Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6087 and GFF6088 are separated by 68 nucleotides GFF6088 and GFF6089 are separated by 149 nucleotides GFF6089 and GFF6090 are separated by 3 nucleotides GFF6090 and GFF6091 overlap by 4 nucleotides
GFF6087 - Transcriptional regulator, AcrR family, at 148,642 to 149,316
GFF6087
GFF6088 - Phenylacetic acid degradation protein PaaY, at 149,385 to 149,987
GFF6088
GFF6089 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18), at 150,137 to 150,940
GFF6089
GFF6090 - Acyl-coenzyme A thioesterase PaaD (Pse.pu.) (E. coli PaaI), at 150,944 to 151,402
GFF6090
GFF6091 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 151,399 to 152,709
GFF6091
Position (kb)
150
151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 149.162 kb on + strand, within GFF6087 at 149.162 kb on + strand, within GFF6087 at 149.162 kb on + strand, within GFF6087 at 149.162 kb on + strand, within GFF6087 at 149.162 kb on + strand, within GFF6087 at 149.163 kb on - strand, within GFF6087 at 149.163 kb on - strand, within GFF6087 at 149.163 kb on - strand, within GFF6087 at 149.163 kb on - strand, within GFF6087 at 149.163 kb on - strand, within GFF6087 at 149.163 kb on - strand, within GFF6087 at 149.165 kb on + strand, within GFF6087 at 149.165 kb on + strand, within GFF6087 at 149.166 kb on - strand, within GFF6087 at 149.166 kb on - strand, within GFF6087 at 149.166 kb on - strand, within GFF6087 at 149.166 kb on - strand, within GFF6087 at 149.171 kb on + strand, within GFF6087 at 149.171 kb on + strand, within GFF6087 at 149.171 kb on + strand, within GFF6087 at 149.172 kb on - strand, within GFF6087 at 149.172 kb on - strand, within GFF6087 at 149.291 kb on + strand at 149.292 kb on - strand at 149.292 kb on - strand at 149.337 kb on + strand at 149.337 kb on + strand at 149.337 kb on + strand at 149.337 kb on + strand at 149.337 kb on + strand at 149.337 kb on + strand at 149.337 kb on + strand at 149.338 kb on - strand at 149.338 kb on - strand at 149.338 kb on - strand at 149.338 kb on - strand at 149.338 kb on - strand at 149.512 kb on + strand, within GFF6088 at 149.512 kb on + strand, within GFF6088 at 149.513 kb on - strand, within GFF6088 at 149.513 kb on - strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.877 kb on + strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.878 kb on - strand, within GFF6088 at 149.881 kb on + strand, within GFF6088 at 149.881 kb on + strand, within GFF6088 at 149.881 kb on + strand, within GFF6088 at 149.881 kb on + strand, within GFF6088 at 149.881 kb on + strand, within GFF6088 at 149.881 kb on + strand, within GFF6088 at 149.882 kb on - strand, within GFF6088 at 149.935 kb on + strand at 149.935 kb on + strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.977 kb on + strand at 149.977 kb on + strand at 149.977 kb on + strand at 149.978 kb on - strand at 149.978 kb on - strand at 149.978 kb on - strand at 149.978 kb on - strand at 150.043 kb on + strand at 150.043 kb on + strand at 150.043 kb on + strand at 150.044 kb on - strand at 150.044 kb on - strand at 150.044 kb on - strand at 150.044 kb on - strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.047 kb on - strand at 150.047 kb on - strand at 150.047 kb on - strand at 150.153 kb on + strand at 150.153 kb on + strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.178 kb on - strand at 150.178 kb on - strand at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.411 kb on - strand, within GFF6089 at 150.411 kb on - strand, within GFF6089 at 150.411 kb on - strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.983 kb on + strand at 150.983 kb on + strand at 150.983 kb on + strand at 150.984 kb on - strand at 151.176 kb on - strand, within GFF6090 at 151.176 kb on - strand, within GFF6090 at 151.176 kb on - strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.352 kb on + strand, within GFF6090 at 151.528 kb on + strand at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.634 kb on - strand, within GFF6091 at 151.679 kb on + strand, within GFF6091 at 151.680 kb on - strand, within GFF6091 at 151.723 kb on + strand, within GFF6091 at 151.817 kb on - strand, within GFF6091 at 151.817 kb on - strand, within GFF6091 at 151.822 kb on + strand, within GFF6091 at 151.856 kb on - strand, within GFF6091
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 149,162 + GFF6087 0.77 -2.1 149,162 + GFF6087 0.77 -2.5 149,162 + GFF6087 0.77 +0.1 149,162 + GFF6087 0.77 +2.0 149,162 + GFF6087 0.77 +1.4 149,163 - GFF6087 0.77 -1.3 149,163 - GFF6087 0.77 -0.2 149,163 - GFF6087 0.77 -1.4 149,163 - GFF6087 0.77 -0.8 149,163 - GFF6087 0.77 -0.6 149,163 - GFF6087 0.77 +0.5 149,165 + GFF6087 0.77 -1.6 149,165 + GFF6087 0.77 -0.9 149,166 - GFF6087 0.78 -0.7 149,166 - GFF6087 0.78 +0.0 149,166 - GFF6087 0.78 +0.1 149,166 - GFF6087 0.78 -0.5 149,171 + GFF6087 0.78 -0.6 149,171 + GFF6087 0.78 -0.6 149,171 + GFF6087 0.78 -0.6 149,172 - GFF6087 0.79 -1.3 149,172 - GFF6087 0.79 -3.3 149,291 + -1.9 149,292 - +1.7 149,292 - +0.1 149,337 + -0.3 149,337 + -0.5 149,337 + -1.4 149,337 + -1.3 149,337 + +0.5 149,337 + +0.7 149,337 + -0.5 149,338 - +1.2 149,338 - -1.7 149,338 - -2.3 149,338 - +1.3 149,338 - -0.7 149,512 + GFF6088 0.21 +0.3 149,512 + GFF6088 0.21 -0.8 149,513 - GFF6088 0.21 -1.3 149,513 - GFF6088 0.21 +0.5 149,877 + GFF6088 0.82 -0.5 149,877 + GFF6088 0.82 +1.0 149,877 + GFF6088 0.82 +0.2 149,877 + GFF6088 0.82 -0.3 149,877 + GFF6088 0.82 -0.8 149,877 + GFF6088 0.82 -0.7 149,877 + GFF6088 0.82 +0.4 149,877 + GFF6088 0.82 -0.7 149,878 - GFF6088 0.82 -0.1 149,878 - GFF6088 0.82 +0.7 149,878 - GFF6088 0.82 -0.6 149,878 - GFF6088 0.82 +0.1 149,878 - GFF6088 0.82 +0.1 149,878 - GFF6088 0.82 -0.0 149,878 - GFF6088 0.82 +0.2 149,878 - GFF6088 0.82 -0.4 149,878 - GFF6088 0.82 +0.1 149,878 - GFF6088 0.82 +0.4 149,878 - GFF6088 0.82 +0.4 149,878 - GFF6088 0.82 -0.2 149,878 - GFF6088 0.82 -0.1 149,878 - GFF6088 0.82 +1.3 149,878 - GFF6088 0.82 -0.7 149,881 + GFF6088 0.82 -0.2 149,881 + GFF6088 0.82 -0.5 149,881 + GFF6088 0.82 -1.0 149,881 + GFF6088 0.82 -0.5 149,881 + GFF6088 0.82 -0.6 149,881 + GFF6088 0.82 -1.0 149,882 - GFF6088 0.82 +0.2 149,935 + +0.1 149,935 + -0.1 149,936 - -0.6 149,936 - +0.5 149,936 - +3.0 149,977 + -0.3 149,977 + +0.1 149,977 + +1.1 149,978 - -1.9 149,978 - -0.4 149,978 - -1.2 149,978 - -0.2 150,043 + -0.4 150,043 + -1.2 150,043 + -0.0 150,044 - -0.5 150,044 - +0.8 150,044 - +0.1 150,044 - -1.3 150,046 + +0.1 150,046 + -0.1 150,046 + -0.5 150,046 + -0.0 150,046 + +0.4 150,046 + -1.1 150,046 + -0.6 150,047 - -0.0 150,047 - +0.3 150,047 - -0.9 150,153 + -0.1 150,153 + +1.0 150,154 - +0.5 150,154 - -0.6 150,154 - -0.3 150,154 - -0.5 150,154 - +0.7 150,178 - +0.3 150,178 - -0.8 150,410 + GFF6089 0.34 -0.1 150,410 + GFF6089 0.34 -0.8 150,410 + GFF6089 0.34 +0.1 150,410 + GFF6089 0.34 -1.2 150,410 + GFF6089 0.34 +0.5 150,411 - GFF6089 0.34 -0.9 150,411 - GFF6089 0.34 +0.2 150,411 - GFF6089 0.34 -0.5 150,413 + GFF6089 0.34 -0.2 150,413 + GFF6089 0.34 +1.7 150,413 + GFF6089 0.34 +0.7 150,413 + GFF6089 0.34 +1.4 150,413 + GFF6089 0.34 -0.7 150,413 + GFF6089 0.34 -2.5 150,413 + GFF6089 0.34 -0.0 150,414 - GFF6089 0.34 -0.6 150,414 - GFF6089 0.34 -0.2 150,414 - GFF6089 0.34 -0.7 150,414 - GFF6089 0.34 -0.4 150,414 - GFF6089 0.34 -1.1 150,414 - GFF6089 0.34 -0.9 150,983 + -0.3 150,983 + -0.1 150,983 + -0.8 150,984 - -0.3 151,176 - GFF6090 0.51 -1.5 151,176 - GFF6090 0.51 +0.4 151,176 - GFF6090 0.51 +0.6 151,292 + GFF6090 0.76 +1.1 151,292 + GFF6090 0.76 -0.1 151,292 + GFF6090 0.76 +0.7 151,292 + GFF6090 0.76 -1.3 151,292 + GFF6090 0.76 -0.2 151,293 - GFF6090 0.76 +0.1 151,293 - GFF6090 0.76 -0.3 151,293 - GFF6090 0.76 +0.5 151,293 - GFF6090 0.76 -0.7 151,293 - GFF6090 0.76 -2.4 151,293 - GFF6090 0.76 +0.3 151,293 - GFF6090 0.76 -1.3 151,352 + GFF6090 0.89 -0.4 151,528 + -0.6 151,633 + GFF6091 0.18 +1.0 151,633 + GFF6091 0.18 -0.1 151,633 + GFF6091 0.18 +0.2 151,633 + GFF6091 0.18 -0.5 151,633 + GFF6091 0.18 +0.2 151,634 - GFF6091 0.18 -0.0 151,679 + GFF6091 0.21 -0.6 151,680 - GFF6091 0.21 +0.1 151,723 + GFF6091 0.25 +1.2 151,817 - GFF6091 0.32 +0.2 151,817 - GFF6091 0.32 +0.7 151,822 + GFF6091 0.32 -0.7 151,856 - GFF6091 0.35 +0.4
Or see this region's nucleotide sequence