Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7102 and GFF7103 overlap by 4 nucleotides GFF7103 and GFF7104 overlap by 4 nucleotides GFF7104 and GFF7105 overlap by 4 nucleotides
GFF7102 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 19,884 to 21,350
GFF7102
GFF7103 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 21,347 to 22,096
GFF7103
GFF7104 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 22,093 to 22,743
GFF7104
GFF7105 - Homoprotocatechuate degradative operon repressor, at 22,740 to 23,171
GFF7105
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 20.353 kb on + strand, within GFF7102 at 20.353 kb on + strand, within GFF7102 at 20.354 kb on - strand, within GFF7102 at 20.354 kb on - strand, within GFF7102 at 20.354 kb on - strand, within GFF7102 at 20.354 kb on - strand, within GFF7102 at 20.476 kb on + strand, within GFF7102 at 20.654 kb on + strand, within GFF7102 at 20.953 kb on + strand, within GFF7102 at 20.953 kb on + strand, within GFF7102 at 20.954 kb on - strand, within GFF7102 at 20.954 kb on - strand, within GFF7102 at 20.954 kb on - strand, within GFF7102 at 20.954 kb on - strand, within GFF7102 at 20.977 kb on + strand, within GFF7102 at 20.977 kb on + strand, within GFF7102 at 21.016 kb on + strand, within GFF7102 at 21.016 kb on + strand, within GFF7102 at 21.017 kb on - strand, within GFF7102 at 21.017 kb on - strand, within GFF7102 at 21.066 kb on + strand, within GFF7102 at 21.066 kb on + strand, within GFF7102 at 21.066 kb on + strand, within GFF7102 at 21.066 kb on + strand, within GFF7102 at 21.066 kb on + strand, within GFF7102 at 21.066 kb on + strand, within GFF7102 at 21.067 kb on - strand, within GFF7102 at 21.067 kb on - strand, within GFF7102 at 21.067 kb on - strand, within GFF7102 at 21.067 kb on - strand, within GFF7102 at 21.292 kb on + strand at 21.292 kb on + strand at 21.293 kb on - strand at 21.486 kb on + strand, within GFF7103 at 21.487 kb on - strand, within GFF7103 at 21.667 kb on - strand, within GFF7103 at 21.681 kb on + strand, within GFF7103 at 21.696 kb on + strand, within GFF7103 at 21.697 kb on - strand, within GFF7103 at 21.726 kb on + strand, within GFF7103 at 21.726 kb on + strand, within GFF7103 at 21.726 kb on + strand, within GFF7103 at 21.726 kb on + strand, within GFF7103 at 21.726 kb on + strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.727 kb on - strand, within GFF7103 at 21.732 kb on + strand, within GFF7103 at 21.732 kb on + strand, within GFF7103 at 21.732 kb on + strand, within GFF7103 at 21.732 kb on + strand, within GFF7103 at 21.732 kb on + strand, within GFF7103 at 21.732 kb on + strand, within GFF7103 at 21.733 kb on - strand, within GFF7103 at 21.733 kb on - strand, within GFF7103 at 21.733 kb on - strand, within GFF7103 at 21.733 kb on - strand, within GFF7103 at 21.733 kb on - strand, within GFF7103 at 21.795 kb on + strand, within GFF7103 at 21.796 kb on - strand, within GFF7103 at 21.796 kb on - strand, within GFF7103 at 21.796 kb on - strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.924 kb on + strand, within GFF7103 at 21.924 kb on + strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 22.203 kb on + strand, within GFF7104 at 22.334 kb on - strand, within GFF7104 at 22.439 kb on + strand, within GFF7104 at 22.440 kb on - strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.638 kb on - strand, within GFF7104 at 22.638 kb on - strand, within GFF7104 at 22.703 kb on + strand at 22.703 kb on + strand at 22.703 kb on + strand at 22.703 kb on + strand at 22.703 kb on + strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.707 kb on - strand at 22.766 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.828 kb on + strand, within GFF7105 at 22.828 kb on + strand, within GFF7105
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 20,353 + GFF7102 0.32 -0.2 20,353 + GFF7102 0.32 -1.2 20,354 - GFF7102 0.32 -0.1 20,354 - GFF7102 0.32 -0.5 20,354 - GFF7102 0.32 +0.9 20,354 - GFF7102 0.32 +1.3 20,476 + GFF7102 0.40 -0.4 20,654 + GFF7102 0.52 -0.6 20,953 + GFF7102 0.73 -0.6 20,953 + GFF7102 0.73 -2.0 20,954 - GFF7102 0.73 -1.1 20,954 - GFF7102 0.73 +0.3 20,954 - GFF7102 0.73 +0.3 20,954 - GFF7102 0.73 -0.4 20,977 + GFF7102 0.75 -0.3 20,977 + GFF7102 0.75 +0.7 21,016 + GFF7102 0.77 +2.2 21,016 + GFF7102 0.77 -0.4 21,017 - GFF7102 0.77 -0.9 21,017 - GFF7102 0.77 -0.8 21,066 + GFF7102 0.81 +0.4 21,066 + GFF7102 0.81 -0.4 21,066 + GFF7102 0.81 -1.2 21,066 + GFF7102 0.81 -0.3 21,066 + GFF7102 0.81 -1.1 21,066 + GFF7102 0.81 +0.4 21,067 - GFF7102 0.81 -0.6 21,067 - GFF7102 0.81 -0.7 21,067 - GFF7102 0.81 -1.3 21,067 - GFF7102 0.81 +0.4 21,292 + -1.3 21,292 + -0.7 21,293 - +0.1 21,486 + GFF7103 0.19 -0.4 21,487 - GFF7103 0.19 -0.8 21,667 - GFF7103 0.43 -0.2 21,681 + GFF7103 0.45 +0.0 21,696 + GFF7103 0.47 +1.5 21,697 - GFF7103 0.47 -0.2 21,726 + GFF7103 0.51 -1.4 21,726 + GFF7103 0.51 +0.1 21,726 + GFF7103 0.51 +0.6 21,726 + GFF7103 0.51 +0.0 21,726 + GFF7103 0.51 -0.3 21,727 - GFF7103 0.51 +0.6 21,727 - GFF7103 0.51 -0.9 21,727 - GFF7103 0.51 +0.2 21,727 - GFF7103 0.51 -0.4 21,727 - GFF7103 0.51 -0.4 21,727 - GFF7103 0.51 +0.7 21,727 - GFF7103 0.51 -0.6 21,727 - GFF7103 0.51 -1.0 21,727 - GFF7103 0.51 +1.1 21,732 + GFF7103 0.51 -0.2 21,732 + GFF7103 0.51 -1.8 21,732 + GFF7103 0.51 -0.7 21,732 + GFF7103 0.51 +0.3 21,732 + GFF7103 0.51 -0.5 21,732 + GFF7103 0.51 -0.2 21,733 - GFF7103 0.51 +0.6 21,733 - GFF7103 0.51 +0.1 21,733 - GFF7103 0.51 +0.0 21,733 - GFF7103 0.51 -0.5 21,733 - GFF7103 0.51 -0.7 21,795 + GFF7103 0.60 -0.1 21,796 - GFF7103 0.60 -1.7 21,796 - GFF7103 0.60 -1.0 21,796 - GFF7103 0.60 -0.5 21,804 + GFF7103 0.61 -0.4 21,804 + GFF7103 0.61 -0.3 21,804 + GFF7103 0.61 -0.5 21,804 + GFF7103 0.61 +0.1 21,804 + GFF7103 0.61 -1.0 21,804 + GFF7103 0.61 -1.7 21,804 + GFF7103 0.61 -1.1 21,804 + GFF7103 0.61 -1.2 21,804 + GFF7103 0.61 +0.9 21,804 + GFF7103 0.61 -1.1 21,804 + GFF7103 0.61 +0.2 21,804 + GFF7103 0.61 -0.1 21,804 + GFF7103 0.61 -2.2 21,804 + GFF7103 0.61 +0.4 21,805 - GFF7103 0.61 +0.3 21,805 - GFF7103 0.61 +1.3 21,805 - GFF7103 0.61 -0.6 21,805 - GFF7103 0.61 -1.0 21,805 - GFF7103 0.61 -1.0 21,805 - GFF7103 0.61 -1.1 21,805 - GFF7103 0.61 -0.5 21,805 - GFF7103 0.61 -0.8 21,805 - GFF7103 0.61 +0.2 21,805 - GFF7103 0.61 +0.6 21,805 - GFF7103 0.61 -1.1 21,805 - GFF7103 0.61 -0.1 21,924 + GFF7103 0.77 -0.8 21,924 + GFF7103 0.77 +0.3 21,925 - GFF7103 0.77 +1.4 21,925 - GFF7103 0.77 -0.2 21,925 - GFF7103 0.77 -0.8 21,925 - GFF7103 0.77 -1.2 21,925 - GFF7103 0.77 +0.9 21,925 - GFF7103 0.77 -0.0 22,203 + GFF7104 0.17 -0.8 22,334 - GFF7104 0.37 +0.3 22,439 + GFF7104 0.53 +0.3 22,440 - GFF7104 0.53 -0.5 22,481 + GFF7104 0.60 -1.6 22,481 + GFF7104 0.60 +0.2 22,481 + GFF7104 0.60 -1.2 22,481 + GFF7104 0.60 -0.4 22,481 + GFF7104 0.60 -0.2 22,481 + GFF7104 0.60 -1.3 22,482 - GFF7104 0.60 -0.2 22,482 - GFF7104 0.60 +0.8 22,482 - GFF7104 0.60 +0.3 22,482 - GFF7104 0.60 +0.3 22,482 - GFF7104 0.60 +0.0 22,482 - GFF7104 0.60 +0.2 22,482 - GFF7104 0.60 +0.4 22,607 + GFF7104 0.79 +2.5 22,607 + GFF7104 0.79 -1.8 22,607 + GFF7104 0.79 -0.4 22,607 + GFF7104 0.79 -2.3 22,607 + GFF7104 0.79 -0.4 22,607 + GFF7104 0.79 +0.4 22,608 - GFF7104 0.79 -2.3 22,608 - GFF7104 0.79 -0.2 22,608 - GFF7104 0.79 +1.5 22,608 - GFF7104 0.79 +1.4 22,637 + GFF7104 0.84 -0.5 22,637 + GFF7104 0.84 +0.6 22,637 + GFF7104 0.84 -2.3 22,637 + GFF7104 0.84 -0.8 22,638 - GFF7104 0.84 +0.1 22,638 - GFF7104 0.84 +0.7 22,703 + +0.4 22,703 + +0.8 22,703 + -0.2 22,703 + -0.1 22,703 + -0.7 22,704 - -1.8 22,704 - +0.2 22,704 - -1.1 22,704 - -0.2 22,704 - +0.2 22,704 - +0.9 22,705 + -0.8 22,705 + -0.1 22,705 + -0.4 22,705 + -0.2 22,705 + -1.1 22,705 + +0.0 22,705 + -0.0 22,705 + +0.5 22,705 + +0.2 22,705 + -1.8 22,705 + -0.2 22,705 + +0.1 22,705 + -0.3 22,705 + -0.0 22,705 + +1.0 22,705 + -1.0 22,705 + +0.3 22,705 + -0.6 22,705 + -0.4 22,705 + -1.7 22,705 + -0.2 22,705 + -0.5 22,705 + -0.9 22,705 + -1.4 22,705 + -1.4 22,705 + +0.6 22,705 + -0.5 22,706 - -0.7 22,706 - -1.6 22,706 - +1.1 22,706 - +0.1 22,706 - +0.1 22,706 - -0.3 22,706 - +0.1 22,706 - +0.2 22,706 - +0.3 22,706 - -0.5 22,706 - +0.9 22,706 - -0.8 22,706 - -0.3 22,706 - +0.5 22,706 - +0.2 22,706 - -0.7 22,706 - -0.1 22,706 - -0.3 22,706 - +0.1 22,706 - +0.1 22,707 - -0.9 22,766 + -0.5 22,780 + +0.1 22,780 + +0.2 22,780 + +0.4 22,780 + -0.6 22,780 + -0.1 22,780 + -0.3 22,780 + -1.0 22,780 + -0.3 22,780 + +0.5 22,780 + +0.1 22,780 + +3.5 22,780 + -1.0 22,781 - +0.0 22,781 - +0.4 22,781 - -1.3 22,781 - -0.1 22,781 - +0.2 22,781 - -1.0 22,781 - +0.7 22,781 - -0.4 22,781 - -0.6 22,781 - -0.5 22,781 - +0.4 22,781 - -0.4 22,828 + GFF7105 0.20 -0.3 22,828 + GFF7105 0.20 +0.4
Or see this region's nucleotide sequence