Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6794 and GFF6795 overlap by 4 nucleotides GFF6795 and GFF6796 are separated by 19 nucleotides GFF6796 and GFF6797 are separated by 194 nucleotides GFF6797 and GFF6798 overlap by 17 nucleotides
GFF6794 - hypothetical protein, at 42,198 to 43,739
GFF6794
GFF6795 - similar to puromycin N-acetyltransferase, at 43,736 to 44,224
GFF6795
GFF6796 - Predicted kinase, at 44,244 to 44,750
GFF6796
GFF6797 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 44,945 to 45,730
GFF6797
GFF6798 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 45,714 to 46,367
GFF6798
Position (kb) 44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 43.615 kb on - strand at 43.615 kb on - strand at 43.734 kb on + strand at 43.734 kb on + strand at 43.734 kb on + strand at 43.734 kb on + strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.737 kb on + strand at 43.738 kb on - strand at 43.758 kb on + strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.821 kb on + strand, within GFF6795 at 43.821 kb on + strand, within GFF6795 at 43.821 kb on + strand, within GFF6795 at 43.822 kb on - strand, within GFF6795 at 43.822 kb on - strand, within GFF6795 at 44.070 kb on + strand, within GFF6795 at 44.237 kb on + strand at 44.238 kb on - strand at 44.395 kb on - strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.600 kb on - strand, within GFF6796 at 44.712 kb on + strand at 44.712 kb on + strand at 44.712 kb on + strand at 44.713 kb on - strand at 44.713 kb on - strand at 44.854 kb on - strand at 44.919 kb on + strand at 45.643 kb on - strand, within GFF6797 at 45.643 kb on - strand, within GFF6797
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 43,615 - -0.8 43,615 - -2.9 43,734 + -0.4 43,734 + +0.7 43,734 + +0.8 43,734 + -0.0 43,735 - -1.6 43,735 - -0.2 43,735 - +0.7 43,735 - -0.4 43,735 - -0.8 43,735 - +0.9 43,735 - -0.5 43,737 + -0.4 43,738 - -0.5 43,758 + -0.1 43,759 - -0.5 43,759 - +0.2 43,759 - +0.6 43,759 - -0.9 43,759 - -0.4 43,821 + GFF6795 0.17 -0.1 43,821 + GFF6795 0.17 -1.1 43,821 + GFF6795 0.17 +0.6 43,822 - GFF6795 0.18 -0.4 43,822 - GFF6795 0.18 -1.0 44,070 + GFF6795 0.68 -0.1 44,237 + -0.0 44,238 - +1.3 44,395 - GFF6796 0.30 +0.4 44,569 + GFF6796 0.64 -1.1 44,569 + GFF6796 0.64 -1.8 44,569 + GFF6796 0.64 -0.4 44,569 + GFF6796 0.64 -1.4 44,569 + GFF6796 0.64 -0.4 44,569 + GFF6796 0.64 -0.8 44,570 - GFF6796 0.64 -0.5 44,570 - GFF6796 0.64 -0.4 44,570 - GFF6796 0.64 -0.9 44,570 - GFF6796 0.64 -0.0 44,570 - GFF6796 0.64 -0.3 44,570 - GFF6796 0.64 -1.4 44,600 - GFF6796 0.70 -0.1 44,712 + +1.2 44,712 + -0.0 44,712 + -0.9 44,713 - +0.1 44,713 - +0.1 44,854 - -1.7 44,919 + -0.3 45,643 - GFF6797 0.89 -0.5 45,643 - GFF6797 0.89 +0.8
Or see this region's nucleotide sequence