Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6425 and GFF6426 are separated by 275 nucleotides GFF6426 and GFF6427 are separated by 24 nucleotides GFF6427 and GFF6428 are separated by 48 nucleotides GFF6428 and GFF6429 overlap by 4 nucleotides
GFF6425 - no description, at 36 to 527
GFF6425
GFF6426 - no description, at 803 to 949
GFF6426
GFF6427 - ABC transporter, substrate-binding protein (cluster 7, di-/tri-carboxylate), at 974 to 1,939
GFF6427
GFF6428 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 1,988 to 2,368
GFF6428
GFF6429 - Pyruvate:Oxaloacetate transcarboxylase domain protein, at 2,365 to 3,348
GFF6429
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-1
0
1
2 at 0.074 kb on + strand at 0.206 kb on - strand, within GFF6425 at 0.445 kb on + strand, within GFF6425 at 0.445 kb on + strand, within GFF6425 at 0.446 kb on - strand, within GFF6425 at 0.592 kb on + strand at 0.592 kb on + strand at 0.663 kb on + strand at 0.663 kb on + strand at 0.752 kb on + strand at 0.752 kb on + strand at 0.948 kb on - strand at 1.065 kb on + strand at 1.065 kb on + strand at 1.065 kb on + strand at 1.065 kb on + strand at 1.065 kb on + strand at 1.065 kb on + strand at 1.066 kb on - strand at 1.066 kb on - strand at 1.209 kb on + strand, within GFF6427 at 1.237 kb on + strand, within GFF6427 at 1.237 kb on + strand, within GFF6427 at 1.237 kb on + strand, within GFF6427 at 1.237 kb on + strand, within GFF6427 at 1.238 kb on - strand, within GFF6427 at 1.238 kb on - strand, within GFF6427 at 1.238 kb on - strand, within GFF6427 at 1.277 kb on - strand, within GFF6427 at 1.278 kb on + strand, within GFF6427 at 1.278 kb on + strand, within GFF6427 at 1.279 kb on - strand, within GFF6427 at 1.279 kb on - strand, within GFF6427 at 1.395 kb on + strand, within GFF6427 at 1.395 kb on + strand, within GFF6427 at 1.395 kb on + strand, within GFF6427 at 1.396 kb on - strand, within GFF6427 at 1.482 kb on + strand, within GFF6427 at 1.482 kb on + strand, within GFF6427 at 1.482 kb on + strand, within GFF6427 at 1.483 kb on - strand, within GFF6427 at 1.483 kb on - strand, within GFF6427 at 1.483 kb on - strand, within GFF6427 at 1.483 kb on - strand, within GFF6427 at 1.659 kb on + strand, within GFF6427 at 1.668 kb on + strand, within GFF6427 at 1.669 kb on - strand, within GFF6427 at 1.686 kb on + strand, within GFF6427 at 1.687 kb on - strand, within GFF6427 at 2.041 kb on - strand, within GFF6428 at 2.041 kb on - strand, within GFF6428 at 2.304 kb on + strand, within GFF6428 at 2.304 kb on + strand, within GFF6428 at 2.304 kb on + strand, within GFF6428 at 2.304 kb on + strand, within GFF6428 at 2.342 kb on + strand at 2.342 kb on + strand at 2.343 kb on - strand at 2.372 kb on + strand at 2.372 kb on + strand at 2.793 kb on - strand, within GFF6429
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 74 + -0.6 206 - GFF6425 0.35 +0.5 445 + GFF6425 0.83 +0.0 445 + GFF6425 0.83 -0.2 446 - GFF6425 0.83 +0.4 592 + -0.4 592 + -1.1 663 + -1.1 663 + -1.3 752 + +1.6 752 + -0.7 948 - +0.3 1,065 + -0.0 1,065 + -0.2 1,065 + +0.7 1,065 + -0.2 1,065 + -0.4 1,065 + +0.0 1,066 - -0.2 1,066 - -0.7 1,209 + GFF6427 0.24 +0.5 1,237 + GFF6427 0.27 -0.5 1,237 + GFF6427 0.27 -0.8 1,237 + GFF6427 0.27 +0.0 1,237 + GFF6427 0.27 +0.3 1,238 - GFF6427 0.27 +1.6 1,238 - GFF6427 0.27 +1.2 1,238 - GFF6427 0.27 +1.0 1,277 - GFF6427 0.31 -1.0 1,278 + GFF6427 0.31 +0.8 1,278 + GFF6427 0.31 +0.7 1,279 - GFF6427 0.32 -1.4 1,279 - GFF6427 0.32 -0.2 1,395 + GFF6427 0.44 +0.3 1,395 + GFF6427 0.44 -0.4 1,395 + GFF6427 0.44 -0.3 1,396 - GFF6427 0.44 -0.1 1,482 + GFF6427 0.53 +0.5 1,482 + GFF6427 0.53 +0.9 1,482 + GFF6427 0.53 +0.3 1,483 - GFF6427 0.53 -0.2 1,483 - GFF6427 0.53 -0.4 1,483 - GFF6427 0.53 -0.3 1,483 - GFF6427 0.53 +0.0 1,659 + GFF6427 0.71 -0.1 1,668 + GFF6427 0.72 +1.4 1,669 - GFF6427 0.72 +0.8 1,686 + GFF6427 0.74 -0.3 1,687 - GFF6427 0.74 -0.8 2,041 - GFF6428 0.14 -1.5 2,041 - GFF6428 0.14 +0.2 2,304 + GFF6428 0.83 +1.2 2,304 + GFF6428 0.83 -1.7 2,304 + GFF6428 0.83 -1.1 2,304 + GFF6428 0.83 +0.9 2,342 + +2.3 2,342 + +0.0 2,343 - -0.3 2,372 + +0.3 2,372 + +0.6 2,793 - GFF6429 0.43 -0.2
Or see this region's nucleotide sequence