155 to 158,909
GFF849
Position (kb) 156
157 Strain fitness (log2 ratio) -2
-1
0
1
2 at 155.130 kb on + strand, within GFF845 at 155.131 kb on - strand, within GFF845 at 155.131 kb on - strand, within GFF845 at 156.070 kb on + strand at 156.071 kb on - strand at 156.121 kb on + strand, within GFF847 at 156.121 kb on + strand, within GFF847 at 156.122 kb on - strand, within GFF847 at 156.122 kb on - strand, within GFF847 at 156.122 kb on - strand, within GFF847 at 156.181 kb on + strand, within GFF847 at 156.182 kb on - strand, within GFF847 at 156.184 kb on + strand, within GFF847 at 156.185 kb on - strand, within GFF847 at 156.615 kb on - strand, within GFF848 at 156.656 kb on + strand, within GFF848 at 156.656 kb on + strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.690 kb on + strand, within GFF848 at 156.691 kb on - strand, within GFF848 at 156.768 kb on - strand, within GFF848 at 157.296 kb on + strand at 157.518 kb on + strand, within GFF849 at 157.518 kb on + strand, within GFF849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 155,130 + GFF845 0.51 -0.1 155,131 - GFF845 0.51 +0.4 155,131 - GFF845 0.51 -1.4 156,070 + -0.5 156,071 - +1.7 156,121 + GFF847 0.16 -1.2 156,121 + GFF847 0.16 -1.4 156,122 - GFF847 0.16 +0.2 156,122 - GFF847 0.16 -0.6 156,122 - GFF847 0.16 +0.5 156,181 + GFF847 0.27 -0.6 156,182 - GFF847 0.28 -0.2 156,184 + GFF847 0.28 +0.5 156,185 - GFF847 0.28 +0.1 156,615 - GFF848 0.11 -0.4 156,656 + GFF848 0.20 -1.7 156,656 + GFF848 0.20 -1.6 156,657 - GFF848 0.20 -2.5 156,657 - GFF848 0.20 -1.2 156,657 - GFF848 0.20 -0.5 156,657 - GFF848 0.20 +0.6 156,690 + GFF848 0.27 +1.8 156,691 - GFF848 0.28 -1.1 156,768 - GFF848 0.45 -1.6 157,296 + -0.7 157,518 + GFF849 0.21 +0.3 157,518 + GFF849 0.21 -1.3
Or see this region's nucleotide sequence