Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7351 and GFF7352 are separated by 42 nucleotides GFF7352 and GFF7353 overlap by 7 nucleotides GFF7353 and GFF7354 overlap by 1 nucleotides
GFF7351 - 1,2-phenylacetyl-CoA epoxidase, subunit E (EC 1.14.13.149), at 490 to 1,635
GFF7351
GFF7352 - 1,2-phenylacetyl-CoA epoxidase, subunit D (EC 1.14.13.149), at 1,678 to 2,190
GFF7352
GFF7353 - 1,2-phenylacetyl-CoA epoxidase, subunit C (EC 1.14.13.149), at 2,184 to 2,945
GFF7353
GFF7354 - 1,2-phenylacetyl-CoA epoxidase, subunit B (EC 1.14.13.149), at 2,945 to 3,238
GFF7354
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 0.932 kb on - strand, within GFF7351 at 1.198 kb on - strand, within GFF7351 at 1.223 kb on + strand, within GFF7351 at 1.223 kb on + strand, within GFF7351 at 1.223 kb on + strand, within GFF7351 at 1.224 kb on - strand, within GFF7351 at 1.397 kb on + strand, within GFF7351 at 1.445 kb on + strand, within GFF7351 at 1.617 kb on + strand at 1.694 kb on + strand at 1.694 kb on + strand at 1.695 kb on - strand at 1.695 kb on - strand at 1.716 kb on + strand at 1.716 kb on + strand at 2.016 kb on - strand, within GFF7352 at 2.203 kb on + strand at 2.362 kb on - strand, within GFF7353 at 2.395 kb on + strand, within GFF7353 at 2.437 kb on + strand, within GFF7353 at 2.437 kb on + strand, within GFF7353 at 2.437 kb on + strand, within GFF7353 at 2.437 kb on + strand, within GFF7353 at 2.437 kb on + strand, within GFF7353 at 2.438 kb on - strand, within GFF7353 at 2.438 kb on - strand, within GFF7353 at 2.611 kb on + strand, within GFF7353 at 2.612 kb on - strand, within GFF7353 at 2.632 kb on + strand, within GFF7353 at 2.777 kb on - strand, within GFF7353 at 2.777 kb on - strand, within GFF7353 at 2.915 kb on - strand at 2.976 kb on + strand, within GFF7354 at 2.976 kb on + strand, within GFF7354 at 2.976 kb on + strand, within GFF7354 at 2.994 kb on + strand, within GFF7354 at 2.994 kb on + strand, within GFF7354 at 3.111 kb on + strand, within GFF7354 at 3.111 kb on + strand, within GFF7354
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 932 - GFF7351 0.39 -0.7 1,198 - GFF7351 0.62 -0.5 1,223 + GFF7351 0.64 -0.1 1,223 + GFF7351 0.64 +1.0 1,223 + GFF7351 0.64 -0.3 1,224 - GFF7351 0.64 -1.8 1,397 + GFF7351 0.79 -0.3 1,445 + GFF7351 0.83 -0.8 1,617 + -0.9 1,694 + +1.6 1,694 + -1.2 1,695 - +0.1 1,695 - +0.1 1,716 + +1.5 1,716 + +0.9 2,016 - GFF7352 0.66 +0.1 2,203 + -0.4 2,362 - GFF7353 0.23 -0.5 2,395 + GFF7353 0.28 +1.7 2,437 + GFF7353 0.33 +0.5 2,437 + GFF7353 0.33 -1.0 2,437 + GFF7353 0.33 -0.4 2,437 + GFF7353 0.33 -1.3 2,437 + GFF7353 0.33 +0.9 2,438 - GFF7353 0.33 -0.1 2,438 - GFF7353 0.33 +0.8 2,611 + GFF7353 0.56 -1.9 2,612 - GFF7353 0.56 +0.3 2,632 + GFF7353 0.59 +0.9 2,777 - GFF7353 0.78 -0.0 2,777 - GFF7353 0.78 +0.9 2,915 - +3.7 2,976 + GFF7354 0.11 -0.0 2,976 + GFF7354 0.11 -0.5 2,976 + GFF7354 0.11 +1.2 2,994 + GFF7354 0.17 -0.7 2,994 + GFF7354 0.17 -0.2 3,111 + GFF7354 0.56 +0.3 3,111 + GFF7354 0.56 -1.8
Or see this region's nucleotide sequence