Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF746 and GFF747 are separated by 35 nucleotides GFF747 and GFF748 are separated by 15 nucleotides GFF748 and GFF749 are separated by 2 nucleotides
GFF746 - BUG/TctC family periplasmic protein, at 9,413 to 10,393
GFF746
GFF747 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 10,429 to 11,733
GFF747
GFF748 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase (EC 4.1.3.17), at 11,749 to 12,447
GFF748
GFF749 - 4-oxalomesaconate hydratase (EC 4.2.1.83), at 12,450 to 13,478
GFF749
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 9.454 kb on - strand at 9.630 kb on + strand, within GFF746 at 9.834 kb on + strand, within GFF746 at 9.835 kb on - strand, within GFF746 at 9.835 kb on - strand, within GFF746 at 10.060 kb on - strand, within GFF746 at 10.122 kb on + strand, within GFF746 at 10.122 kb on + strand, within GFF746 at 10.123 kb on - strand, within GFF746 at 10.123 kb on - strand, within GFF746 at 10.123 kb on - strand, within GFF746 at 10.123 kb on - strand, within GFF746 at 10.386 kb on + strand at 10.497 kb on - strand at 10.562 kb on + strand, within GFF747 at 10.562 kb on + strand, within GFF747 at 10.562 kb on + strand, within GFF747 at 10.562 kb on + strand, within GFF747 at 10.562 kb on + strand, within GFF747 at 10.563 kb on - strand, within GFF747 at 10.563 kb on - strand, within GFF747 at 10.563 kb on - strand, within GFF747 at 10.585 kb on + strand, within GFF747 at 10.586 kb on - strand, within GFF747 at 10.629 kb on - strand, within GFF747 at 10.629 kb on - strand, within GFF747 at 10.778 kb on + strand, within GFF747 at 10.812 kb on - strand, within GFF747 at 10.910 kb on + strand, within GFF747 at 10.910 kb on + strand, within GFF747 at 10.911 kb on - strand, within GFF747 at 10.911 kb on - strand, within GFF747 at 10.911 kb on - strand, within GFF747 at 10.911 kb on - strand, within GFF747 at 10.946 kb on + strand, within GFF747 at 10.946 kb on + strand, within GFF747 at 10.946 kb on + strand, within GFF747 at 10.946 kb on + strand, within GFF747 at 10.946 kb on + strand, within GFF747 at 10.946 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.949 kb on + strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 10.950 kb on - strand, within GFF747 at 11.204 kb on + strand, within GFF747 at 11.244 kb on - strand, within GFF747 at 11.531 kb on + strand, within GFF747 at 11.531 kb on + strand, within GFF747 at 11.532 kb on - strand, within GFF747 at 11.532 kb on - strand, within GFF747 at 11.567 kb on + strand, within GFF747 at 11.567 kb on + strand, within GFF747 at 11.568 kb on - strand, within GFF747 at 11.568 kb on - strand, within GFF747 at 11.759 kb on + strand at 11.760 kb on - strand at 11.760 kb on - strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 12.515 kb on - strand at 12.515 kb on - strand at 12.515 kb on - strand at 12.562 kb on + strand, within GFF749 at 12.563 kb on - strand, within GFF749 at 12.563 kb on - strand, within GFF749 at 12.580 kb on + strand, within GFF749 at 12.694 kb on + strand, within GFF749 at 12.695 kb on - strand, within GFF749 at 12.695 kb on - strand, within GFF749 at 12.695 kb on - strand, within GFF749
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 9,454 - -0.4 9,630 + GFF746 0.22 +0.4 9,834 + GFF746 0.43 +0.3 9,835 - GFF746 0.43 -0.8 9,835 - GFF746 0.43 -0.3 10,060 - GFF746 0.66 -0.1 10,122 + GFF746 0.72 -0.1 10,122 + GFF746 0.72 -0.9 10,123 - GFF746 0.72 -1.5 10,123 - GFF746 0.72 +0.3 10,123 - GFF746 0.72 -0.8 10,123 - GFF746 0.72 -2.6 10,386 + +0.3 10,497 - +0.7 10,562 + GFF747 0.10 +0.1 10,562 + GFF747 0.10 -0.1 10,562 + GFF747 0.10 -0.3 10,562 + GFF747 0.10 -0.6 10,562 + GFF747 0.10 +1.4 10,563 - GFF747 0.10 -0.5 10,563 - GFF747 0.10 -1.4 10,563 - GFF747 0.10 -1.0 10,585 + GFF747 0.12 +0.3 10,586 - GFF747 0.12 -0.9 10,629 - GFF747 0.15 -1.1 10,629 - GFF747 0.15 -0.4 10,778 + GFF747 0.27 +0.1 10,812 - GFF747 0.29 +0.8 10,910 + GFF747 0.37 -0.2 10,910 + GFF747 0.37 -1.2 10,911 - GFF747 0.37 +0.0 10,911 - GFF747 0.37 -0.7 10,911 - GFF747 0.37 -1.0 10,911 - GFF747 0.37 -0.8 10,946 + GFF747 0.40 -1.0 10,946 + GFF747 0.40 +1.0 10,946 + GFF747 0.40 -0.5 10,946 + GFF747 0.40 -1.3 10,946 + GFF747 0.40 -1.6 10,946 + GFF747 0.40 -0.1 10,949 + GFF747 0.40 -0.8 10,949 + GFF747 0.40 -0.4 10,949 + GFF747 0.40 -0.5 10,949 + GFF747 0.40 +0.8 10,949 + GFF747 0.40 +0.1 10,949 + GFF747 0.40 -0.5 10,949 + GFF747 0.40 +0.1 10,949 + GFF747 0.40 -0.4 10,949 + GFF747 0.40 +0.0 10,950 - GFF747 0.40 +1.2 10,950 - GFF747 0.40 -1.0 10,950 - GFF747 0.40 +0.0 10,950 - GFF747 0.40 -1.2 10,950 - GFF747 0.40 -1.4 10,950 - GFF747 0.40 -0.4 10,950 - GFF747 0.40 -0.2 10,950 - GFF747 0.40 +0.0 10,950 - GFF747 0.40 -0.4 11,204 + GFF747 0.59 -0.6 11,244 - GFF747 0.62 +0.3 11,531 + GFF747 0.84 -0.9 11,531 + GFF747 0.84 -0.9 11,532 - GFF747 0.85 -0.5 11,532 - GFF747 0.85 -0.4 11,567 + GFF747 0.87 -0.2 11,567 + GFF747 0.87 +0.6 11,568 - GFF747 0.87 -1.8 11,568 - GFF747 0.87 -1.2 11,759 + -0.1 11,760 - +0.2 11,760 - -0.3 11,798 + -0.2 11,798 + -1.2 11,798 + -1.0 11,798 + -1.0 11,798 + +0.8 11,798 + -0.0 11,798 + -0.4 11,798 + -0.3 11,799 - +1.5 11,799 - -0.8 11,799 - -0.6 11,799 - -0.1 11,799 - +0.6 11,799 - +0.1 11,799 - +1.8 11,799 - -0.2 12,515 - +0.1 12,515 - +0.6 12,515 - -0.1 12,562 + GFF749 0.11 -0.1 12,563 - GFF749 0.11 +0.3 12,563 - GFF749 0.11 -0.6 12,580 + GFF749 0.13 -0.1 12,694 + GFF749 0.24 -0.7 12,695 - GFF749 0.24 -1.4 12,695 - GFF749 0.24 +0.6 12,695 - GFF749 0.24 -0.6
Or see this region's nucleotide sequence