Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6200 and GFF6201 are separated by 64 nucleotides GFF6201 and GFF6202 are separated by 10 nucleotides GFF6202 and GFF6203 are separated by 151 nucleotides
GFF6200 - integral membrane sensor signal transduction histidine kinase, at 106,235 to 107,704
GFF6200
GFF6201 - Oxidoreductase, aldo/keto reductase family, at 107,769 to 108,593
GFF6201
GFF6202 - Unknown substrate:proton symporter, at 108,604 to 110,094
GFF6202
GFF6203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 110,246 to 111,229
GFF6203
Position (kb)
108
109
110
111 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 107.724 kb on + strand at 107.866 kb on - strand, within GFF6201 at 107.892 kb on + strand, within GFF6201 at 107.892 kb on + strand, within GFF6201 at 107.892 kb on + strand, within GFF6201 at 107.892 kb on + strand, within GFF6201 at 107.893 kb on - strand, within GFF6201 at 107.893 kb on - strand, within GFF6201 at 107.959 kb on + strand, within GFF6201 at 107.960 kb on - strand, within GFF6201 at 108.079 kb on - strand, within GFF6201 at 108.252 kb on + strand, within GFF6201 at 108.252 kb on + strand, within GFF6201 at 108.252 kb on + strand, within GFF6201 at 108.252 kb on + strand, within GFF6201 at 108.253 kb on - strand, within GFF6201 at 108.253 kb on - strand, within GFF6201 at 108.253 kb on - strand, within GFF6201 at 109.056 kb on - strand, within GFF6202 at 109.305 kb on - strand, within GFF6202 at 109.305 kb on - strand, within GFF6202 at 109.458 kb on - strand, within GFF6202 at 109.458 kb on - strand, within GFF6202 at 109.458 kb on - strand, within GFF6202 at 109.553 kb on + strand, within GFF6202 at 109.554 kb on - strand, within GFF6202 at 109.714 kb on + strand, within GFF6202 at 109.994 kb on + strand at 109.994 kb on + strand at 109.994 kb on + strand at 109.994 kb on + strand at 109.994 kb on + strand at 109.994 kb on + strand at 109.995 kb on - strand at 109.995 kb on - strand at 109.995 kb on - strand at 109.995 kb on - strand at 110.260 kb on - strand at 110.418 kb on + strand, within GFF6203 at 110.418 kb on + strand, within GFF6203 at 110.419 kb on - strand, within GFF6203 at 110.419 kb on - strand, within GFF6203 at 110.568 kb on + strand, within GFF6203 at 110.568 kb on + strand, within GFF6203 at 110.568 kb on + strand, within GFF6203 at 110.569 kb on - strand, within GFF6203 at 110.686 kb on - strand, within GFF6203 at 110.818 kb on - strand, within GFF6203
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 107,724 + -0.3 107,866 - GFF6201 0.12 -0.7 107,892 + GFF6201 0.15 -0.7 107,892 + GFF6201 0.15 -0.4 107,892 + GFF6201 0.15 +0.6 107,892 + GFF6201 0.15 +1.8 107,893 - GFF6201 0.15 +0.6 107,893 - GFF6201 0.15 -1.3 107,959 + GFF6201 0.23 -0.6 107,960 - GFF6201 0.23 -0.2 108,079 - GFF6201 0.38 -0.6 108,252 + GFF6201 0.59 +1.1 108,252 + GFF6201 0.59 -1.4 108,252 + GFF6201 0.59 +0.1 108,252 + GFF6201 0.59 -1.7 108,253 - GFF6201 0.59 +0.5 108,253 - GFF6201 0.59 -0.2 108,253 - GFF6201 0.59 +0.4 109,056 - GFF6202 0.30 +1.1 109,305 - GFF6202 0.47 -0.1 109,305 - GFF6202 0.47 -0.0 109,458 - GFF6202 0.57 +0.1 109,458 - GFF6202 0.57 -0.1 109,458 - GFF6202 0.57 +0.5 109,553 + GFF6202 0.64 -0.3 109,554 - GFF6202 0.64 -0.7 109,714 + GFF6202 0.74 -0.2 109,994 + +0.2 109,994 + +0.5 109,994 + -0.2 109,994 + -2.6 109,994 + +1.1 109,994 + -0.1 109,995 - +0.0 109,995 - -1.7 109,995 - -1.7 109,995 - -0.6 110,260 - -1.4 110,418 + GFF6203 0.17 -1.1 110,418 + GFF6203 0.17 -0.6 110,419 - GFF6203 0.18 -0.5 110,419 - GFF6203 0.18 -0.7 110,568 + GFF6203 0.33 -0.8 110,568 + GFF6203 0.33 -0.3 110,568 + GFF6203 0.33 +0.3 110,569 - GFF6203 0.33 -0.6 110,686 - GFF6203 0.45 -0.1 110,818 - GFF6203 0.58 +0.6
Or see this region's nucleotide sequence